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Distribution Patterns of nirS-Encoding and nirK-Encoding Denitrifiers in the Surface Sediment of the Pearl River Estuary

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Abstract

The abundance and community structure of nirS-encoding and nirK-encoding denitrifiers inhabiting the sediment of the Pearl River Estuary was examined with quantitative PCR and high-throughput MiSeq sequencing. The results indicated that the low-oxygen condition (around 4 mg/L) of the Pearl River Estuary was the suitable environment for the denitrifying bacterial growth, and the abundances of nirS-encoding and nirK-encoding denitrifier were both highest in station P3, while the diversity and evenness were highest in stations P2 and P4, respectively. In addition, gene abundance and diversity of nirS was higher than nirK, which indicated that the denitrifying potential of nirS-encoding denitrifiers were significantly greater than that of nirK-encoding denitrifiers (p < 0.05). The most dominant nirS-encoding denitrifiers present in the sediment samples belonged to the phylum Proteobacteria, followed by Chloroflexi. However, the dominant classes of Betaproteobacteria and Gammaproteobacteria showed obvious salinity heterogeneity along the Pearl River Estuary. Betaproteobacteria have a strong survival ability in oligohaline environments and Gammaproteobacteria were opposite. Additionally, the distribution of both Betaproteobacteria and Gammaproteobacteria positively correlated with TOC and \({\text{NO}}_{2}^{ - }.\) For nirK-encoding denitrifiers, the distribution of cluster A and cluster C also showed salinity heterogeneity; the former favored oligohaline environments and the latter were opposite.

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Funding

This work was supported by the National Key Research and Development Program of China (grant nos. 2018YFD0900704 and 2018YFD0900703), the National Natural Science Foundation of China (grant no. 31900094), Central Public-interest Scientific Institution Basal Research Fund (CAFS, grant no. 2019ZD0102), Financial Fund of the Ministry of Agriculture, P.R. China (no. NFZX2018), 2018 Special Project for Economic Development of Guangdong (grant no. GDME-2018B001).

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  1. Guangdong Provincial Key Laboratory of Fishery Ecology and Environment and Key Laboratory of Open-Sea Fishery Development, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, 510300, Guangzhou, China

    Rongjun Shi, Honghui Huang, Zhanhui Qi & Tingting Han

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Correspondence to Zhanhui Qi.

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Shi, R., Huang, H., Qi, Z. et al. Distribution Patterns of nirS-Encoding and nirK-Encoding Denitrifiers in the Surface Sediment of the Pearl River Estuary. Russ J Mar Biol 45, 453–463 (2019). https://doi.org/10.1134/S1063074019060099

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