Skip to main content
Log in

The effect of acidic-treated acorn pollen on lipid and antioxidant metabolism with ovariectomized rats

  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

The hormonal imbalance that occurs during menopause increases the risk of aging and chronic diseases. Therefore, this study was intended to improve the health of menopause women by using pollen, which is a natural food, not drugs and hormones. Experiment I provided the supernatant of pollen vinegar extract with oral administration using ovariectomized rats. Experiment II used ovariectomized rats to provide pollen vinegar sediment mixed with the AIN-93 M diet and then looked into the effects on antioxidant and lipid metabolism. As a result, the intake of pollen controlled the lipid concentration and reduced the risk of arteriosclerosis. It inhibited the release of inflammable substances from hepatic and adipocyte tissues, and confirmed the anti-aging effect. These results indicated that pollen is effective in preventing obesity, metabolic diseases and aging after menopause.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Aebi H. Catalase. pp. 673-677. In: Methods of Enzymatic Analysis. 2th ed. Academic Press, NY, USA (1974)

    Book  Google Scholar 

  • Andrade P, Ferreres F, Gil MI, Tomas-Barberan FA. Determination of phenolic compounds in honeys with different floral origin by capillary zone electrophoresis. Food Chem. 60: 79-84 (1997)

    Article  CAS  Google Scholar 

  • Beheshti Z, Huak Chan Y, Sharif Nia H, Hajihosseini F, Nazari R, Shaabani M, Salehi Omran MT. Influence of apple cider vinegar on blood lipids. Life Sci. J. 9: 2431-2440 (2012)

    Google Scholar 

  • Block G, Sinha R, Gridley G. Collection of dietary-supplement data and implication for analysis. Am. J. Clin. Nutr. 59: 232S-239S (1994)

    Article  CAS  Google Scholar 

  • Bouazza A, Bitam A, Amiali M, Bounihi A, Yargui L, Koceir EA. Effect of fruit vinegars on liver damage and oxidative stress in high-fat-fed rats. Pharm. Biol. 8: 1-6 (2015)

    Google Scholar 

  • Campos MG, Webby RF, Markham KR, Mitchell KA, Cunha AP. Age-induced diminution of free radical scavenging capacity in bee pollens and the contribution of constituent flavonoids. J. Agric. Food Chem. 51: 742-745 (2003)

    Article  CAS  Google Scholar 

  • Choi Il, Chang HS, Lee E. Effects of Trichosanthis Radix ext. on anti-oxidation, biochemical and biological values of blood in broiler chicks. J. Agric. Life Sci. 47: 61-69 (2013)

  • Dipiro JT, Talbert RL, Yee GC, Matzke GR, Wells BG, Michael Posey L. Pharmacotherapy: A pathophysiologic approach. 10th ed. McGraw-Hill, NY, USA (2016)

    Google Scholar 

  • Georgiev DB, Metka M, Huber JC, Goudev AR, Manassiev N. Effects of an herbal medication containing bee products on menopausal symptoms and cardiovascular risk markers: Results of a pilot open-uncontrolled trial. Medscape J. Med. 6: 46 (2004)

    Google Scholar 

  • Gibson DM, Hubbard DD. Incorporation of malonyl CoA into fatty acids by liver in starvation and alloxan-diabetes. Biochem. Biophys. Res. Commun. 3: 531-535 (1960)

    Article  CAS  Google Scholar 

  • Grady D, Herrington D, Bittner V, Blumenthal R, Davidson M, Hlatky M, Hsia J, Hulley S, Herd A, Khan S, Newby LK, Waters D, Vittinghoff E, Wenger N. Cardiovascular disease outcomes during 6.8 years of hormone therapy: heart and estrogen/progestin replacement study follow-up (HERS II). Jama 288: 49-57 (2002)

  • Havel PJ. Control of energy homeostasis and insulin action by adipocyte hormones; leptin, acylation stimulating protein and adiponectin. Curr. Opin. Lipidol. 13: 51-59 (2002)

    Article  CAS  Google Scholar 

  • Henderson BE, Ross R, Bernstein L. Estrogens as a cause of human cancer: The richard and hinda rosenthal foundation award lecture. Cancer Res. 48: 246-253 (1988)

    CAS  PubMed  Google Scholar 

  • Holst D, Grimaldi PA. New factors in the regulation of adipose differentiation and metabolism. Curr. Opin. Lipidol. 13: 241-245 (2002)

    Article  CAS  Google Scholar 

  • Hong IP, Woo SO, Han SM, Yeo JH, Cho ML. Germination and antioxidant activity of korean oak pollen treated with medicinal mushroom. Kor. J. Mycol. 42: 165-169 (2014)

    Article  Google Scholar 

  • Hsu CL, Yen GC. Effects of flavonoids and phenolic acids on the inhibition of adipogenesis in 3T3-L1 adipocytes. J. Agric. Food Chem. 55: 8404-8410 (2007)

    Article  CAS  Google Scholar 

  • Kafadar IH, Güney A, Türk CY, Oner M, Silici S. Royal jelly and bee pollen decrease bone loss due to osteoporosis in an oophorectomized rat model. Eklem Hastalik Cerrahisi. 23: 100-105 (2012)

    PubMed  Google Scholar 

  • Kim BY, Kim MH. The effect of Ishige okamurae extracts on antioxidant activity and serum lipid content in ovariectomized rats. J. Life Sci. 23: 1501-1508 (2013)

    Article  Google Scholar 

  • Kroyer G, Hegedus N. Evaluation of bioactive properties of pollen extracts as functional dietary food supplement. Innov. Food Sci. Emerg. Technol. 2: 171-174 (2001)

    Article  CAS  Google Scholar 

  • Lee BC, Park HM, Sim HS, Kim GS, Gu JH, OH MJ. Biological activity and chemical analysis of cattail pollens. Korean J. Agric. Sci. 36: 185-197 (2009)

  • Lee JH, Cho HD, Jeong JH, Lee MK, Jeong YK, Shim KH, Seo KI. New vinegar produced by tomato suppresses adipocyte differentiation and fat accumulation in 3T3-L1 cells and obese rat model. Food Chem. 141: 3241-3249 (2013)

    Article  CAS  Google Scholar 

  • Lee MK, Choi SR, Lee J, Choi YH, Lee JH, Park KU, Kwon SH, Seo KI. Quality characteristics and anti-diabetic effect of yacon vinegar. J. Korean Soc. Food Sci. Nutr. 41: 79-86 (2012)

    Article  CAS  Google Scholar 

  • Lee YH, Lim W, Sung MK. Carnosic acid modulates increased hepatic lipogenesis and adipocytes differentiation in ovariectomized mice fed normal or high-fat diets. Nutrients 10: 1-14 (2018)

    CAS  Google Scholar 

  • Li F, Yuan Q, Rashid F. Isolation, purification and immunobiological activity of a new water-soluble bee pollen pplysaccharide from Crataegus pinnatifidia Bge. Carbohydr. Polym. 78: 80-88 (2009)

    Article  CAS  Google Scholar 

  • Liu XD, Zhang FB, Zhou B, Shan H, Chen PY. Effect of sonication on different quality parameters of pinus massoniana pollen. Ultrason. Sonochem. 22: 174-181 (2015)

    Article  CAS  Google Scholar 

  • Lowdermilk DL, Perry SE, Cashion MC, Alden KR. Maternity and women’s health care. 11th ed. Elsevier, Mosby, USA. pp. 132-135 (2014)

    Google Scholar 

  • Mackness MI, Arrol S, Durrington PN. Paraoxonase prevents accumulation of lipoperoxides in low-density lipoprotein. FEBS. Lett. 286: 152-154 (1991)

    Article  CAS  Google Scholar 

  • Nazıroglu M, Guler M, Ozgul C, Saydam G, Kuçukayaz M, Sozbir E. Apple cider vinegar modulates serum lipid profile, erythrocyte, kidney, and liver membrane oxidative stress in ovariectomized mice fed high cholesterol. J. Membr. Biol. 247: 667-673 (2014)

    Article  Google Scholar 

  • Newton KM, Reed SD, Grothaus L, Ehrlich K, Guiltinan J, Ludman E, Lacroix AZ. The herbal alternatives for menopause study (HALT): background and study design. Maturitas 52: 134-146 (2005)

    Article  Google Scholar 

  • Noorafshan A, Dabbaghmanesh MH, Tanideh N, Koohpeyma F, Rasooli R, Hajihoseini M, Bakhshayeshkaram M, Hosseinabadi OK. Stereological study of the effect of black olive hydroalcoholic extract on osteoporosis in vertebra and tibia in ovariectomized rats. Osteoporos. Int. 26: 2299-2307 (2015)

    Article  CAS  Google Scholar 

  • Osmers R, Friede M, Liske E, Schnitker J, Freudenstein J, Henneicke-von Z, Hans-Heinrich. Efficacy and safety of isopropanolic black cohosh extract for climactericsymptoms. Obstet. Gynecol. 105: 1074-1083 (2005)

  • Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70: 158-169 (1967)

    CAS  PubMed  Google Scholar 

  • Park YS, Kim MH. The effect of eisenia bicyclis extracts on antioxidant activity and serum lipid level in ovariectomized rats. J. Life Sci. 22: 1407-1414 (2012)

    Article  Google Scholar 

  • Rivera L, Moron R, Sanchez M, Zarzuelo A, Galisteo M. Quercetin ameliorates metabolic syndrome and improves the inflammatory status in obese zucker rats. Obesity. 16: 2081-2087 (2008)

    Article  CAS  Google Scholar 

  • Ross R. Atherosclerosis-An Inflammatory Disease. N. Engl. J. Med. 340: 115-126 (1999)

    Article  Google Scholar 

  • Roulston TH, Cane JH. Pollen nutritional content and digestibility for animals. Plant Syst. Evol. 222: 187-209 (2000)

    Article  CAS  Google Scholar 

  • Saric A, Balog T, Sobocanec S, Kusic B, Sverko V, Rusak G, Likic S, Bubalo D, Pinto B, Reali D, Marotti T. Antioxidant effects of flavonoid from Croatian Cystus incanus L. rich bee pollen. Food Chem. Toxicol. 47: 547-554 (2009)

  • Seydim AC, Guzel-Seydim ZB, Doguc DK, Savas MC, Budak HN. Effects of grape wine and apple cider vinegar on oxidative and antioxidative status in high cholesterol-fed rats. Funct. Foods Health Dis. 6: 569-577 (2016)

    Article  CAS  Google Scholar 

  • Stubbins RE, Holcomb VB, Hong J, Núñez NP. Estrogen modulates abdominal adiposity and protects female mice from obesity and impaired glucose tolerance. Eur. J. Nutr. 51: 861-870 (2012)

    Article  CAS  Google Scholar 

  • Tatsimo SJN, Tamokou JDD, Havyarimana L, Csupor D, Forgo P, Hohmann J, Kuiate JR, Tane P. Antimicrobial and antioxidant activity of kaempferol rhamnoside derivatives from Bryophyllum pinnatum. BMC Res. Notes. 5: 158 (2012)

    Article  CAS  Google Scholar 

  • Toth MJ, Tchernof A, Sites CK, Poehlman ET. Effect of menopausal status on body composition and abdominal fat distribution. Int. J. Obes. 24: 226-231 (2000)

    Article  CAS  Google Scholar 

  • Yamashita H, Fujisawa K, Ito E, Idei S, Kawaguchi N, Kimoto M, Hiemori M, Tsuji H. Improvement of obesity and glucose tolerance by acetate in type 2 diabetic otsuka long-evans tokushima fatty (OLETF) rats. Biosci. Biotechnol. Biochem. 71: 1236-1243 (2007)

    Article  CAS  Google Scholar 

  • Yoon SH, Kang JH, Kwon CS. Effects of Azalea (Rhododendron mucronulatum) pollen on the carbon tetrachloride induced hepatotoxicity in mice. J. Korean Soc. Food Sci. Nutr. 18: 363-370 (1989)

    Google Scholar 

  • Wakil SJ, Stoops JK, Joshi VC. Fatty acid synthesis and its regulation. Annu. Rev. Biochem. 52: 537-579 (1983)

    Article  CAS  Google Scholar 

  • Zuluaga C, Serrato JC, Quicazan, M. Bee-Pollen structure modification by physical and biotechnological processing: influence on the availability of nutrients and bioactive compounds. Chem. Eng. Trans. 43: 79-84 (2015)

    Google Scholar 

Download references

Acknowledgements

This research was supported by the Cooperative Research Program for Agriculture Science and Technology Development Project (No. PJ013140), Rural Development Administration, Republic of Korea.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mi Young Kang.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nam, S.J., Chung, S.I. & Kang, M.Y. The effect of acidic-treated acorn pollen on lipid and antioxidant metabolism with ovariectomized rats. Food Sci Biotechnol 29, 1081–1089 (2020). https://doi.org/10.1007/s10068-020-00751-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-020-00751-5

Keywords

Navigation