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Evolutionary and recombination analysis of porcine reproductive and respiratory syndrome isolates in China

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Abstract

Seven strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated from 2014 to 2017 in the Shandong province of China and their genomes were sequenced and analyzed. Results showed that all seven of the isolates belong to PRRSV 2, and are clustered into four lineages (lineage 1, 3, 5 and 8) based on comparisons of the ORF5 gene. Comparative analysis of genomes and specific amino acid sites revealed that three of the strains (SDwh1402, SDwh1602 and SDwh1701) have evolved directly from modified live virus (MLV) JXA1-P80, TJM-F92 and IngelvacPRRS. Further recombination analysis revealed that two of the strains (SDyt1401 and SDwh1601) were the result of a recombination event between MLVs JXA1-P80 and NADC30 while two other strains (SDwh1403 and SDqd1501) were the result of recombination between MLVs IngelvacPRRS and NADC30 and HP-PRRSV and QYYZ, respectively. Our results add to the data on MLV evolution and PRRSV recombination and provide a better understanding of the epidemiology of PRRSV in China.

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  • 27 June 2020

    The original version of this article unfortunately contained an error in GenBank Accession Number.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (31502016) and TECH-BANK CO., LTD.

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Author notes

  1. Zhendong Zhang and Xiangyang Qu have contributed equally to this work.

Authors and Affiliations

  1. School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212018, China

    Zhendong Zhang, Xudong Tang, Ting Bian & Ping Wu

  2. TECH-BANK CO., LTD, Shanghai, 200233, China

    Xiangyang Qu, Yingjun Sun, Mingming Zhou & Fubo Ren

  3. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China

    Hongliang Zhang

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  1. Zhendong Zhang
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Contributions

ZZ and XQ interpreted the data and drafted the work. PW coordinated the study. HZ, XT and TB confirmed the analyses of recombination events. YS, MZ and FR contributed to provide the background information of isolates. All authors read and approved the final manuscript.

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Correspondence to Ping Wu.

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Zhang, Z., Qu, X., Zhang, H. et al. Evolutionary and recombination analysis of porcine reproductive and respiratory syndrome isolates in China. Virus Genes 56, 354–360 (2020). https://doi.org/10.1007/s11262-020-01751-7

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