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Efficient synthesis of deuterium-labelled Danshensu for quantitative bioanalysis

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Abstract

Danshensu (DSS) is a bioactive compound isolated from the Chinese herb Radix Salviae Miltiorrhizae, which has been applied for the treatment of angina pectoris. The stable isotope-labelled DSS is required to be used for clinical studies and biological sample analysis. This study aims to synthesize deuterium-labelled DSS to change the status of DSS without internal standard, and to accelerate the research progress of DSS in vivo. An effective and convenient method for the synthesis of deuterium-labelled DSS was developed using Pd/C as a catalyst and D2O as a deuterium source. Our results showed that deuterium-labelled DSS with high deuterium incorporation has been achieved and could be used as internal standards in stable isotopic dilution MS/MS studies to accurately quantify DSS levels in biologic samples.

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Acknowledgements

The work is supported by the Shanghai Municipal Health and Family Planning Commission (20184Y0033), the Shanghai Science and Technology Committee (18401933300), and Important Weak Discipline Construction Project of the Pudong New Area Health System (PWZbr2017-17).

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Author notes

  1. Yi Li and Honglei Xu have contributed equally to this work.

Authors and Affiliations

  1. Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China

    Yi Li & Min Chen

  2. School of Pharmacy, Second Military Medical University, Shanghai, 200433, China

    Honglei Xu, Kun He, Chengzhong Zhang & Chuan Zhang

  3. Medical School, Shanghai University, Shanghai, 200433, China

    Yi Li & Liyan Xiong

  4. School of Medicine, Shanghai University, Shanghai, 200444, China

    Tingfang Wang & Chuan Zhang

  5. Department of Pharmacy, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, 201318, China

    Min Chen

  6. The 983th Hospital of Joint Logistics Support Forces of Chinese PLA, Tianjin, 300142, China

    Honglei Xu

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  1. Yi Li
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  2. Honglei Xu
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  3. Kun He
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  5. Chengzhong Zhang
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  6. Tingfang Wang
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  7. Chuan Zhang
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  8. Min Chen
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Correspondence to Tingfang Wang, Chuan Zhang or Min Chen.

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Li, Y., Xu, H., He, K. et al. Efficient synthesis of deuterium-labelled Danshensu for quantitative bioanalysis. J Radioanal Nucl Chem 325, 167–173 (2020). https://doi.org/10.1007/s10967-020-07190-w

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  • DOI: https://doi.org/10.1007/s10967-020-07190-w

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