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CaСl2 Salt Signaling in Primary Root Architecture and Lateral Root Emergence in Arabidopsis thaliana

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Abstract

Plant root architecture modulates during developmental stages and adjusts with the environmental condition. The cytosolic calcium which is a ubiquitous secondary messenger in all eukaryotes strongly affects the root system in Arabidopsis thaliana (L.) Heynh. We proposed that calcium chloride gradients affect PIN2 expression, which in term modulates root architecture and lateral root emergence. In the present study, the root development of PIN2 overexpressing lines of A. thaliana were investigated on different CaCl2 concentrations. This study found that the abundance of PIN2 protein has a direct effect on the root curvature with respect to CaCl2 gradient. In the presence of low concentration of CaCl2, PIN2 protein is stabilized and its subsequent accumulation lead to straight root architecture. However, as the CaCl2 concentration were increased, PIN2 protein destabilized and subsequently degraded which in turn showed a wavy root phenotype. On the other hand, different concentrations of CaCl2 did not show any effect on PIN2 gene at transcript level.

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Authors and Affiliations

  1. Plant Molecular Biology and Biotechnology Center, Division of Applied Life Science (Brain Korea 21 Program), Gyeongsang National University, Jinju City, Korea

    M. Nisar, Z. Ali, A. Ali, R. Aman, H. J. Park & I. Ullah

  2. Department of Botany, University of Malakand, Chakdara, Khyber Pakhtunkhwa, 18550, Dir Lower, Pakistan

    M. Nisar & A. Ullah

  3. Cell Signaling Lab, Konkuk University, Seoul, Korea

    M. Nisar, Z. Ali, A. Ali, R. Aman, H. J. Park, I. Ullah & D. J. Yun

Authors
  1. M. Nisar
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  2. Z. Ali
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  3. A. Ali
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  4. R. Aman
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  5. H. J. Park
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  6. I. Ullah
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  7. A. Ullah
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Contributions

M. Nisar designed the project, conducted experiments and wrote the paper. A. Ullah and H. Park modified and reviewed the paper. Z. Ali, A. Ali, R. Aman and S.F. Wadood helped M. Nisar in the experiments.

Corresponding author

Correspondence to M. Nisar.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants as objects of research.

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Nisar, M., Ali, Z., Ali, A. et al. CaСl2 Salt Signaling in Primary Root Architecture and Lateral Root Emergence in Arabidopsis thaliana. Russ J Plant Physiol 67, 515–520 (2020). https://doi.org/10.1134/S1021443720030176

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  • DOI: https://doi.org/10.1134/S1021443720030176

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