Abstract
Plasmid pCAMBIA2201-pta, containing the selectable marker neomycin phosphotransferase gene (npt II), Pinellia ternata agglutinin (PTA) gene and β-glucuronidase gene, were transformed into leaf discs of Chrysanthemum (Chrysanthemum morifolium Ramat.) via Agrobacterium tumefaciens-mediated transformation. It was confirmed by GUS (β-glucuronidase) and PCR analysis that the PTA gene had been successfully inserted into the plant genome. Hemagglutination assay was carried out and the expression of PTA gene in transgenic plants in vitro and the aphid-resistance assay result showed that the inhibition rates of the transformed plants were improved than the control. T1 progenies generated through the cuttage breeding from T0 generation had preserved the aphid-resistance and the inhibition rates of T1 generation had no significant difference with T0 generation (P > 0.05). The results suggest that PTA gene can improve aphid resistance of chrysanthemum and its aphid resistance can be generated to T1 generation stably.
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References
Davies FT, He CJ, Chau A, Heinz KM, Cartmill AD (2004) Fertility affects susceptibility of chrysanthemum to cotton aphids: influence on plant growth, photosynthesis, ethylene evolution, and herbivore abundance. J Am Soc Hortic Sci 129:344–353
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21. https://doi.org/10.1007/BF02712670
Duan X, Hou Q, Liu G, Pang X, Niu Z, Wang X, Zhang Y, Li B, Liang R (2018) Expression of Pinellia pedatisecta lectin gene in transgenic wheat enhances resistance to wheat aphids. Molecules 23:748
Fu X, Su J, Yu K, Cai Y, Zhang F, Chen S, Fang W, Fadi C, Guan Z (2018) Genetic variation and association mapping of aphid (Macrosiphoniella sanbourni) resistance in chrysanthemum (Chrysanthemum morifolium Ramat.). Euphytica 214:21. https://doi.org/10.1007/s10681-017-2085-z
Fukai S, De Jong J, Rademaker W (1995) Agrobacterium-mediated genetic transformation of chrysanthemum. Acta Hort 392:147–152. https://doi.org/10.17660/actahortic.1995.392.17
Gatehouse AMR, Down RE, Powell KS, Sauvion N, Rahbe Y, Newell CA, Merryweather A, Hamilton WDO, Gatehouse JA (1996) Transgenic potato plants with enhanced resistance to the peach-potato aphid Myzus persicae. Entomol Exp Appl 79:295–307. https://doi.org/10.1111/j.1570-7458.1996.tb00837.x
Hilder VA, Powell KS, Amr G, Gatehouse JA, Gatehouse LN, Shi Y, Wdo H, Merryweather A, Newell CA, Timans JC (1995) Expression of snowdrop lectin in transgenic tobacco plants results in added protection against aphids. Transgenic Res 4:18–25. https://doi.org/10.1007/BF01976497
Ho TS, Pak HS, Ryom CK, Han MH (2019) Overexpression of OsmiR393a gene confers drought tolerance in creeping bentgrass. Plant Biotechnol Rep. https://doi.org/10.1007/s11816-019-00517-4
Jin S, Zhang X, Daniell H (2012) Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, Lepidopteran insects, bacterial and viral pathogens. Plant Biotechnol J 10:313–327. https://doi.org/10.1111/j.1467-7652.2011.00663.x
Jun TH, Mian MAR, Michel AP (2013) Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324. Heredity 111:16–22. https://doi.org/10.1038/hdy.2013.10
Li P, Song A, Gao C, Jiang J, Chen S, Fang W, Zhang F, Chen F (2015) The over-expression of a chrysanthemum WRKY transcription factor enhances aphid resistance. Plant Physiol Biochem 95:26–34. https://doi.org/10.1016/j.plaphy.2015.07.002
Ling LJ, Yang YZ, Bi YR (2010) Expression and characterization of two domains of Pinellia ternata agglutinin (PTA), a plant agglutinin from Pinellia ternata with antifungal activity. World J Microbiol Biotechnol 26:545–554. https://doi.org/10.1007/s11274-009-0204-2
Maqbool SB, Husnain T, Riazuddin S, Masson L, Christou P (1998) Effective control of yellow stem borer and rice leaf folder in transgenic rice indica varieties Basmati 370 and M 7 using the novel delta-endotoxin cry2A Bacillus thuringiensis gene. Mol Breed 4:501–507. https://doi.org/10.1023/a:1009660315970
Nagadhara D, Ramesh S, Pasalu IC, Rao YK, Krishnaiah NV, Sarma NP, Bown DP, Gatehouse JA, Reddy VD, Rao KV (2003) Transgenic indica rice resistant to sap-sucking insects. Plant Biotechnol J 1:231–240. https://doi.org/10.1046/j.1467-7652.2003.00022.x
Naing AH, Trinh Ngoc A, Jeon SM, Lim SH, Kim CK (2016) An efficient protocol for Agrobacterium-mediated genetic transformation of recalcitrant chrysanthemum cultivar Shinma. Acta Physiol Plant. https://doi.org/10.1007/s11738-015-2059-5
Nishikawa Y (2005) method of manufacturing ammonium sulfate crystal. JP Patent JP 2005194153 A, 2005/07/21
Powell KS, Gatehouse AMR, Hilder VA, Gatehouse JA (1993) Antimetabolic effects of plant lectins and plant fungal enzymes on the nymphal stages of two important rice pests, Nilaparvata lugens and Nephotettix cincticeps. Entomol Exp Appl 66:119–126. https://doi.org/10.1111/j.1570-7458.1993.tb00699.x
Rao KV, Rathore KS, Hodges TK, Fu X, Stoger E, Sudhakar D, Williams S, Christou P, Bharathi M, Bown DP, Powell KS, Spence J, Gatehouse AMR, Gatehouse JA (1998) Expression of snowdrop lectin (GNA) in transgenic rice plants confers resistance to rice brown planthopper. Plant J 15:469–477. https://doi.org/10.1046/j.1365-313X.1998.00226.x
Rao KK, Hodges TK, Fu X, Stoger E, Sudhakar D, Williams S, Christou P, Bharathi M (2010) Expression of snowdrop lectin (GNA) in transgenic rice plants confers resistance to rice brown planthopper. Plant J 15:469–477. https://doi.org/10.1046/j.1365-313X.1998.00226.x
Shih TT, Lee HL, Chen SC, Kang CY, Shen RS, Su YA (2018) Rapid analysis of traditional Chinese medicine Pinellia ternata by microchip electrophoresis with electrochemical detection. J Sep Sci 41:740–746. https://doi.org/10.1002/jssc.201700901
Shinoyama H, Komano M, Nomura Y, Nagai T (2002) Introduction of delta-endotoxin gene of Bacillus thuringiensis to Chrysanthemum [Dendranthema × grandiflorum (Ramat.) Kitamura] for insect resistance. Breed Sci 52:43–50. https://doi.org/10.1270/jsbbs.52.43
Sjahril R, Jamaluddin I, Nadir M, Asman, Dungga NE, Iop (2018) Effect of selection agents to Chrysanthemum (Chrysanthemum morifolium) callus growth after Agrobacterium-mediated genetic transformation. In: 1st International conference on food security and sustainable agriculture in the tropics (IC-FSSAT), Hasanuddin Univ, Fac Agr & Publicat Management Ctr, Makassar, Indonesia, Oct 24–25 2017. IOP conference series-earth and environmental science. Iop Publishing Ltd, BRISTOL. https://doi.org/10.1088/1755-1315/157/1/012044
Sota T, Kagata H, Ando Y, Utsumi S, Osono T (2014) Insect–plant interactions in plant-based community/ecosystem genetics. Species diversity and community structure. Springer, Tokyo, pp 25–43. https://doi.org/10.1007/978-4-431-54261-2_2
Stoger E, Williams S, Christou P, Down RE, Gatehouse JA (1999) Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid Sitobion avenae. Mol Breed 5:65–73. https://doi.org/10.1023/a:1009616413886
Tang K, Sun X, Hu Q, Wu A, Lin CH, Lin HJ, Twyman RM, Christou P, Feng T (2001) Transgenic rice plants expressing the ferredoxin-like protein (AP1) from sweet pepper show enhanced resistance to Xanthomonas oryzae pv. oryzae. Plant Sci 160:1035–1042. https://doi.org/10.1016/s0168-9452(01)00351-x
Valizadeh M, Kazemitaba SK, Jongsma MA (2012) Agrobacterium-mediated genetic transformation of chrysanthemum (Chrysanthemum morifolium Ramat.) with an aphidicidal gene, gcs (gamma-cadinene synthase). Int J Plant Breed Genet 6:168–181. https://doi.org/10.3923/ijpbg.2012.168.181
Van Damme EJM (2008) Plant lectins as part of the plant defense system against insects. In: Schaller A (ed) Induced plant resistance to herbivory. Springer, Dordrecht, pp 285–307. https://doi.org/10.1007/978-1-4020-8182-8_14
Visser PB, Maagd RAd, Jongsma MA (2007) III.3 Chrysanthemum. In: Pua EC, Davey MR (eds) Transgenic crops VI. Biotechnology in agriculture and forestry, vol 61. Springer, Berlin, pp 253–272
Wang GL, Liu YH, Guo SH, Wang Y, Ji Y, Fang HJ (2004) Study on transformation of snowdrop lectin gene to chrysanthemum and aphid resistance of the transgenic plants. Yi chuan xue bao Acta Genet Sin 31:1434–1438
Wang C, Fei Z, Guan Z, Chen S, Jiang J, Fang W, Chen F (2014) Inheritance and molecular markers for aphid (Macrosiphoniella sanbourni) resistance in chrysanthemum (Chrysanthemum morifolium Ramat.). Sci Hortic 180:220–226. https://doi.org/10.1016/j.scienta.2014.10.038
Wei Z, Yue H, Shaowei X, Yong G, Wenduo C, Miao D, Zhili Y, Tao X (2013) Prokaryotic expression and bioactivity analysis of N-terminus domain of Pinellia ternata agglutinin using alkaline phosphatase signal peptide. Protein Exp Purif 89:84–91. https://doi.org/10.1016/j.pep.2013.03.001
Wu J, Luo X, Guo H, Xiao J, Tian Y (2006) Transgenic cotton, expressing Amaranthus caudatus agglutinin, confers enhanced resistance to aphids. Plant Breed 125:390–394. https://doi.org/10.1111/j.1439-0523.2006.01247.x
Yao JH, Pang YZ, Qi HB, Zhao XY, Kong WW, Sun XF, Tang KX (2003) Transgenic tobacco expressing Pinellia ternata agglutinin confers enhanced resistance to aphids. Transgen Res 12:715–722. https://doi.org/10.1023/B:TRAG.0000005146.05655.7d
Yuan ZQ, Zhao CY, Zhou Y, Tian YC (2001) Aphid-resistant transgenic tobacco plants expressing modified gna gene. Acta Botan Sin 43:592–597
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This study was supported by the DPR of Korea State Committee for Scientific Research.
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Pak, S., Han, M., Li, H. et al. Breeding of the transgenic chrysanthemum (Chrysanthemum morifolium Ramat.) carrying aphid-resistance gene, Pinellia ternata agglutinin (PTA). Plant Biotechnol Rep 14, 255–262 (2020). https://doi.org/10.1007/s11816-019-00587-4
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DOI: https://doi.org/10.1007/s11816-019-00587-4