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Gibberellin-Induced Alterations to the Expression of Cell Wall-Related Genes in the Xylem of Carrot Root

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Abstract

Gibberellins (GAs) are a group of plant hormones that play important roles in various processes. Previous studies demonstrated that GA can increase the cellulose content and lignification of plant tissues. However, the effects of altered GA levels on carrot root development have not been fully characterized. In this study, carrot roots were treated with exogenous GA3, after which thickened cell walls were detected in the xylem area. Transcriptome sequencing data revealed the changes induced by GA3. A total of 5416 differentially expressed genes (DEGs) were identified, of which 3011 and 2405 were up- and down-regulated, respectively. The DEGs were enriched in metabolic pathways, with 236 DEGs related to the cell wall. Digital gene expression data and the verification by qRT-PCR indicated that exogenous GA can alter the expression of genes involved in cell wall synthesis, suggesting a potential role for GA in cell wall organization and modification. The results of this study provide novel insights into the GA functions influencing carrot root development.

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Acknowledgements

This study was funded by the Natural Science Foundation of Jiangsu Province (BK20170460), Scientific Research Foundation for Doctor from Huaiyin Institute of Technology (Z301B16531), New Century Excellent Talent of the Ministry of Education (NCET-11-0670), and Jiangsu Natural Science Foundation for Distinguished Young Scholars (BK20130027).

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Authors and Affiliations

  1. School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China

    Guang-Long Wang, Ya-Hong An, Ji-Zhong Wang & Min Sun

  2. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Ya-Hui Wang, Jie-Xia Liu & Ai-Sheng Xiong

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  1. Guang-Long Wang
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Contributions

GLW and ASX initiated and designed the research. GLW, YHW, and JXL performed the experiments. YHA, MS, and JZW analyzed the data. GLW and ASX revised the paper. All authors read and approved the final manuscript.

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Correspondence to Guang-Long Wang or Ai-Sheng Xiong.

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Wang, GL., An, YH., Wang, YH. et al. Gibberellin-Induced Alterations to the Expression of Cell Wall-Related Genes in the Xylem of Carrot Root. J Plant Growth Regul 40, 787–797 (2021). https://doi.org/10.1007/s00344-020-10143-y

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