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Identification and expression analysis of pathogenicity-related genes in Tilletia indica inciting Karnal bunt of wheat

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Abstract

Wheat (Triticum aestivum L.) is a major cereal crop cultivated all over the world. Karnal bunt disease is an internationally quarantined disease caused by Tilletia indica. In modern resistance breeding, effectors have been efficiently used to identify, functionally characterize and deploy resistance genes. The present study was carried out to identify the genes related to pathogenicity in T. indica by mining whole genome sequence and transcriptome data. The secretory proteins were identified using bioinformatics tools. In total, 1337 unique proteins having secretory signatures were predicted in T. indica genome. Out of 192 genes related to host-pathogen interaction (PHI database), 7 pathogenicity-related genes were selected for relative expression analysis at different time points under in vitro and in planta conditions using qPCR. Under in vitro, maximum expression of genes was up to 3 fold on amendment with spike tissue extract from susceptible host. Under in planta, three genes (Ti2035, Ti2347 and Ti3774) showed the maximum expression at 3 days post inoculation (dpi). Further two genes (Ti57 and Ti198) showed the maximum up-regulation at 3 dpi followed by 10 dpi and 15 dpi. Two genes (Ti10340 and Ti12741) were up regulated at later stages at 10 dpi and 15 dpi only in susceptible genotype. Ti2035, Ti2347 and Ti3774 genes might have role in penetration, Ti57 and Ti198 genes may be related to penetration & infection, Ti10340 and Ti12741 genes may have role in sporulation when co-related with earlier reported histo-pathological studies. This study could be utilized to understand the mechanism(s) of pathogenesis through functional genomics for developing strategies for management of Karnal bunt of wheat.

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References

  • Aggarwal R, Singh DV, Srivastava KD (1994) Host-pathogen interaction in Karnal bunt of wheat. Indian Phytopathol 47(4):381–385

    Google Scholar 

  • Aggarwal R, Singh DV, Srivastava KD (1999) Studies on the ontogeny of teliospore ornamentation of Neovossia indica observed through scanning electron microscopy. Indian Phytopathol 52(4):417–419

    Google Scholar 

  • Aggarwal R, Tripathi A, Yadav A (2010) Pathogenic and genetic variability in Tilletia indica monosporidial culture lines using universal rice primer-PCR. Eur J Plant Pathol 128(3):333–342

    Article  Google Scholar 

  • Aujla SS, Sharma I, Singh BB (1987) Physiologic specialization of Karnal bunt of wheat. Indian Phytopathol 40:333–336

    Google Scholar 

  • Bansal R, Singh DV, Joshi LM (1983) Germination of teliospores of Karnal bunt of wheat. Seed Res 11:258

    Google Scholar 

  • Bencina M, Panneman H, Ruijter GJ, Legisa M, Visser J (1997) Characterization and overexpression of the Aspergillus niger gene encoding the cAMP-dependent protein kinase catalytic subunit. Microbiology 143:1211–1220

    Article  CAS  Google Scholar 

  • Bonde MR, Berner DK, Nester SE, Peterson GL, Olsen MW, Cunfer BM, Sim T (2004) Survival of Tilletia indica teliospores in different soils. Plant Dis 88:316–324

    Article  CAS  Google Scholar 

  • Chomczynski P, Mackey K (1995) Short technical report. Modification of the TRIZOL reagent procedure for the isolation of RNA from polysaccharide-and proteoglycan-rich sources. Biotechniques 19(6):942–945

    CAS  PubMed  Google Scholar 

  • Conesa A, Gotz S (2008) Blast2GO: a comprehensive suite for functional analysis in plant genomics. Int J Plant Genomics 2008:1–12. https://doi.org/10.1155/2008/619832

    Article  CAS  Google Scholar 

  • Dyer RB, Plattner RD, Kendra DF, Brown DW (2005) Fusarium graminearum TRI14 is required for high virulence and DON production on wheat but not for DON synthesis in vitro. J Agric Food Chem 53(23):9281–9287

    Article  CAS  Google Scholar 

  • FAO (2018) Food and Agriculture Organization of the United Nations [Internet]. Available from: http://www.fao.org/faostat/en/#data/QC [Accessed: April 25, 2020]

  • Gill KS, Indu S, Aujla SS (1993) Karnal bunt and wheat production. Punjab Agricultural University, Ludhiana, p 153

    Google Scholar 

  • Gurjar MS, Aggarwal R, Jogawat A, Kulshreshtha D, Sharma S, Solanke AU, Dubey H, Jain RK (2019) De novo genome sequencing and secretome analysis of Tilletia indica inciting Karnal bunt of wheat provides pathogenesis-related genes. 3 Biotech 9(6):219

    Article  PubMed Central  Google Scholar 

  • Haas BJ, Kamoun S, Zody MC, Jiang RH, Handsaker RE (2009) Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans. Nature 461:393–398

    Article  CAS  Google Scholar 

  • Hardham AR, Blackman LM (2018) Phytophthora cinnamomi. Mol Plant Pathol 19(2):260–285

    Article  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25(4):402–408

    Article  CAS  Google Scholar 

  • Mitra M (1931) A new bunt on wheat in India. Ann Appl Biol 18(2):178–179

    Article  Google Scholar 

  • MoA&FW (2018–19) Ministry of Agriculture and Farmers Welfare, Government of India [Internet]. Available from: https://eands.dacnet.nic.in/PDF/Pocket%20Book%202019.pdf [Accessed: April 25, 2020]

  • Munjal RL, Chatrath MS (1976) Studies on the mode of infection of Neovossia indica incitant of Karnal bunt of wheat. J Nucl Agric Biol 5(2):40–41

    Google Scholar 

  • Nagarajan S, Aujla SS, Nanda GS, Sharma I, Goel LB, Kumar J, Singh DV (1997) Karnal bunt (Tilletia indica) of wheat–a review. Rev Plant Pathol 76(12):1207–1214

    Google Scholar 

  • Raffaele S, Farrer RA, Cano LM, Studholme DJ, MacLean D, Thines M, Jiang RH, Zody MC, Kunjeti SG, Donofrio NM, Meyers BC (2010) Genome evolution following host jumps in the Irish potato famine pathogen lineage. Science 330:1540–1543

    Article  CAS  Google Scholar 

  • Shretha B, Blondeau K, Stevens WF, Hegarat FL (2004) Expression of chitin deacetylase from Colletotrichum lindemuthianum in Pichia pastoris: purification and characterization. Protein Expr Purif 38(2):196–204

    Article  Google Scholar 

  • Singh DV, Gogoi R (2011) Karnal bunt of wheat (Triticum spp) a global scenario. Indian J Agric Sci 81(1):3–14

    Google Scholar 

  • Singh J, Aggarwal R, Gurjar MS, Sharma S, Saharan MS (2018) Characterisation of isolates of Tilletia indica inciting Karnal bunt of wheat (Triticum aestivum L.). Int J Curr Microbiol App Sci 7(12):xx-xx. https://doi.org/10.20546/ijcmas.2018.712.xx

    Article  Google Scholar 

  • Singh J, Aggarwal R, Gurjar MS, Sharma S, Saharan MS (2019) Identification of carbohydrate active enzymes from the whole genome sequence of Tilletia indica and sporulation analysis. Indian J Agric Sci 89(6):1023–1026

    CAS  Google Scholar 

  • Spanu PD, Abbott JC, Amselem J, Burgis TA, Soanes DM (2010) Genome expansion and gene loss in powdery mildew fungi reveal tradeoffs in extreme parasitism. Science 330:1543–1546

    Article  CAS  Google Scholar 

  • Tripathi A, Aggarwal R, Yadav A (2013) Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat. Turk J Biol 37(5):606–613

    Article  CAS  Google Scholar 

  • Tyler BM, Tripathy S, Zhang X, Dehal P, Jiang RH (2006) Phytophthora genome sequences uncover evolutionary origins and mechanisms of pathogenesis. Science 313:1261–1266

    Article  CAS  Google Scholar 

  • Underwood W (2012) The plant cell wall: a dynamic barrier against pathogen invasion. Front Plant Sci 3:85. https://doi.org/10.3389/fpls.2012.00085

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Verma S, Gazara RK, Nizam S, Parween S, Chattopadhyay D, Verma PK (2016) Draft genome sequencing and secretome analysis of fungal phytopathogen Ascochyta rabiei provides insight into the necrotrophic effector repertoire. Sci Rep 6:24638

    Article  CAS  PubMed Central  Google Scholar 

  • Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14(6):415–421

    Article  Google Scholar 

  • Zhao Z, Liu H, Wang C, Xu J (2013) Comparative analysis of fungal genomes reveals different plant cell wall degrading capacity in fungi. BMC Genomics 23(14):274–289

    Article  CAS  Google Scholar 

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Acknowledgements

The authors gratefully acknowledge the financial support received from the Indian Council of Agricultural Research, New Delhi in the form of CRP genomics project (ICAR-G/CRP-Genomics/2015-2720/IARI-12-151) for this research work. We are thankful to PG School, ICAR-IARI for providing fellowship to the first author. We are also highly thankful to Director, Joint Director (Research), and Head, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi for providing guidance and facilities to carry out this study.

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Correspondence to R. Aggarwal.

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Singh, J., Aggarwal, R., Gurjar, M.S. et al. Identification and expression analysis of pathogenicity-related genes in Tilletia indica inciting Karnal bunt of wheat. Australasian Plant Pathol. 49, 393–402 (2020). https://doi.org/10.1007/s13313-020-00711-x

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  • DOI: https://doi.org/10.1007/s13313-020-00711-x

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