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Production of 2-keto-gluconic acid from glucose by immobilized Pseudomonas plecoglossicida resting cells

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Abstract

2-Keto-d-gluconic acid (2KGA) is an important organic acid derived from d-glucose and is used to produce the food antioxidant erythorbic acid. To improve the 2KGA production performance and cell reusability, various carriers such as calcium alginate, k-carrageenan, chitosan, and poly(vinyl alcohol)-alginate were evaluated to immobilize Pseudomonas plecoglossicida JUIM01 resting cells. Calcium alginate was shown to be a suitable carrier since the immobilized cells had the highest number of reuse times and produced the highest 2KGA concentration of 171.77 g/L, with a productivity of 3.58 g/L·h and conversion ratio of 98.38%. The cell concentration, cultivation temperature, aeration rate and initial glucose concentration were further optimized in a 5-L airlift bioreactor to obtain the best 2KGA production performance by calcium alginate-immobilized P. plecoglossicida cells. Under the optimal conditions including a cell concentration of 4.0 g/L, glucose concentration of 126.0 g/L, temperature of 34 °C and aeration rate of 2.8 L/min, 134.45 g/L 2KGA was produced by alginate-immobilized P. plecoglossicida cells within 30 h, with a total productivity of 4.48 g/L·h and yield of 1.07 g/g (conversion ratio of over 99.0%). The immobilized cells maintained a stable conversion capacity after nine reuses and 25 days of storage at 4 °C, which indicated that calcium alginate immobilization of P. plecoglossicida cells had industrial practicability for 2KGA production.

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Funding

This work was funded by the Natural Science Foundation of China (31571885), Science and Technology Platform Construction Program of Jiangxi Province (2010DTZ01900), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Author notes

  1. Zhiliang Hou and Lei Sun are the co-first authors and have the equal contributions.

Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People’s Republic of China

    Zhiliang Hou, Daming Wang, Wenjing Sun & Fengjie Cui

  2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Lei Sun

  3. Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-Production, Dexing, 334221, People’s Republic of China

    Wenjing Sun, Fengjie Cui & Silian Yu

Authors
  1. Zhiliang Hou
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  2. Lei Sun
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  3. Daming Wang
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  4. Wenjing Sun
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  5. Fengjie Cui
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  6. Silian Yu
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Contributions

W.-J.S. conceived of the study, participated in its design and coordination, and drafted the manuscript. Z.-L.H and L.S performed experiments, analyzed data, and helped to draft the manuscript. D.-M.W., F.-J.C and S.-L.Y performed partial experiments and analyzed data. All authors read and approved the manuscript.

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Correspondence to Wenjing Sun.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Hou, Z., Sun, L., Wang, D. et al. Production of 2-keto-gluconic acid from glucose by immobilized Pseudomonas plecoglossicida resting cells. 3 Biotech 10, 253 (2020). https://doi.org/10.1007/s13205-020-02243-z

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  • DOI: https://doi.org/10.1007/s13205-020-02243-z

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