Abstract
This study focused on the relevance between the carotid plaque formation and the single nucleotide polymorphisms of chromosome 9p21 and CD147 in acute non-cardiogenic cerebral infarction. A total of 937 eligible patients were enrolled and categorized into carotid plaque group or non-carotid plaque group. The baseline data was analyzed, and the SNPs of chromosome 9p21 and CD147 were detected. After analyzing the results of clinic data and SNPs, we found that age, total cholesterol, low density lipoprotein, systolic blood pressure, fasting serum glucose, and NIHSS score are associated with plaque formation. Meanwhile, rs10757274, rs4977574, and rs4919862 existed statistical differences between two groups. We also analyzed linkage disequilibrium, haplotype, and inheritance models of these three SNPs, and drew the ROC curve to assess diagnostic efficiency. The results showed that mutations of SNP rs10757274 and rs4977574 in chromosome 9p21 together with SNP rs4919862 located in gene CD147 were highly relevant with the carotid plague formation in acute cerebral ischemia patients. Compared with single SNP genotype mutation, combined allele mutations on rs10757274 or rs4977574 in chromosome 9p21 with rs4919862 in CD147 resulted in much higher risks of patients, which might be associated with acute cerebral infarction happening.
Similar content being viewed by others
References
Bi J, Yang L, Liu D, Wu J, Tong X, Cen S, Zhou D, Zhang T, Yi L (2015) Sequence variants on chromosome 9p21 are associated with ischemic stroke and the lipids level in Chinese Han population. J Stroke Cerebrovasc Dis 24(4):894–900
Cunnington MS, Santibanez Koref M, Mayosi BM, Burn J, Keavney B (2010) Chromosome 9p21 SNPs associated with multiple disease phenotypes correlate with ANRIL expression. PLoS Genet 6(4):e1000899
Fornage M, Doris PA (2005) Single-nucleotide polymorphism genotyping for disease association studies. Methods Mol Med 108:159–172
Jin R, Xiao AY, Chen R, Granger DN, Li G (2017) Inhibition of CD147 (cluster of differentiation 147) ameliorates acute ischemic stroke in mice by reducing thromboinflammation. Stroke 48(12):3356–3365
Kaur H, Prakash A, Medhi B (2013) Drug therapy in stroke: from preclinical to clinical studies. Pharmacology 92(5–6):324–334
Khoshnam SE, Winlow W, Farzaneh M, Farbood Y, Moghaddam HF (2017) Pathogenic mechanisms following ischemic stroke. Neurol Sci 38(7):1167–1186
Kim AS et al (2015) Global Stroke Belt: geographic variation in stroke burden worldwide. Stroke 46(12):3564–3570
Lu Z et al (2015) Variants on chromosome 9p21 confer risks of noncardioembolic cerebral infarction and carotid plaque in the Chinese Han population. J Atheroscler Thromb 22(10):1061–1070
McPherson R et al (2007) A common allele on chromosome 9 associated with coronary heart disease. Science 316(5830):1488–1491
Meens MJ et al (2012) Risky communication in atherosclerosis and thrombus formation. Swiss Med Wkly 142:w13553
Ni X, Zhang J (2014) Association between 9p21 genomic markers and ischemic stroke risk: evidence based on 21 studies. PLoS One 9(3):e90255
Ni T, Chen M, Yang K, Shao J, Fu Y, Zhou W (2017) Association of CD147 genetic polymorphisms with carotid atherosclerotic plaques in a Han Chinese population with cerebral infarction. Thromb Res 156:29–35
Picher AJ, Hernández F, Budeus B, Soriano E, Avila J (2018) Human brain single nucleotide polymorphism: validation of DNA sequencing. J Alzheimers Dis Rep 2(1):103–109
Pott J, Burkhardt R, Beutner F, Horn K, Teren A, Kirsten H, Holdt LM, Schuler G, Teupser D, Loeffler M, Thiery J, Scholz M (2017) Genome-wide meta-analysis identifies novel loci of plaque burden in carotid artery. Atherosclerosis 259:32–40
Ritz K et al (2014) Cause and mechanisms of intracranial atherosclerosis. Circulation 130(16):1407–1414
Su H, Yang Y (2018) The roles of CyPA and CD147 in cardiac remodelling. Exp Mol Pathol 104(3):222–226
Sun H, Zou X, Liu L (2013) Epidemiological factors of stroke: a survey of the current status in China. J Stroke 15(2):109–114
Sutherland BA et al (2012) Neuroprotection for ischaemic stroke: translation from the bench to the bedside. Int J Stroke 7(5):407–418
von Ungern-SternbergSNI, et al. (2018). "Extracellular matrix metalloproteinase inducer EMMPRIN (CD147) in cardiovascular disease." Int J Mol Sci 19(2)
Xu B et al (2018) Study of serum CD147 level in patients with transient ischemic attack and CD147 expression in atherosclerotic plaque. J Cardiovasc Transl Res 11(4):285–291
Yue X, Tian L, Fan X, Xu G, Shi FD, Liu X (2015) Chromosome 9p21.3 variants are associated with cerebral infarction in Chinese population. J Mol Neurosci 56(3):546–552
Zhang W et al (2012) Variants on chromosome 9p21.3 correlated with ANRIL expression contribute to stroke risk and recurrence in a large prospective stroke population. Stroke 43(1):14–21
Zhou J, Song B, Duan X, Long Y, Lu J, Li Z, Zeng S, Zhan Q, Yuan M, Yang Q, Xia J (2014) Association of BSG genetic polymorphisms with atherosclerotic cerebral infarction in the Han Chinese population. Int J Neurosci 124(10):734–740
Funding
This work was sponsored by the National Nature Science Foundation of China (81771281).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
This study was approved by the Ethics Committee of Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine.
Conflict of Interest
The authors declare that they have no conflicts of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Jin, W., Wu, W., Yang, K. et al. The Single Nucleotide Polymorphisms of Chromosome 9p21 and CD147 Were Relevant with the Carotid Plaque Risk in Acute Cerebral Infarction Patients Among Chinese Han Population. J Mol Neurosci 70, 1282–1292 (2020). https://doi.org/10.1007/s12031-020-01540-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12031-020-01540-9