Abstract
Novel duck reovirus (NDRV), the prototype strain of the species Avian orthoreovirus (ARV), is associated with high mortality in Pekin ducklings. σC is an outer capsid protein encoded by the S1 genome segment of NDRV which mediates attachment to host cells. Our previous studies using immunoprecipitation and mass spectrometry found that σC coprecipitated with some host proteins including Translocation-associated membrane protein 1 (TRAM1). However, the interaction between σC and TRAM1 has not been further confirmed experimentally. In this study, we utilized coimmunoprecipitation assays, glutathione S-transferase pull-down, and confocal microscopy to confirm the interaction between σC and TRAM1. In addition, knockdown of TRAM1 using siRNA and overexpression of TRAM1 gene were conducted to explore its effect on virus replication. The result showed that TRAM1 silencing benefits while overexpression inhibits viral replication. This study confirms the important role TRAM1 during NDRV infection which can help develop new approaches for NDRV disease prevention and control.
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Acknowledgements
This work was supported by grants from the national key research and development plan for controlling of major diseases of livestock and poultry (Nos. 2017YFD0500802, 2016YFD0500802), the National Nature Science Foundation of China (Nos. 31502068, U1703117), the Natural Science Foundation of Shanghai (No. 19ZR1468800).
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ZC, HZ and RX designed the experiments; RX, XM, JS, MD, JZ, CL and WG carried out the experiments; ZC, HZ, GL and RX analyzed the data; RX wrote the first draft; ZC and GL reviewed and edited the manuscript.
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Xiao, R., Mi, X., Sun, J. et al. Interaction between Translocation-associated membrane protein 1 and σC protein of novel duck reovirus controls virus infectivity. Virus Genes 56, 347–353 (2020). https://doi.org/10.1007/s11262-020-01750-8
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DOI: https://doi.org/10.1007/s11262-020-01750-8