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miR-1224-5p inhibits the proliferation and invasion of ovarian cancer via targeting SND1

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Abstract

Emerging evidences have indicated that abnormal expression of microRNAs (miRNAs) contributed to carcinogenesis of ovarian cancer. However, the molecular mechanism of many aberrant expressed miRNAs was not known. Here, we discovered that miR-1224-5p was a downregulated miRNA in ovarian cancer via bioinformatic analysis and RT-qPCR. It was found that upregulation of miR-1224-5p inhibited cell proliferation and invasion ability of ovarian cancer cells. SND1, a well-characterized oncogene, was predicted as a target gene of miR-1224-5p. The western blotting, dual luciferase reporter assay, RNA-binding protein immunoprecipitation assay, and RT-qPCR demonstrated SND1 as a target gene of miR-1224-5p in ovarian cancer. MiR-1224-5p inhibited the expression of mesenchymal markers and increased the expression of epithelial markers in ovarian cancer cells via targeting SND1, indicating miR-1224-5p was involved in epithelial mesenchymal transition. The rescue assay manifested that miR-1224-5p-regulated cell proliferation and invasion mainly rely on downregulation of SND1 in ovarian cancer cells. In conclusion, our study revealed a direct regulatory association between miR-1224-5p and SND1 and their involvement in ovarian carcinogenesis.

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Author notes

  1. Junrong Wang and Yubo Hu contributed equally to this study.

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, China-Japan Union Hospital of Jilin University, Changchun, 130033, Jilin, People’s Republic of China

    Junrong Wang, Cong Ye & Junbao Liu

  2. Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, 130033, Jilin, People’s Republic of China

    Yubo Hu

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  1. Junrong Wang
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  2. Yubo Hu
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  4. Junbao Liu
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Contributions

JW, YH, and CY performed the experiments and analyzed the data. JW and JL collected the specimens and the information of patients. CYe and JL wrote the manuscript.

Corresponding author

Correspondence to Junbao Liu.

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The authors declare that they have no competing interests, and all authors confirmed its accuracy.

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The protocol of the present study was approved by the ethic committee of China-Japan Union Hospital of Jilin University.

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Wang, J., Hu, Y., Ye, C. et al. miR-1224-5p inhibits the proliferation and invasion of ovarian cancer via targeting SND1. Human Cell 33, 780–789 (2020). https://doi.org/10.1007/s13577-020-00364-4

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  • DOI: https://doi.org/10.1007/s13577-020-00364-4

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