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The temperature and length for the release of primary and induction of secondary physiological dormancy in Korean pine (Pinus koraiensis Sieb. et Zucc.) seeds

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Abstract

Primary physiological dormancy and secondary physiological dormancy of Korean pine seeds restrict the regeneration of broad-leaved Korean pine (Pinus koraiensis) mixed forest. Dry and imbibed seeds were stratified at 1 °C and 5 °C for 1, 2, 4 and 6 months. Germination percentage, mean germination time (MGT) and germination rate index (GRI) were measured to determine the optimal low temperature and its duration for the release of primary physiological dormancy. Once primary physiological dormancy was released through cold stratification, seeds were stored in an environment in which the temperature progressively increased from 5 to 25 °C. After one month of storage at each storage temperature, the germination percentage, MGT and GRI were measured to determine the threshold temperature for the induction of secondary physiological dormancy. Both dry and imbibed seeds not only exhibited a high germination percentage (approximately 80%) but also germinated rapidly (MGT and GRI were 17 days and 2.36, respectively) after 6 months of storage at either 1 °C or 5 °C. The germination percentage of cold stratified seeds gradually decreased from 78% (5 °C) to 72% (10 °C), 55% (15 °C), 10% (20 °C) and 8% (25 °C). The results of this study suggest that stratifying seeds at 1 °C or 5 °C for 6 months releases primary physiological dormancy. The induction of secondary physiological dormancy occurs at temperatures above 15 °C.

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References

  • Bai Y, Thompson D, Broersma K (2004) Douglas fir and ponderosa pine seed dormancy as regulated by grassland seedbed conditions. J Range Manag 57:661–667

    Article  Google Scholar 

  • Baskin JM, Baskin CC (1998) Seeds: ecology, biogeography, and evolution of dormancy and germination. Academic Press, San Diego

    Google Scholar 

  • Baskin CC, Baskin CC (2004) A classification system for seed dormancy. Seed Sci Res 14:1–16

    Article  Google Scholar 

  • Baskin CC, Baskin JM (2014) Seeds: ecology, biogeography, and evolution of dormancy and germination, 2nd edn. Elsevier/Academic Press, San Diego

    Google Scholar 

  • Benech-Arnold RL, Sánchez RA, Forcella F, Kruk BC, Ghersa CM (2000) Environmental control of dormancy in weed seed banks in soil. Field Crop Res 67:105–122

    Article  Google Scholar 

  • Bewley JD, Black M (1994) Seeds: physiology of development and germination. Plenum Press, New York

    Book  Google Scholar 

  • Bewley JD, Bradford K, Hilhorst H, Nonogaki H (2013) Seeds: physiology of development, germination and dormancy. Springer, New York

    Book  Google Scholar 

  • Brändel M (2004) The role of temperature in the regulation of dormancy and germination of two related summer-annual mudflat species. Aquat Bot 79:15–32

    Article  Google Scholar 

  • Brändel M (2005) The effect of stratification temperatures on the level of dormancy in primary and secondary dormant seeds of two Carex species. Plant Ecol 178:163–169

    Article  Google Scholar 

  • Brändel M, Schütz W (2003) Seasonal dormancy patterns and stratification requirements in seeds of Verbena officinalis L. Basic Appl Ecol 4:329–337

    Article  Google Scholar 

  • Corbineau F, Bianco J, Garello G, Côme D (2002) Breakage of Pseudotsuga menziesii seed dormancy by cold treatment as related to changes in seed ABA sensitivity and ABA levels. Physiol Plant 114:313–319

    Article  CAS  PubMed  Google Scholar 

  • Daneshvar A, Tigabu M, Karimidoost A, Odén PC (2016) Stimulation of germination in dormant seeds of Juniperus polycarpos by stratification and hormone treatments. N For 47:751–761

    Google Scholar 

  • Deng ZJ, Hu XF, Ai XR, Yao L, Deng SM, Pu X, Song SQ (2016) Dormancy release of Cotinus coggygria, seeds under a pre-cold moist stratification: an endogenous abscisic acid/gibberellic acid and comparative proteomic analysis. N For 47:105–118

    Google Scholar 

  • Dey DC, Knapp BO, Battaglia MA, Deal RL, Hart JL, O’Hara KL, Schweitzer CJ, Schuler TM (2019) Barriers to natural regeneration in temperate forests across the USA. N For 50:11–40

    Google Scholar 

  • Einali AR, Sadeghipour HR (2007) Alleviation of dormancy in walnut kernels by moist chilling is independent from storage protein mobilization. Tree Physiol 27:519–525

    Article  CAS  PubMed  Google Scholar 

  • Ellis RA, Roberts EH (1981) The quantification of ageing andsurvival in orthodox seeds. Seed Sci Technol 9:373–409

    Google Scholar 

  • Gosling PG, Samuel Y, Peacea A (2003) The effect of moisture content and prechill durationon dormancy breakage of Douglas fir seeds (Pseudotsuga menziesii var. menziesii [Mirb.] Franco). Seed Sci Res 13:239–246

    Article  Google Scholar 

  • Hawkins TS (2018) Regulating acorn germination and seedling emergence in Quercus pagoda (Raf.) as it relates to natural and artificial regeneration. N For. https://doi.org/10.1007/s11056-018-9667-z

    Article  Google Scholar 

  • Hu XW, Huang XH, Wang YR (2012) Hormonal and temperature regulation of seed dormancy and germination in Leymus chinensis. Plant Growth Regul 67:199–207

    Article  CAS  Google Scholar 

  • Ishikawa Y, Krestov PV, Namikawa K (1999) Disturbance history and tree establishment in old-growth Pinus koraiensis-hardwood forests in the Russian Far East. J Veg Sci 10:439–448

    Article  Google Scholar 

  • Jones SK, Ellis RH, Gosling PG (1997) Loss and induction of conditional dormancy in seeds of Sitka spruce maintained moist at different temperatures. Seed Sci Res 7:351–358

    Article  Google Scholar 

  • Kebreab E, Murdoch A (1999) A quantitative model for loss of primary dormancy and induction of secondary dormancy in imbibed seeds of Orobanche spp. J Exp Bot 50:211–219

    Article  CAS  Google Scholar 

  • Kępczyński J, Bihun M (2002) Induction of secondary dormancy in Amaranthus caudatus seeds. Plant Growth Regu 38:135–140

    Article  Google Scholar 

  • Kildisheva OA, Erickson TE, Kramer AT, Zeldin J, Merritt DJ (2019) Optimizing physiological dormancy break of understudied cold desert perennials to improve seed-based restoration. J Acid Environ. https://doi.org/10.1016/j.jaridenv.2019.104001

    Article  Google Scholar 

  • Koyuncu F (2005) Breaking seed dormancy in black mulberry (Morus nigra L.) by cold stratification and exogenous application of gibberellic acid. Acta Biol Cracov Bot 47:23–26

    Google Scholar 

  • Larsen SU, Eriksen EN (2004) Delayed release of primary dormancy and induction of secondary dormancy in seeds of woody taxa caused by temperature alternations. Acta Hortic 630:91–100

    Article  Google Scholar 

  • Leadem CL (1997) Dormancy-unlocking seed secrets. National Proceedings, Forest and Conservation Nursery Associations. Gen. Tech. Rep. PNW-G TR-419. Portland, OR: US Department of Agriculture, Forest Service, Pacific Northwest Research Station, pp 43–52

  • Li YB, Mou P, Wang TM, Ge J (2012) Evaluation of regeneration potential of Pinus koraiensis in mixed pine-hardwood forests in the Xiao Xing’an Mountains, China. J Forestry Res 23:543–551

    Article  CAS  Google Scholar 

  • Miura K, Araki H (1996) Low temperature treatment during the imbibition period for the induction of secondary dormancy in rice seeds (Oryza sativa L.). Breed Sci 46:235–239

    Google Scholar 

  • Pekrun C, Lutman P, Baeumer K (1997) Induction of secondary dormancy in rape seeds (Brassica napus L.) by prolonged imbibition under conditions of water stress or oxygen deficiency in darkness. Eur J Agron 6:245–255

    Article  Google Scholar 

  • Qi Y, Bilan MV, Chin KL (1993) New method for breaking Korean pine seed dormancy. J Arboric 19:113–117

    Google Scholar 

  • Song Y, Zhu JJ (2016) How does moist cold stratification under field conditions affect the dormancy release of Korean pine seed (Pinus koraiensis)? Seed Sci Technol 44:1–16

    Article  Google Scholar 

  • Song Y, Zhu JJ, Yan QL, Wang GC (2018) Korean pine seed: linking changes in dormancy to germination in the two years following dispersal. Forestry 91:98–109

    Article  Google Scholar 

  • Steadman KJ (2004) Dormancy release during hydrated storage in Lolium rigidum seeds is dependent on temperature, light quality, and hydration status. J Exp Bot 55:929–937

    Article  CAS  PubMed  Google Scholar 

  • Struik GJ (1965) Growth patterns of some native annual and perennial herbs in southern Wisconsin. Ecology 46:401–420

    Article  Google Scholar 

  • Tompsett PB, Pritchard HW (1998) The effect of chilling and moisture status on the germination, desiccation tolerance and longevity of Aesculus hippocastanum L. seed. Ann Bot 82:249–261

    Article  Google Scholar 

  • Wang WQ, Song SQ, Li SH, Gan YY, Wu JH, Cheng HY (2009) Quantitative description of the effect of stratification on dormancy release of grape seeds in response to various temperatures and water contents. J Exp Bot 60:3397–3406

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yamauchi Y, Ogawa M, Kuwahara A, Hanada A, Kamiya Y, Yamaguchi S (2004) Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds. Plant Cell 16:367–378

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yao GQ (1966) The methods to bury seeds of Pinus koraiensis Sieb. et Zucc. and Fraxinus mandshurica for short period. Forest Sci Technol 23:6 (in Chinese)

    Google Scholar 

  • Zhu JJ, Mao ZH, Hu LL, Zhang JX (2007) Plant diversity of secondary forests in response to anthropogenic disturbance levels in montane regions of northeastern China. J Forestry Res 12:403–416

    Article  Google Scholar 

  • Zuo XC (2011) Experience introduction of pine seedling techniques. Forest Inves Des 2:68–69 (in Chinese)

    Google Scholar 

Download references

Acknowledgments

We thank Kai Yang and Lizhong Yu and Xiao Zheng and Tao Sun for their valuable discussion and suggestions about this study. We also thank Hongjun Xu, Jingpu Zhang, Weiwei Zhang and Shuang Xu for their field support and technical assistance. This work was supported by the National Natural Science Foundation of China (31330016).

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Authors and Affiliations

  1. Qingyuan Forest CERN, Chinese Academy of Sciences, Shenyang, 110016, China

    Y. Song, J. J. Zhu & Q. L. Yan

  2. CAS Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Shenyang, 110016, China

    J. J. Zhu & Q. L. Yan

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Y. Song

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  1. Y. Song
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  2. J. J. Zhu
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  3. Q. L. Yan
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Correspondence to J. J. Zhu.

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Song, Y., Zhu, J.J. & Yan, Q.L. The temperature and length for the release of primary and induction of secondary physiological dormancy in Korean pine (Pinus koraiensis Sieb. et Zucc.) seeds. New Forests 51, 657–669 (2020). https://doi.org/10.1007/s11056-019-09750-9

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