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Chloroplast Implication in the Tolerance to Salinity of the Halophyte Cakile maritima

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Abstract

In this study, the response of the chloroplastic antioxidant system of the halophyte Cakile maritima Scop. and its tolerance to NaCl stress have been studied using purified chloroplasts. Seedlings were grown in different salt concentrations (0, 100, 200 and 400 mmol/L NaCl) and plants were harvested after 40 days. Isolated chloroplasts were purified by centrifugation in density-gradients of Percoll. The evaluation of the oxidative stress was analysed measuring lipid peroxidation, carbonyl protein, \({\text{O}}_{2}^{ - }\) and H2O2 contents and the antioxidant status by measurement of the activities of superoxide dismutase, catalase, peroxidase and enzymes of the ascorbate–glutathione cycle as well as the antioxidants ascorbate and glutathione, in the purified chloroplasts. Results revealed that the best growth response of C. maritima under moderate salt stress was associated with a low oxidative stress, the highest activities of SOD, POD and APX and the highest glutathione content. The interaction between the water status of the plant and mineral nutrition seems to be strongly involved in the plant performance under salinity.

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ACKNOWLEDGMENTS

This study was supported by the Tunisian Ministry of Higher Education and Scientific Research and The Spanish Agency of International cooperation for the Development (AECID).

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Authors and Affiliations

  1. Laboratory of Plant Extremophiles, Biotechnology Center, 2050, BorjCédria, Tunisia

    N. Ben Amor & C. Abdelly

  2. Department of Stress Biology and Plant Pathology, CEBAS-CSIC, 30100, Murcia, Spain

    A. Jiménez & F. Sevilla

  3. Laboratory of Molecular Biotechnology of Eukaryotes, CBS, 3018, Sfax, Tunisia

    M. Boudabbous

Authors
  1. N. Ben Amor
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  2. A. Jiménez
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  3. M. Boudabbous
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  4. F. Sevilla
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  5. C. Abdelly
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Correspondence to N. Ben Amor.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants as objects of research.

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Ben Amor, N., Jiménez, A., Boudabbous, M. et al. Chloroplast Implication in the Tolerance to Salinity of the Halophyte Cakile maritima. Russ J Plant Physiol 67, 507–514 (2020). https://doi.org/10.1134/S1021443720030048

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  • DOI: https://doi.org/10.1134/S1021443720030048

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