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Assessment of proliferation, migration and differentiation potentials of bone marrow mesenchymal stem cells labeling with silica-coated and amine-modified superparamagnetic iron oxide nanoparticles

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Abstract

Superparamagnetic iron oxide nanoparticles have been widely used for cell labeling in preclinical and clinical studies, to improve labeling efficiency, particle conjugation and surface modifications are developed, but some modified SPIONs exert side-effect on physiological activity of cells, which cannot be served as ideal cell tracker. In this study, amine-modified silica-coated SPIO (SPIO@SiO2-NH2, SPIO@S-N) nanoparticles were used to label bone marrow derived mesenchymal stem cells (BM-MSCs), then the stem cell potentials were evaluated. It was found BM-MSCs could be efficiently labeled by SPIO@S-N nanoparticles. After labeling, the BM-MSCs viability kept well and the migration ability increased, but the osteogenesis and adipogenesis potentials were not impaired. In steroid associated osteonecrosis (SAON) bone defect model, stem cell implantation was performed by injection of SPIO@S-N labeled BM-MSCs into marrow cavity locally, it was found the SPIO positive cells homed to the periphery of defect region in control group, but were recruited to the defect region in poly lactic-coglycolic acid/tricalcium phosphate (PLGA/TCP) scaffold implantation group. In conclusion, SPIO@S-N nanoparticles promoted migration while retained proliferation and differentiation ability of BM-MSCs, implying this kind of nanoparticles could be served not only an ideal tracking marker but also an accelerator for stem cell homing during tissue repair.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (81560005 and 81760007), Natural Science Foundation of Guangxi province (2016GXNSFBA380003 and 2018GXNSFAA138052).

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Authors and Affiliations

  1. The Laboratory of Respiratory Disease, The Affiliated Hospital of Guilin Medical University, Guilin, 541000, China

    Dong Yao

  2. Guangxi Colleges and Universities Key Laboratory of Respiratory Disease, Guilin, 541000, China

    Dong Yao & Bi-wen Mo

  3. Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Guilin Medical University, Guilin, 541000, China

    Dong Yao

  4. Department of Basic Medical Sciences, School of Nursing, Sanmenxia Polytechnic, Sanmenxia, 472000, China

    Na-na Liu

  5. The Second Affiliated Hospital of Guilin Medical University, Guilin, 541100, China

    Bi-wen Mo

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  1. Dong Yao
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Correspondence to Dong Yao or Bi-wen Mo.

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Yao, D., Liu, Nn. & Mo, Bw. Assessment of proliferation, migration and differentiation potentials of bone marrow mesenchymal stem cells labeling with silica-coated and amine-modified superparamagnetic iron oxide nanoparticles. Cytotechnology 72, 513–525 (2020). https://doi.org/10.1007/s10616-020-00397-5

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