Abstract
Ajuga xylorrhiza Kit Tan is a rare endemic perennial species growing in the stony habitats in Southeastern Anatolia region, Turkey. An efficient and reproducible protocol has been developed for micropropagation of endangered Ajuga xylorrhiza. Shoots were cultured on Murashige and Skoog (MS) basal medium, supplemented with various concentrations of 6-benzyl amino purine (BAP), thidiazuron (TDZ), and kinetin (Kin). The optimal shoot formation was with the medium containing 0.125 mg L−1 BAP (7.91 shoots per explant), and 0.25 mg L−1 Kin (4.9 shoots per explant) was recorded. The best result in terms of shoot length (3.43 to 3.63 cm) was obtained from the Kin treatments. Kin parameters were more effective on shoot length and multiple shoot production than BAP and TDZ. For rooting, regenerated shoots were transferred to 1/4 strength MS medium containing two different concentrations of indole acetic acid (IAA) and α-naphthalenetic acid (NAA) at 0 (control), 0.5, or 1.0 mg L−1.The best results in terms of the number of roots per shoot were obtained from the medium supplemented with 0.5 mg L−1 IAA (3.10 roots per explant). Rooted plantlets were transferred to soil for acclimization with 100% success rate.
References
Akbaş F, Kuru İS, Karakuş Orcan P (2015) The effects of BAP and Kin on micropropagation of Ajuga vestita BOISS. A rare endemic medicinal plant. 2nd Int' l conference on advances in environment agriculture & medical sciences (ICAEAM'15) June 11-12 Antalya (Turkey)
Akbaş F, Orcan P, Kuru İS, Işıkalan Ç, Namlı S (2019) Optimization of germination protocol for rare endemic Ajuga vestıta Boiss. Under in vitro conditions. Bangladesh J Bot 48:649–653
Anonim (2013) “Ajuga xylorrhiza Tür Koruma Eylem Planı”. TC Orman ve Su İşleri Bakanlığı Doğa Koruma ve Milli Parklar Genel Müdürlüğü XV, Bölge Müdürlüğü Diyarbakır Şube Müdürlüğü Ankara
Buluş Ş (2019) Endemik Ajuga vestita BOISS Bitkisinin Farklı Eksplantlarından İtibaren Kallus Oluşturma Potansiyeli ve Oluşan Kallusun Biyolojik Aktivitesinin Araştırılması. Yüksek Lisans TeziBatman Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı Batman 61 Sayfa
Işık K (2011) Rare and endemic species: why are they prone to extinction? Turk J Bot 35:411–417
Jan M, Singh S, Maqbool F (2018) Micropropagation of Ajuga bracteosa Wall ex Benth-an important medicinal plant growing in Kashmir Himalaya. Int J Adv Res Sci Eng 7:2010–2020
Kaul S, Das S, Srivastava PS (2013) Micropropagation of Ajuga bracteosa a medicinal herb. Physiol Mol Biol Plants 19:289–296
Kayani WK, Fattahi M, Palazon J, Cusido RM, Mirza B (2016) Comprehensive screening of influential factors in the Agrobacterium tumefaciens-mediated transformation of the Himalayan elixir: Ajuga bracteosa. J Appl Res Medicinal Aromatic Plant 3:151–159
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Park HY, Kim DH, Sivanesan I (2017) Micropropagation of Ajuga species: a mini review. Biotechnol Lett 39:1291–1298
Qing X, Yan HM, Ni ZY, Vavricka CJ, Zhang ML, Shi QW, Gu YC, Kiyota H (2017) Chemical and pharmacological research on the plants from genus Ajuga. Heterocycl Commun 23:245–268
Sivanesan I, Ko CH, Lee JP, Jeong BR (2011) Influence of cytokinins on adventitious shoot regeneration from leaf and petiole explants of Ajuga multiflora Bunge. Propag Ornam Plants 11:156–158
Sivanesan I, Park SW (2015) Effect of plant growth regulators on axillary shoot multiplication from nodal explants of A. multiflora Bunge. Propag Ornam Plants 15:42–44
Sivanesan I, Saini RK, Noorzai R, Zamany AJ, Kim DH (2016) In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge. 3. Biotech 6:91–10. https://doi.org/10.1007/s13205-016-0376-z
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The authors would like to thank the Batman University Scientific Research Unit (Project No.: BTÜBAP-2012-FED-11) for the financial support for a part of this study.
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Editor: Marco Buenrostro-Nava
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Işıkalan, Ç., Orcan, P., Akbaş, F. et al. Effect of Cytokinins on In Vitro Propagation of Ajuga xylorrhiza Kit Tan (Critically Endangered), Endemic to Turkey. In Vitro Cell.Dev.Biol.-Plant 56, 911–914 (2020). https://doi.org/10.1007/s11627-020-10082-z
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DOI: https://doi.org/10.1007/s11627-020-10082-z