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The Effect of KcsA Channel on Lipid Bilayer Electroporation Induced by Picosecond Pulse Trains

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Abstract

Membrane proteins are the major component of plasma membranes, and they play crucial roles in all organisms. To understand the influence of the presence of KcsA channel on cell membrane electroporation induced by picosecond pulse trains (psPT), in this paper, the electroporation of KcsA membrane protein system and bare lipid bilayer system (POPC) with the applied psPT are simulated using molecular dynamics (MD) method. First, we find that the average pore formation time of the KcsA system is longer than the bare system with the applied psPT. In the KcsA system, water protrusions appear more slowly. Then, the system size effects of psPT in the MD simulations are investigated. When the system size decreases, the average pore formation time of small KcsA membrane protein system is shorter than the bare system with the applied psPT. It is found that the psPT makes the protein fluctuation of small system increase greatly; meanwhile the instability of protein disturbs the water and then affects the water protrusion appearance time. Furthermore, it shows that the protein fluctuation of constant electric field is smaller than that of psPT and no field, and protein fluctuation increases with the psPT repetition frequency increasing.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 61921002).

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Authors and Affiliations

  1. School of Electronic Science and Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan, China

    Jingchao Tang, Jialu Ma, Lianghao Guo, Kaicheng Wang, Yang Yang, Wenfei Bo, Zhe Wu, Baoqing Zeng & Yubin Gong

  2. CNRS, UMR 7565, 54506, Vandoeuvre les Nancy, France

    Jingchao Tang

  3. School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, Singapore

    Lixia Yang

  4. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, China

    Haibo Jiang

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  1. Jingchao Tang
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  2. Jialu Ma
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  5. Yang Yang
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Correspondence to Yubin Gong.

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Tang, J., Ma, J., Guo, L. et al. The Effect of KcsA Channel on Lipid Bilayer Electroporation Induced by Picosecond Pulse Trains. J Membrane Biol 253, 271–286 (2020). https://doi.org/10.1007/s00232-020-00123-4

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  • DOI: https://doi.org/10.1007/s00232-020-00123-4

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