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Hydrogel Production Platform with Dynamic Movement Using Photo-Crosslinkable/Temperature Reversible Chitosan Polymer and Stereolithography 4D Printing Technology

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Three-dimensional (3D) printing using hydrogel has made great strides when it comes to mimicking 3D artificial tissue in the medical field. However, most structures do not mimic the dynamic movement of the tissues. Without imitating dynamic movements, there are limitations on the extent to which the proper implementation of the tissue's own functions can be achieved.

Method:

In this study, we intend to present an approach to solving this problem using hydroxybutyl methacrylated chitosan (HBC-MA), a photo-crosslinkable/temperature reversible chitosan polymer. In addition, stereolithography-3D (SLA-3D) printing technology was used, which is more likely to mimic the complex microstructure. As a control, a 3D structure made with pristine poly(ethylene glycol) dimethacrylate (PEG-DMA) was created, and a 4D structure was prepared by adding HBC-MA to poly(ethylene glycol) dimethacrylate (PEG-DMAP) resin.

Results:

HBC-MA caused the expansion of water into the polymer matrix at low temperature, and the 4D structure resulted in expansion of the polymer volume, generating dynamic movement due to the expansion of water. Conversely, as the temperature rose, deswelling occurred, followed by a decrease in the volume, showing a shape memory property of returning to the existing structure. Morphological, swelling, and mechanical analysis further confirmed the principle of dynamic movement. In addition, parameters were provided through calculation of the bending ratio angle (θ).

Conclusion:

Through this, it is suggested that HBC-MA can be applied as a core polymer for SLA-4D printing, and has high potential for realizing the dynamic movement of tissue.

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Acknowledgements

This paper was funded by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIT) (NRF-2015R1A5A1009701, NRF-2018R1D1A1B05047274, and NRF-2016M3A9B6946859), and was supported by Konkuk University Researcher Fund in 2018.

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Authors and Affiliations

  1. Department of Stem Cell and Regenerative Biotechnology, KU Convergence Science and Technology Institute, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul, 05029, Republic of Korea

    Jeong Wook Seo & Hojae Bae

  2. Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, 02139, USA

    Su Ryon Shin

  3. Department Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul, 05029, Republic of Korea

    Yeon Joo Park

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  1. Jeong Wook Seo
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Correspondence to Hojae Bae.

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Seo, J.W., Shin, S.R., Park, Y.J. et al. Hydrogel Production Platform with Dynamic Movement Using Photo-Crosslinkable/Temperature Reversible Chitosan Polymer and Stereolithography 4D Printing Technology. Tissue Eng Regen Med 17, 423–431 (2020). https://doi.org/10.1007/s13770-020-00264-6

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  • DOI: https://doi.org/10.1007/s13770-020-00264-6

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