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A potent protective effect of baicalein on liver injury by regulating mitochondria-related apoptosis

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A Correction to this article was published on 18 July 2020

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Abstract

Liver injury is the early stage of liver disease, which is caused by multiple factors. Baicalein has shown extensive bioactivity. But whether baicalein has a protective effect on liver injury has not been reported thus far. In this study, we aim to investigate the protective effects of baicalein on liver injury induced by oxidative stress. H2O2 and CCl4 were employed to establish liver injury models in vivo and in vitro, respectively. The protective effect of baicalein on oxidative stress–induced liver injury was evaluated by detecting the mitochondrial dynamics, the level of autophagy and apoptosis, the histopathology of liver, the indicators of liver function, and the level of oxidative stress in vitro and in vivo. March5 is the key regulator during liver injury induced by oxidative stress. March5 can ubiquitinate Drp1 and promote Drp1 degradation, then maintain the homeostasis of mitochondrial dynamics, keep the balance of autophagy, and reduce apoptosis. Baicalein is able to effectively reduce liver injury; it can contribute to the expression of March5 by regulating KLF4 during liver injury. These results indicate that baicalein plays a key role in salvaging liver from injury induced by oxidative stress via regulating the KLF4-March5-Drp1 signal pathway.

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  • 18 July 2020

    The original version of this article unfortunately contains errors in methods and figures. In methods, animal experiments part: ���2��ml/kg��� and ���after two days��� were described incorrectly which were actually ���4��ml/kg��� and ���after eight days��� respectively.

Abbreviations

March5:

Membrane-associated RING-CH 5

ROS:

Reactive oxygen species

Drp1:

Dynamin related protein 1

KLF4:

Kruppel-like factor 4

H2DCFDA:

2′,7′-Dichlorofluorescin-diacetate

8-OHG:

8-Hydroxyguanosine

PARP:

Poly (ADP‑ribose) polymerase

Bcl:

B‑cell lymphoma‑2

BAX:

Bcl-2 associated X protein

LC3-I:

Microtubule associated protein 1 light chain 3 I

LC3-II:

Microtubule associated protein 1 light chain 3 II

SiRNA:

Small interfering RNA

ALT:

Alanine aminotransferase

AST:

Aspartate aminotransferase

DBIL:

Direct bilirubin

TBIL:

Total bilirubin

SOD:

Superoxide dismutase

CAT:

Catalase; GSH-PX, glutathione peroxidase

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Acknowledgements

This work was supported by the National Science Foundation of China (grant numbers 81741173; 31430041; 31671447; 91849209).

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Authors and Affiliations

  1. Institute for Translational Medicine, Qingdao University, #38 Dengzhou Road, Qingdao, 266000, Shandong, People’s Republic of China

    Zhongjie Yu, Yin Wang & Peifeng Li

  2. School of Basic Medicine, Qingdao University, Qingdao, People’s Republic of China

    Zhongjie Yu

  3. Departments of Emergency Medicine, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, People’s Republic of China

    Qi Li

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  1. Zhongjie Yu
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ZY, YW and PL generated the idea, edited the manuscript; ZY and QL performed the experiments and analyzed the data; ZY, QL prepared the manuscript; PL provided the funding.

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Correspondence to Yin Wang or Peifeng Li.

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This article contained studies with mice. All experiments were performed according to the protocols approved by the Institutional Animal Care and Use Committee of Affiliated Hospital of Qingdao University.

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Yu, Z., Li, Q., Wang, Y. et al. A potent protective effect of baicalein on liver injury by regulating mitochondria-related apoptosis. Apoptosis 25, 412–425 (2020). https://doi.org/10.1007/s10495-020-01608-2

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