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Expression characteristics of immune factors in secondary lymphoid organs of newborn, juvenile and adult yaks (Bos grunniens)

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Abstract

Little is known about lymphoid organ development in yaks. In this study, we characterize and evaluate the main markers of T cell, B cell, plasma cell and antigen-presenting cell in the mesenteric lymph nodes, spleen and hemal node in newborn, juvenile and adult yaks by immunohistochemistry, real-time quantitative polymerase chain reaction and western blotting. The structures of all organs were not fully developed in newborn. The CD3+ cells were mainly located in the paracortex area of the mesenteric lymph node and the T cell dependent area in the hemal node and spleen. CD79a+ cells were mainly detected in the lymphoid follicles. The expression of CD3 and CD79a increased from newborn to juvenile and then decreased in adults. The expression of CD3 was always higher in the spleen and CD79a was higher in the mesenteric lymph node. IgG+ and IgA+ cells were observed in all examined samples, except in newborn yak hemal node. IgG and IgA were up-regulated with age and the highest expression was observed in the mesenteric lymph node. The SIRPα and CD68 were widely expressed. A significant feature was that the SIRPα expression in the spleen was lowest in newborns but highest in juvenile and adult yaks. The expression of CD68 in the hemal node was highest in all groups and increased from newborn to adult yaks. This study sheds light on the relationship between the morphology and function of these organs and provides useful references for normal yak lymphoid organ development.

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Funding

This study was supported by the National Natural Science Foundation of China (Grant No. 31572478).

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Authors and Affiliations

  1. Laboratory of Animal Anatomy & Tissue Embryology, Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China

    Yufeng Huang, Yan Cui, Penggang Liu, Jun Liu, Jun-Feng He & Juan Sun

  2. Gansu Province Livestock Embryo Engineering Research Center, Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China

    Yan Cui & Sijiu Yu

Authors
  1. Yufeng Huang
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  2. Yan Cui
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  3. Sijiu Yu
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  4. Penggang Liu
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  5. Jun Liu
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  6. Jun-Feng He
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  7. Juan Sun
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Correspondence to Yan Cui.

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Ethical approval

All experimental animals were handled according to the Animal Ethics Procedures and Guidelines of the People’s Republic of China and the study was approved by the Institutional Animal Care and Use Committee (IACUC) (No. GSAUAEC-2016-007) of the College of Veterinary Medicine of Gansu Agricultural University.

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Huang, Y., Cui, Y., Yu, S. et al. Expression characteristics of immune factors in secondary lymphoid organs of newborn, juvenile and adult yaks (Bos grunniens). Cell Tissue Res 381, 285–298 (2020). https://doi.org/10.1007/s00441-020-03219-4

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  • DOI: https://doi.org/10.1007/s00441-020-03219-4

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