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A human cell polarity protein Lgl2 regulates influenza A virus nucleoprotein exportation from nucleus in MDCK cells

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Abstract

In this study, the regulatory effect of the overexpression of polarity protein Lgl2 on the nuclear export of influenza A virus nucleoprotein in infected cells was investigated. A stable Tet-Off inducible MDCK cell line expressing a fusion protein comprising Lgl2 and an enhanced yellow fluorescent protein were used. TCID50 analysis and neuraminidase activity analysis revealed that replication of influenza A virus was inhibited in Lgl2 overexpressing cells. By immunofluorescence microscopical observation at different time point post virus infection, a retention of NP in cellular nucleus was found in Lgl2 overexpressing cells. Compared with normal MDCK cells, change in claudin-1 distribution between cell contacts caused by Lgl2 overexpression impaired the barrier function of tight junction. These results suggest that changes in cell polarity induced by Lgl2 overexpressing will affect virus NP transportation.

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Acknowledgements

We thank Dr. Quansheng Du (Medical College of Georgia, Augusta University, USA), for technical support.

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Authors and Affiliations

  1. School of Pharmaceutical Science, Wuhan University, 185 Donghu Road, Wuhan, 430071, Hubei, China

    Jing Liu, Mengdan Fang, Xuexin Chen & Xiaobo Zeng

  2. College of Life Science, South-Center University for Nationalities, Wuhan, 430074, Hubei, China

    Haiying Wang

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  1. Jing Liu
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  2. Haiying Wang
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  3. Mengdan Fang
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  4. Xuexin Chen
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  5. Xiaobo Zeng
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Correspondence to Xiaobo Zeng.

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Corresponding editor: Kundan sengupta

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Liu, J., Wang, H., Fang, M. et al. A human cell polarity protein Lgl2 regulates influenza A virus nucleoprotein exportation from nucleus in MDCK cells. J Biosci 45, 67 (2020). https://doi.org/10.1007/s12038-020-00039-0

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