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Isolation and expression analysis of two novel C-repeat binding factor (CBF) genes involved in plant growth and abiotic stress response in mangrove Kandelia obovata

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Abstract

Kandelia obovata is one of the cold tolerant mangrove plants along the China coast. To reveal the cold tolerant mechanism of K. obovata, the present work isolated two CBF/DREB1 genes (designated KoCBF1 and KoCBF3) from cold-stressed K. obovata and characterized their expression profiles in various organs and in response to multiple abiotic stresses. The deduced proteins of KoCBF1 and 3 all contain specific features of CBFs, and show high similarity to AmCBF1 and 3 from Avicennia marina, respectively. Different expression patterns of the two CBF orthologous under various abiotic stresses and exogenous hormone suggested that they may have different regulators and be involved in different regulatory pathway. The high basal and cold induced expression of the two genes indicated that they may all play important roles in growth and cold resistance of plants. The significant induction of KoCBF3 after salt and lead (Pb2+) treatments suggested that this CBF gene may also participate in response to salinity and heavy metal stresses. This study will provide a better understanding of CBF-regulated stress-resistant mechanism, which may be benefit in mangrove biotechnological breeding, high-latitude transplanting, and bioremediation of heavy metal pollutions.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (No.U1901211, No.41430966 and No.41876126), the National Key Research and Development Plan (No.2017FY100700), the International Partnership Program of Chinese Academy of Sciences (No.133244KYSB20180012) and the Strategic Priority Research Program of the Chinese Academy of Sciences (No.XDA23050200, No.XDA13010500 and No. XDA13020503).

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Authors and Affiliations

  1. State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Ya-Lan Peng, You-Shao Wang, Jiao Fei & Cui-Ci Sun

  2. Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen, 518121, China

    You-Shao Wang & Cui-Ci Sun

  3. Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, 510301, China

    You-Shao Wang, Jiao Fei & Cui-Ci Sun

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  1. Ya-Lan Peng
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  2. You-Shao Wang
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  4. Cui-Ci Sun
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Correspondence to You-Shao Wang.

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Peng, YL., Wang, YS., Fei, J. et al. Isolation and expression analysis of two novel C-repeat binding factor (CBF) genes involved in plant growth and abiotic stress response in mangrove Kandelia obovata. Ecotoxicology 29, 718–725 (2020). https://doi.org/10.1007/s10646-020-02219-y

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