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In Vivo Assessment of the Effect of Hexagonal Boron Nitride Nanoparticles on Biochemical, Histopathological, Oxidant and Antioxidant Status

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Abstract

The aim of our study is to investigate the dose-dependent biological system effect of hexagonal boron nitride (hBN) nanoparticles, which is directly produced nanoscale, in vivo. Wistar albino rats (n = 80) weighing 200–250 g were divided into eight groups (n = 10). The acute effects of hBN NPs (i.v) on the rats were investigated by measuring the biochemical, hematological parameters and oxidant-antioxidant status. The results show that no significant change was observed in the hematological and biochemical parameters when the control group and other low dose groups were compared, except for the 1600 and 3200 µg/kg b.w. dose groups. Histological detection indicated that 1600 and 3200 µg/kg hBN NPs treatment could induce significant damage in the liver, kidney, heart, spleen and pancreas. With the findings obtained, it can be seen that hBN NPs cannot be evaluated independently of particle morphology, and that the hBN NPs used in this study may be suitable for biomedical applications where low doses between 50 and 800 µg/kg are not toxic.

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Acknowledgements

This study was supported by Eskişehir Technical University Scientific Research Projects Commission. (Project No: 19ADP163). The authors would like to thank BORTEK Boron Technologies and Mechatronic Inc. for their material support and Dr. Umut SAVACI for his help to use Transmission Electron Microscopy (TEM) (Eskisehir Technical University, Turkey).

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey

    Fatih Kar & Güngör Kanbak

  2. Department of Medical Biochemistry, Faculty of Medicine, Duzce University, Duzce, Turkey

    Ceyhan Hacıoğlu

  3. Department of Biomedical Engineering, Faculty of Engineering and Architecture, Osmangazi University, Eskisehir, Turkey

    Yapıncak Göncü

  4. Boron Technologies and Mechatronics Inc, Eskisehir, Turkey

    Yapıncak Göncü & Nuran Ay

  5. Department of Medical Biochemistry, Faculty of Medicine, Demiroglu Bilim University, İstanbul, Turkey

    İbrahim Söğüt

  6. Department of Biology, Faculty of Arts and Sciences, Eskisehir Osmangazi University, Eskisehir, Turkey

    Hakan Şenturk

  7. Department of Histology and Embryology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey

    Dilek Burukoğlu Dönmez

  8. Department of Materials Science and Engineering, Eskisehir Technical University, Eskisehir, Turkey

    Nuran Ay

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  1. Fatih Kar
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Correspondence to Fatih Kar.

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Ethics Approval

The study was approved by the Committee of Local Ethics Committee of Eskişehir Osmangazi University Animal Experiments (ESOGU HADYEK) (Decision No: 662-1 of 20.02.2019).

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Kar, F., Hacıoğlu, C., Göncü, Y. et al. In Vivo Assessment of the Effect of Hexagonal Boron Nitride Nanoparticles on Biochemical, Histopathological, Oxidant and Antioxidant Status. J Clust Sci 32, 517–529 (2021). https://doi.org/10.1007/s10876-020-01811-w

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