Skip to main content

Advertisement

SpringerLink
Log in

Biofilm Eradication Activity of Herb and Spice Extracts Alone and in Combination Against Oral and Food-Borne Pathogenic Bacteria

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

The purpose of this study was to select herbs and spices with potent biofilm eradication activities. Further, the combined effects of herb and spice extracts against pathogenic biofilms were evaluated. The biofilm eradication activities of ethanol extracts of 104 herbs and spices were measured by combining a colorimetric microbial viability assay with a biofilm formation technique. Ethanol extract of clove had potent biofilm eradication activities against Escherichia coli, Porphyromonas gingivalis, and Streptococcus mutans. Ethanol extracts of eucalyptus and rosemary had potent biofilm eradication activities against P. gingivalis, Staphylococcus aureus and S. mutans. The combination of extracts of clove with eucalyptus or rosemary showed synergistic or additive effects, or both, on biofilm eradication activities. The main biofilm inhibitors in the ethanol extracts of clove, eucalyptus and rosemary were eugenol, macrocarpals and carnosic acid, respectively. The combinations of extracts of clove with eucalyptus or rosemary had potent biofilm eradication activities against oral and food-borne pathogenic bacteria. The findings of the present study reveal that specific combinations of herb and spice extracts may prevent and control biofilm-related oral diseases, food spoilage, and food poisoning.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Simões M, Simões LC, Vieira MJ (2010) A review of current and emergent biofilm control strategies. LWT - Food Sci Technol 43:573–583. https://doi.org/10.1016/j.lwt.2009.12.008

    Article  CAS  Google Scholar 

  2. How KY, Song KP, Chan KG (2016) Porphyromonasgingivalis: An overview of periodontopathic pathogen below the gum line. Front Microbiol. https://doi.org/10.3389/fmicb.2016.00053

    Article  PubMed  PubMed Central  Google Scholar 

  3. Marsh PD, Head DA, Devine DA (2015) Dental plaque as a biofilm and a microbial community - Implications for treatment. J Oral Biosci 57:185–191. https://doi.org/10.1016/j.job.2015.08.002

    Article  Google Scholar 

  4. Strużycka I (2014) The oral microbiome in dental caries. Pol J Microbiol 63:127–135

    Article  Google Scholar 

  5. Jahid IK, Ha S (2012) A review of microbial biofilms of produce: Future challenge to food safety. Food Sci Biotechnol 21:299–316. https://doi.org/10.1007/s10068-012-0041-1

    Article  CAS  Google Scholar 

  6. Gutierrez D, Delgado S, Vazquez-Sanchez D, Martinez B, Cabo ML, Rodriguez A, Herrera JJ, Garcia P (2012) Incidence of Staphylococcus aureus and analysis of associated bacterial communities on food industry surfaces. Appl Environ Microbiol 78:8547–8554. https://doi.org/10.1128/AEM.02045-12

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. Leja KB, Czaczyk K (2016) The industrial potential of herbs and spices – A mini review. Acta Sci Pol Technol Aliment 15:353–365. https://doi.org/10.17306/J.AFS.2016.4.34

    Article  PubMed  Google Scholar 

  8. Kim HS, Park HD (2013) Ginger extract inhibits biofilm formation by Pseudomonas aeruginosa PA14. PLoS ONE. https://doi.org/10.1371/journal.pone.0076106

    Article  PubMed  PubMed Central  Google Scholar 

  9. Sandasi M, Leonard CM, Viljoen AM (2010) The in vitro antibiofilm activity of selected culinary herbs and medicinal plants against Listeria monocytogenes. Lett Appl Microbiol 50:30–35. https://doi.org/10.1111/j.1472-765X.2009.02747.x

    Article  PubMed  CAS  Google Scholar 

  10. Stojanović-Radić Z, Pejčić M, Stojanović N, Sharifi-Rad J, Stanković N (2016) Potential of Ocimum basilicum L. and Salvia officinalis L. essential oils against biofilms of P. aeruginosa clinical isolates. Cell Mol Biol 62:27–33

    PubMed  Google Scholar 

  11. Szczepanski S, Lipski A (2014) Essential oils show specific inhibiting effects on bacterial biofilm formation. Food Cont 36:224–229. https://doi.org/10.1016/j.foodcont.2013.08.023

    Article  CAS  Google Scholar 

  12. Tsukatani T, Kawaguchi T, Suenaga H, Shiga M, Ikegami T (2016) Rapid and simple determination of minimum biofilm eradication concentration by a colorimetric microbial viability assay based on reduction of a water-soluble tetrazolium salt and combined effect of antibiotics against microbial biofilm. J Microbiol Biotechnol Food Sci 6:677–680. https://doi.org/10.15414/jmbfs.2016.6.1.677-680

    Article  CAS  Google Scholar 

  13. Tsukatani T, Suenaga H, Higuchi T, Akao T, Ishiyama M, Ezoe T, Matsumoto K (2009) Colorimetric microbial viability assay based on reduction of water-soluble tetrazolium salts for antimicrobial susceptibility testing and screening of antimicrobial substances. Anal Biochem 393:117–125. https://doi.org/10.1016/j.ab.2009.06.026

    Article  PubMed  CAS  Google Scholar 

  14. Pundir RK, Jain P, Sharma C (2010) Antimicrobial activity of ethanolic extracts of Syzygium aromaticum and Allium sativum against food associated bacteria and fungi. Ethnobot Leaflets 14:344–360

    Google Scholar 

  15. Sueke H, KayeNeal SB, Hall A, Tuft S, Parry CM (2010) An in vitro investigation of synergy or antagonism between antimicrobial combinations against isolates from bacterial keratitis. Invest Ophthalmol Vis Sci 51:4151–4155. https://doi.org/10.1167/iovs.09-4839

    Article  PubMed  Google Scholar 

  16. Agrawal N, Gupta R, Gupta I, Mehrotra V, Roopa DA (2014) Herbcraft: boon to the periodontal therapy. Int J Dent Health Sci 1:47–62

    Google Scholar 

  17. Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods - a review. Int J Food Microbiol 94:223–253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022

    Article  PubMed  CAS  Google Scholar 

  18. Cortés-Rojas DF, De Souza CRF, Oliveira WP (2014) Clove (Syzygium aromaticum): a precious spice. Asian Pac J Trop Biomed 4:90–96. https://doi.org/10.1016/S2221-1691(14)60215-X

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  19. Bayoub K, Baibai T, Mountassif D, Retmane A, Soukri A (2010) Antibacterial activities of the crude ethanol extracts of medicinal plants against Listeria monocytogenes and some other pathogenic strains. Afr J Biotechnol 9:4251–4258

    Google Scholar 

  20. Gupta C, Garg A, Uniyal R, Gupta S (2008) Comparison of antimicrobial activities of clove oil & its extract on some food borne microbes. Internet J Microbiol 7:7

    Google Scholar 

  21. Mirpour M, Siahmazgi ZG, Kiasaraie MS (2015) Antibacterial activity of clove, gall nut methanolic and ethanolic extracts on Streptococcus mutans PTCC 1683 and Streptococcus salivarius PTCC 1448. J Oral Biol Craniofac Res 5:7–10. https://doi.org/10.1016/j.jobcr.2015.02.002

    Article  PubMed  PubMed Central  Google Scholar 

  22. Nagata H, Inagaki Y, Yamamoto Y, Maeda K, Kataoka K, Osawa K, Shizukuishi S (2006) Inhibitory effects of macrocarpals on the biological activity of Porphyromonas gingivalis and other periodontopathic bacteria. Mol Oral Microbiol 21:159–163. https://doi.org/10.1111/j.1399-302X.2006.00269.x

    Article  CAS  Google Scholar 

  23. Sebei K, Sakouhi F, Herchi W, Khouja ML, Boukhchina S (2015) Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves. Biol Res. https://doi.org/10.1186/0717-6287-48-7

    Article  PubMed  PubMed Central  Google Scholar 

  24. Takahashi T, Kokubo R, Sakaino M (2004) Antimicrobial activities of eucalyptus leaf extracts and flavonoids from Eucalyptus maculate. Lett Appl Microbiol 39:60–64. https://doi.org/10.1111/j.1472-765X.2004.01538.x

    Article  PubMed  CAS  Google Scholar 

  25. Wong JH, Lau KM, Wu YO, Cheng L, Wong CW, Yew DTW, Leung PC, Fung KP, Hui M, Ng TB, Lau CBS (2015) Antifungal mode of action of macrocarpal C extracted from Eucalyptus globulus Labill (Lan An) towards the dermatophyte Trichophyton mentagrophytes. Chin Med. https://doi.org/10.1186/s13020-015-0068-3

    Article  PubMed  PubMed Central  Google Scholar 

  26. Yamakoshi Y, Murata M, Shimizu A, Homma S (1992) Isolation and characterization of macrocarpals B-G antibacterial compounds from Eucalyptus macrocarpa. Biosci Biotechnol Biochem 56:1570–1576. https://doi.org/10.1271/bbb.56.1570

    Article  PubMed  CAS  Google Scholar 

  27. Bernardes WA, Lucarini R, Tozatti MG, Souza MGM, Silva MLA, Filho AAS, Martins GHG, Crotti AEM, Pauletti PM, Groppo M, Cunha WR (2010) Antimicrobial activity of Rosmarinus officinalis against oral pathogens: relevance of carnosic acid and carnosol. Chem Biodivers 7:1835–1840

    Article  CAS  Google Scholar 

  28. Birtic S, Dussort P, Pierre FX, Bily AC, Roller M (2015) Carnosic acid. Phytochemistry 115:9–19. https://doi.org/10.1016/j.phytochem.2014.12.026

    Article  PubMed  CAS  Google Scholar 

  29. Jordán MJ, Lax V, Rota MC, Lorán S, Sotomayor JA (2012) Relevance of carnosic acid, carnosol, and rosmarinic acid concentrations in the in vitro antioxidant and antimicrobial activities of Rosmarinus officinalis (L.) methanolic extracts. J Agric Food Chem 60:9603–9608. https://doi.org/10.1021/jf302881t

    Article  PubMed  CAS  Google Scholar 

  30. Kavanaugh KL, Ribbeck K (2012) Selected antimicrobial essential oils eradicate Pseudomonas spp. and Staphylococcus aureus biofilms. Appl Environ Microbiol 78:4057–4061. https://doi.org/10.1128/AEM.07499-11

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  31. Genena AK, Hense H, Smânia A Jr, De Souza SM (2008) Rosemary (Rosmarinus officinalis) - a study of the composition, antioxidant and antimicrobial activities of extracts obtained with supercritical carbon dioxide. Ciênc Tecnol Aliment Campinas 28:463–469. https://doi.org/10.1590/S0101-20612008000200030

    Article  CAS  Google Scholar 

  32. Behbahani BA, Yazdi FT, Mortazavi A, Zendeboodi F, Gholian MM, Vasiee A (2013) Effect of aqueous and ethanolic extract of Eucalyptus camaldulensis L. on food infection and intoxication microorganisms “in vitro”. J Paramed Sci 4:88–99

    Google Scholar 

  33. Devi KP, Nisha SA, Sakthivel R, Pandian SK (2010) Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. J Ethnopharmacol 130:107–115. https://doi.org/10.1016/j.jep.2010.04.025

    Article  PubMed  CAS  Google Scholar 

  34. Oyedemi SO, Okoh AI, Mabinya LV, Pirochenva G, Afolayan AJ (2009) The proposed mechanism of bactericidal action of eugenol, α-terpineol and γ-terpinene against Listeria monocytogenes, Streptococcus pyogenes, Proteus vulgaris and Escherichia coli. Afr J Biotechnol 8:1280–1286

    CAS  Google Scholar 

  35. Ojeda-Sana AM, Repetto V, Moreno S (2013) Carnosic acid is an efflux pumps modulator by dissipation of the membrane potential in Enterococcus faecalis and Staphylococcus aureus. World J Microbiol Biotechnol 29:137–144. https://doi.org/10.1007/s11274-012-1166-3

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This research was financially supported by the Yamazaki Spice Promotion Foundation (224).

Author information

Authors and Affiliations

  1. Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawamachi, Kurume, 839-0861, Japan

    Tadayuki Tsukatani, Fumihiko Sakata & Rieko Kuroda

  2. Fukuoka Industrial Technology Center, Chikushino, Japan

    Tetsuyuki Akao

Authors
  1. Tadayuki Tsukatani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fumihiko Sakata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rieko Kuroda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tetsuyuki Akao
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

TT designed the experiments. TT and FS carried out experimental work. RK and TA participated in the sample collection. TT wrote the manuscript. RK and TA advised on the manuscript content. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Tadayuki Tsukatani.

Ethics declarations

Conflicts of interest

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tsukatani, T., Sakata, F., Kuroda, R. et al. Biofilm Eradication Activity of Herb and Spice Extracts Alone and in Combination Against Oral and Food-Borne Pathogenic Bacteria. Curr Microbiol 77, 2486–2495 (2020). https://doi.org/10.1007/s00284-020-02017-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-020-02017-z

Search

Navigation