Abstract
Selenium is an essential micronutrient that plays an important role in immunity. However, the mechanism that Selenium modulates mastitis is not fully clear. In this experiment, we investigated whether selenium can inhibit the activation of the NLRP3 inflammasome in a mouse model of Staphylococcus aureus-induced mastitis. Eighty BALB/c female mice were fed with experimental Selenium deficiency basal diet for 2 weeks to achieve the purpose of selenium consumption until pregnancy. Pregnant mice were randomly divided into four groups (control group; selenium supplement group; Staphylococcus aureus infection group and Staphylococcus aureus infection after selenium supplement group). Twenty-four hours after challenging, all mice were euthanized and mammary tissue samples were aseptically collected. Through pathological staining, western blot analysis, real-time fluorescence quantitative polymerase chain reaction analysis, and enzyme-linked immunosorbent assay, the regulation effect of Selenium on NLRP3 inflammasome was detected. The result showed that compared with the control group, selenium significantly inhibited the expression of NLRP3, ASC, Caspase-1, Caspase-1 p20, and Pro-IL-1β (p < 0.01). Meanwhile the mRNA expression and release of IL-1β was suppressed in the treatment group compared with Staphylococcus aureus infection group (p < 0.01). Therefore, these results suggest that dietary selenium can attenuate Staphylococcus aureus mastitis by inhibition of the NLRP3 inflammasome.
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Funding
This work was supported by the National Natural Science Foundation of China (NO. 31802254), the Science and Technology Project of Shandong Province Higher Education Institutions (J18KB074), and the Key Research and Development Project of Hebei (19226625D).
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Chong-Liang Bi contributed to the overall study design and supervised all research. Shu-Jiu Zhang carried out the experiments and analyzed the data and was also responsible for the final editing of the manuscript. Yi-Zhao Shen contributed partly to the writing and finally revising the manuscript and data analysis. Mirielle Pauline contributed to language editing and revision. Hui Li prepared the figures. He Tang drafted and revised the first version of the manuscript. All the authors reviewed and finally approved the manuscript.
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All experimental procedures were conducted with the approval of the Institutional Animal Care and Use Committee of Linyi University (Approval ID: [2006], 24).
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Bi, CL., Zhang, SJ., Shen, YZ. et al. Selenium Plays an Anti-Inflammatory Role by Regulation NLRP3 Inflammasome in Staphylococcus aureus-Infected Mouse Mammary Gland. Biol Trace Elem Res 199, 604–610 (2021). https://doi.org/10.1007/s12011-020-02166-z
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DOI: https://doi.org/10.1007/s12011-020-02166-z