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Stimulatory effects of short-term calcitonin administration on plasma calcium, magnesium, phosphate, and glucose in juvenile Siberian sturgeon Acipenser baerii

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Abstract

Calcitonin (CT) has a potential function in calcium (Ca) regulation, but there are conflicting observations in fishes. Because of the lack of calcified endoskeleton, sturgeons have low Ca circulating compared with teleost fish and the function of CT on Ca in sturgeon is very less understood. The purpose of this study was to investigate the impact of injection of salmon CT on plasma Ca, magnesium (Mg), phosphate (PHO), and glucose levels of juvenile Siberian sturgeon Acipenser baerii. Sixteen-month-old fish (429.6 ± 12.1 g) were intraperitoneally injected with a single dose of CT (5 μg kg−1 BW) and saline solution as a control group. Thereafter, blood sampling was performed at 0, 1, 2, 4, 6, 12, 24, and 48 h after injection. CT produced marked increases in all variables measured. The highest levels of Ca (6.77 ± 0.53 mg dL−1), Mg (9.79 ± 0.16 mg dL−1) and PHO (1.74 ± 0.05 mg dL−1) were recorded at 2 h after CT injection and showed significant difference compared with control treatment (Ca 4.75 ± 0.12 mg dL−1; Mg 5.47 ± 0.16 mg dL−1 and PHO 1.23 ± 0.06 mg dL−1). It also likely produced hyperglycemia. However, the differences with the controls were not statistically significant, possibly due to interference with the hyperglycemia induced by the stress of injection. Our results showed that the injection of 5 μg kg−1 BW CT to Siberian sturgeon has an incremental effect on plasma Ca, Mg, and PHO. The increase in plasma Ca level indicated that CT has a potent hypercalcemic effect in sturgeon under laboratory condition, in contrast to the hypocalcemic effects reported for teleosts.

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References

  • Allen PJ, Webb MA, Cureton E, Bruch RM, Barth CC, Peake SJ, Anderson WG (2009) Calcium regulation in wild populations of a freshwater cartilaginous fish, the lake sturgeon Acipenser fulvescens. Comp Biochem Physiol 154A:437–450

    Article  CAS  Google Scholar 

  • Berndt TJ, Schiavi S, Kumar R (2005) “Phosphatonins” and the regulation of phosphorus homeostasis. Am J Physiol Renal Physiol 289:1170–1182

    Article  Google Scholar 

  • Björnsson BT, Deftos LJ (1985) Plasma calcium and calcitonin in the marine teleost, Gadus morhua. Comp Biochem Physiol 81A:593–596

    Article  Google Scholar 

  • Björnsson BT, Young G, Lin RJ, Deftos LJ, Bern HA (1989) Smoltification and seawater adaptation in coho salmon (Oncorhynchus kisutch): plasma calcium regulation, osmoregulation, and calcitonin. Gen Comp Endocrinol 74:346–354

    Article  Google Scholar 

  • Butakova SS, Nozdrachev AD (2011) Mechanisms of hyperglycemic effect of calcitonin. Bull Exp Biol Med 150:320–323

    Article  CAS  Google Scholar 

  • Candill SP, Boone DJ (1986) Analytical variance and definition of a reference change as a function of calcium concentration. Clin Chem 32:308–313

    Article  Google Scholar 

  • Chakrabarti P, Mukherjee D (1993) Studies on the hypocalcemic actions of salmon calcitonin and ultimobranchial gland extracts in the freshwater teleost Cyprinus carpio. Gen Comp Endocrinol 90:267–273

    Article  CAS  Google Scholar 

  • Chan DKO, Jones IC, Smith RN (1968) The effect of mammalian calcitonin on the plasma levels of calcium and inorganic phosphate in the European eel (Anguilla anguilla L.). Gen Comp Endocrinol 11:243–245

    Article  CAS  Google Scholar 

  • Copp DH, Byfield PGHM, Kerr RC, Newsome F, Walker V, Watts EG (1972) Calcitonin and ultimobranchial function in fishes and birds. In: Talmage RV, Munson PL (eds) Calcitonin, parathyroid hormone, and the Calcitonins. Excerpta Medica Foundation, Amsterdam, pp 12–20

    Google Scholar 

  • de Rouffignac C, Quamme G (1994) Renal magnesium handling and its hormonal control. Physiol Rev 74:305–322

    Article  Google Scholar 

  • Di Stefano A, Elalouf JM, Garel JM, de Rouffignac C (1985) Modulation by calcitonin of magnesium and calcium urinary excretion in the rat. Kidney Int 27:394–400

    Article  Google Scholar 

  • Ehrhardt V, Appel W, Paschen K (1992) Evaluierung eines Xylidyl-Blau-Reagenz zur Bestimmung von Magnesium. Wien Klin Wochenschr 104:5–11

  • Fenwick JC, Lam TJ (1988) Effects of calcitonin on plasma calcium and phosphate in the mudskipper, Periophthalmodon schlosseri (Teleostei), in water and during exposure to air. Gen Comp Endocrinol 70:224–230

  • Fouchereau-Peron M, Arlot-Bonnemains Y, Moukhtar MS, Milhaud G (1986) Adaptation of rainbow trout (Salmo gairdnerii) to sea water: changes in calcitonin levels. Comp Biochem Physiol 83A:83–87

  • Fouchereau-Peron M, Arlot-Bonnemains Y, Moukhtar MS, Milhaud G (1987) Calcitonin induces hypocalcaemia in grey mullet and immature fresh water and sea water adapted rainbow trout. Comp Biochem Physiol 87A:1051–1053

    Article  CAS  Google Scholar 

  • Fuentes J, Haond C, Guerreiro PM, Silva N, Power DM, Canário AV (2007) Regulation of calcium balance in the sturgeon Acipenser naccarii: a role for PTHrP. Am J Phys Regul Integr Comp Phys 293:R884–R893

    CAS  Google Scholar 

  • Glowacki J, O’sullivan J, Miller M, Wilkie DW, Deftos LJ (1985) Calcitonin produces hypercalcemia in leopard sharks. Endocrinology 116:827–829

    Article  CAS  Google Scholar 

  • Henry RJ, Cannon DC, Winkelman JW (1974) Clinical chemistry: principles and techniques, 2nd edn. Harperand Row Publisher, Hagerstown, pp 287–341

    Google Scholar 

  • Hirano T, Hasegawa S, Yamauchi H, Orimo H (1981) Further studies on the absence of hypocalcemic effects of eel calcitonin in the eel, Anguilla japonica. Gen Comp Endocrinol 43:42–50

    Article  CAS  Google Scholar 

  • Hirsch PF, Baruch H (2003) Is calcitonin an important physiological substance? Endocrine 21:201–208

    Article  CAS  Google Scholar 

  • Jafari N, Falahatkar B (2019) Growth performance and physiological changes of holding juvenile Siberian sturgeon at different sizes in rearing units. Aquac Res 50:699–706

    Article  CAS  Google Scholar 

  • Jafari N, Falahatkar B, Sajjadi MM (2018) Growth performance and plasma metabolites in juvenile Siberian sturgeon Acipenser baerii (Brandt, 1869) subjected to various feeding strategies at different sizes. Fish Physiol Biochem 44:1363–1374

    Article  CAS  Google Scholar 

  • Knowles S, Hrubec TC, Smith SA, Bakal RS (2006) Hematology and plasma chemistry reference intervals for cultured shortnose sturgeon (Acipenser brevirostrum). Vet Clin Pathol 35:434–440

    Article  Google Scholar 

  • Lafont AG, Wang YF, Chen GD, Liao BK, Tseng YC, Huang CJ, Hwang PP (2011) Involvement of calcitonin and its receptor in the control of calcium-regulating genes and calcium homeostasis in zebrafish (Danio rerio). J Bone Miner Res 26:1072–1083

    Article  CAS  Google Scholar 

  • Lopez E, Peignoux-Deville J, Lallier F, Martelly E, Milet C (1976) Effects of calcitonin and ultimobranchialectomy (UBX) on calcium and bone metabolism in the eel, Anguilla anguilla L. Calcif Tissue Res 20:173–186

    Article  CAS  Google Scholar 

  • Lott JA, Turner K (1975) Evaluation of Trinder’s glucose oxidase method for measuring glucose in serum and urine. Clin Chem 21:1754–1760

    Article  CAS  Google Scholar 

  • Moisa SS (2017) Calcitonin participant in the development of insulin resistance. J Biomed Sci Eng 10:343–354

    Article  CAS  Google Scholar 

  • Mukherjee D, Sen U, Bhattacharyya SP, Mukherjee D (2004) The effects of calcitonin on plasma calcium levels and bone metabolism in the fresh water teleost Channa punctatus. Comp Biochem Physiol 138A:417–426

    Article  CAS  Google Scholar 

  • Oughterson SM, Munoz-Chapuli R, De Andres V, Lawson R, Heath S, Davies DH (1995) The effects of calcitonin on serum calcium levels in immature brown trout, Salmo trutta. Gen Comp Endocrinol 97:42–48

    Article  CAS  Google Scholar 

  • Passariello N, Giugliano D, Sgambato S, Torella R, D’onofrio F (1981) Calcitonin, a diabetogenic hormone? J Clin Endocrinol Metab 53:318–323

    Article  CAS  Google Scholar 

  • Renfro JL (1997) Hormonal regulation of renal inorganic phosphate transport in the winter flounder, Pleuronectes americanus. Fish Physiol Biochem 17:377–383

    Article  CAS  Google Scholar 

  • Sasayama Y (1999) Hormonal control of Ca homeostasis in lower vertebrates: considering the evolution. Zool Sci 16:857–869

    Article  CAS  Google Scholar 

  • Srivastav AK, Srivastav SK, Sasayama Y, Suzuki N (1998) Salmon calcitonin induced hypocalcemia and hyperphosphatemia in an elasmobranch, Dasyatis akajei. Gen Comp Endocrinol 109:8–12

    Article  CAS  Google Scholar 

  • Urist ME, Van de Putte KA (1967) Comparative biochemistry of the blood of fishes. In: Gilbert PW, Mathenson MW, Hall DP (eds) Sharks, skates, and rays. Johns Hopkins Press, Baltimore, pp 271–285

    Google Scholar 

  • Wales NAM, Barrett AL (1983) Depression of sodium, chloride and calcium ions in the plasma of goldfish (Carassius auratus) and immature freshwater-and seawater-adapted eels (Anguilla anguilla L.) after acute administration of salmon calcitonin. J Endocrinol 98:257–261

    Article  CAS  Google Scholar 

  • Wales NAM, Gaunt T (1987) Blood pressure, electrolyte composition and renal studies of pike, Esox lucius, following administration of salmon calcitonin. J Fish Biol 30:659–665

    Article  CAS  Google Scholar 

  • Wendelaar Bonga SE (1980) Effect of synthetic salmon calcitonin and low ambient calcium on plasma calcium, ultimobranchial cells, Stannius bodies, and prolactin cells in the teleost Gasterosteus aculeatus. Gen Comp Endocrinol 40:99–108

    Article  CAS  Google Scholar 

  • Wendelaar Bonga SE, Pang PKT (1991) Control of calcium regulating hormones in the vertebrates: parathyroid hormone, calcitonin, prolactin, and stanniocalcin. Int Rev Cytol 128:139–213

    Article  CAS  Google Scholar 

  • Yamauchi H, Orimo H, Yamauchi K, Takano K, Takahashi H (1978) Increased calcitonin levels during ovarian development in the eel, Anguilla japonica. Gen Comp Endocrinol 36:526–529

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank the staff at the Faculty of Natural Resources, University of Guilan for their assistance. We are also thankful to H.A. Zamani and Dr. M. Hadavi who supported us with their valuable help during the analysis.

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Authors and Affiliations

  1. Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, 1144, Iran

    Naghmeh Jafari, Hamed Abdollahpour & Bahram Falahatkar

  2. Department of Marine Sciences, The Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran

    Bahram Falahatkar

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  1. Naghmeh Jafari
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  2. Hamed Abdollahpour
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  3. Bahram Falahatkar
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Correspondence to Bahram Falahatkar.

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Jafari, N., Abdollahpour, H. & Falahatkar, B. Stimulatory effects of short-term calcitonin administration on plasma calcium, magnesium, phosphate, and glucose in juvenile Siberian sturgeon Acipenser baerii. Fish Physiol Biochem 46, 1443–1449 (2020). https://doi.org/10.1007/s10695-020-00801-z

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