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Rice CaM-binding transcription factor (OsCBT) mediates defense signaling via transcriptional reprogramming

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Abstract

The mutant allele of rice calmodulin-binding transcription activator OsCBT, oscbt-1, exhibits broad-spectrum resistance against rice pathogens. Previously, we reported that the strong resistance of the oscbt-1 mutant to pathogens was conferred by a constitutive upregulation of defense-related genes even under pathogen-free conditions. We also found strong induction of the hypersensitive response as a reaction to pathogen invasion. The results suggest that OsCBT acts as a negative regulator of basal resistance to pathogen attack. To identify the transcriptional network regulated by OsCBT, we compared global gene expression profiles between wild-type (WT) and oscbt-1 rice plants grown under pathogen-free conditions. The results of a 3′ tiling microarray revealed that in oscbt-1 plants, 81 genes are upregulated and 200 genes are downregulated when compared with the WT. A gene ontology analysis showed that differentially regulated genes in oscbt-1 were very closely associated with “death” GO term in a biological process category, and “catalytic activity” and “binding” GO terms in a molecular function category. A MapMan analysis indicated that the functions of these genes were associated with plant responses to biotic stress. Moreover, the results from quantitative real-time PCR in the oscbt-1 mutant showed a significant effect on the gene expression patterns of the fungal pathogen response. Our results suggested that the OsCBT regulates a rice defense response by modulating the expressions of various defense-related genes.

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Acknowledgements

This work was supported by Next Generation BioGreen21 Program (SSAC, Grant Number: PJ01318202), Rural Development Administration Republic of Korea and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2013R1A1A2062074 to M.C.K and 2016R1D1A1B01011803 to D.B.).

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Author notes

  1. Jung-Sung Chung, Sung Cheol Koo, Byung Jun Jin, and Dongwon Baek have contributed equally to the work.

Authors and Affiliations

  1. Department of Agronomy, Gyeongsang National University, Jinju, 52828, Korea

    Jung-Sung Chung, Sang In Shim, Jong-Il Chung & Min Chul Kim

  2. Division of Applied Life Science (BK21 Plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 52828, Korea

    Sung Cheol Koo, Byung Jun Jin, Dongwon Baek, Seon-In Yeom, Man Soo Choi, Hyun Min Cho, Su Hyeon Lee, Wook-Hun Jung, Cheol Woo Choi & Min Chul Kim

  3. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Korea

    Hyun Jin Chun & Min Chul Kim

  4. Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, 17104, Korea

    Anil Kumar Nalini Chandran & Ki-Hong Jung

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  1. Jung-Sung Chung
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Correspondence to Ki-Hong Jung or Min Chul Kim.

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Chung, JS., Koo, S.C., Jin, B.J. et al. Rice CaM-binding transcription factor (OsCBT) mediates defense signaling via transcriptional reprogramming. Plant Biotechnol Rep 14, 309–321 (2020). https://doi.org/10.1007/s11816-020-00603-y

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