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A Recent Overview of Microbes and Microbiome Preservation

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Abstract

Microbes are mediators in almost all ecosystem processes and act as a pivotal game changer in various ecological activities, globally. Therefore, understanding of microbial community structure and related functions in different environmental and micro-environmental niches is not only critical, but also a matter of greatest importance. Due to our inability to cultivate and preserve all sorts of microorganisms, we are losing some ecologically and industrially relevant components of microbial community, due to extinction caused by environmental and climatic variations with time. Intact sample and microbiome preservation are crucial for future cultivation as well as to study the effects of ecological and climatic variations on community functionality and shift with time, using OMICS. Although, methods for pure culture preservation are almost optimized, the techniques of microbiome preservation still remain as an unsolved challenge for microbiologists due to technical and physiological constraints. Present article discusses, recent approaches of microbial preservation with special reference to intact sample, mixed culture and microbiome preservation. It also incorporates recent practices used to achieve the highest viability and metabolic activities in long-term preserved microbiome.

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Acknowledgements

This work was supported from the Grant No. BT/PR13969/BCE/8/1142/2015, Department of Biotechnology (DBT), Govt. of India. Dhananjay Desai acknowledges principal investigator (AICRP-ADMAS) and Director, ICAR-CCARI Ela, Old Goa, Goa 403402 for permitting him to work on the manuscript. We acknowledge the critical editing of manuscript by Manali Vaijanapurkar.

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  1. National Centre for Microbial Resource (NCMR), National Centre for Cell Science (NCCS), Pune, Maharashtra, 411007, India

    Om Prakash & Yogesh Nimonkar

  2. Animal Science Section, ICAR-Central Coastal Agricultural Research Institute, Ela, Old Goa, Goa, 403402, India

    Dhananjay Desai

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Prakash, O., Nimonkar, Y. & Desai, D. A Recent Overview of Microbes and Microbiome Preservation. Indian J Microbiol 60, 297–309 (2020). https://doi.org/10.1007/s12088-020-00880-9

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