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Construction of the Heterologous Laccase Producer Aspergillus nidulans lac№4 (argB–) and Its Application for the Progesterone Transformation

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Abstract

A novel Aspergillus nidulans lac№4 (argB–) strain, a producer of recombinant laccase A from basidiomycete Trametes hirsuta 072, was obtained. This strain was used for the biocatalytic transformation of progesterone (PG). The major biotransformation products are 11α-hydroxy-PG, 11α-acetoxy-PG and 6β, 11α-dihydroxy-PG. Macronet MN-200 sorbent was used to study the adsorption kinetics of PG, and the main biotransformation products from the transformation medium. The solid-phase extraction of steroids without preliminary removal of the mycelium was shown to be efficient.

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Funding

This study was supported by the Russian Foundation for Basic Research, project no. 18-34-00653 mol_a.

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Federal Research Center, Fundamentals of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    O. S. Savinova, A. M. Chulkin, E. A. Vavilova, D. V. Vasina & T. V. Fedorova

  2. Faculty of Chemistry, Lomonosov Moscow State University, 119991, Moscow, Russia

    T. S. Savinova

  3. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    P. N. Solyev

Authors
  1. O. S. Savinova
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  2. A. M. Chulkin
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  3. T. S. Savinova
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  4. E. A. Vavilova
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  5. D. V. Vasina
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  6. P. N. Solyev
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  7. T. V. Fedorova
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Corresponding author

Correspondence to O. S. Savinova.

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Translated by A. Boutanaev

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Savinova, O.S., Chulkin, A.M., Savinova, T.S. et al. Construction of the Heterologous Laccase Producer Aspergillus nidulans lac№4 (argB–) and Its Application for the Progesterone Transformation. Appl Biochem Microbiol 56, 321–328 (2020). https://doi.org/10.1134/S0003683820030114

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