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Effects of Shading on the Synthesis of Volatile Organic Compounds in ‘Marselan’ Grape Berries (Vitis vinifera L.)

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Abstract

Light is a vital environmental factor that can affect the synthesis of volatile organic compounds (VOCs) in grape berries. However, the mechanism through which light affects the synthesis of VOCs is still unclear for wine grapes. In our study, fruit bags with light transmittances of 50%, 15%, 5%, and 0% were used to bag the ‘Marselan’ clusters 45 days after flowering, and these treatments were named A, B, C, and D, respectively. The non-bagged clusters were used as controls (CK). The types and contents of VOCs were determined using a gas chromatography–mass spectrometer (GC–MS) with the berries 35 days before harvest (S1), 25 days before harvest (S2), and at the harvest stage (S3). RNA-seq analysis was performed on S2 and S3 samples. The results showed that the types and total contents of VOCs synthesized by fatty acid metabolic and isoprene metabolic pathways decreased with a decrease in light intensity in berries at S3. The types of VOCs synthesized by the amino acid metabolic pathway were reduced under complete shading conditions, which was consistent with results of other treatments and in the CK. The total content of VOCs was significantly reduced in shaded berries. The components of VOCs were also significantly decreased in shaded berries, except for 2-heptanol, cis-7-decenal, and trans-2-hexenal. Furthermore, RNA-seq analysis revealed that the pathway related to VOC synthesis was the alpha-linolenic acid metabolic pathway, which contained 10 differentially expressed genes from the LOXO, HPL, and ADH gene families. Correlation analysis between metabolites and differentially expressed genes of this pathway showed that the synthesis of most VOCs was highly correlated with the expression levels of LOXOs (VIT_09s0002g01080, VIT_06s0004g01450), HPL (VIT_12s0059g01060), and ADHs (VIT_04s0044g01110, VIT_18s0001g15450, VIT_18s0001g15410) (r > 0.95, p < 0.5) in the fatty acid metabolic pathway. The content of 2-heptanol, cis-7-decenal, and trans-2-hexenal was highly positively correlated with the expression levels of LOXO (VIT_13s0064g01480) and ADH (VIT_18s0001g15410) (r > 0.95, p < 0.5). In conclusion, the types and contents of VOCs synthesized by fatty acid metabolic, amino acid metabolic, and isoprene metabolic pathways are reduced in harvested clusters that had been shaded, except for 2-heptanol, cis-7-decenal, and trans-2-hexenal. In addition, the development of VOCs was mainly related to the expressions of LOXO, HPL, and ADH in the fatty acid metabolic pathway.

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References

  • Alem H, Rigou P, Schneider R, Ojeda H, Torregrosa L (2019) Impact of agronomic practices on grape aroma composition: a review. J Sci Food Agric 99:975–985

    CAS  PubMed  Google Scholar 

  • Alessandrini M, Battista F, Panighel A, Flamini R, Tomasi D (2018) Effect of pre-bloom leaf removal on grape aroma composition and wine sensory profile of Semillon cultivar. J Sci Food Agric 98:1674–1684

    CAS  PubMed  Google Scholar 

  • Barros EP, Moreira N, Pereir GE, Leite SGF, Rezende CM, de Pinho PG (2012) Development and validation of automatic HS-SPME with a gas chromatography-ion trap/mass spectrometry method for analysis of volatiles in wines. Talanta 101:177–186

    Google Scholar 

  • Belancic A, Agosin E, Ibacache A, Bordeu E, Baumes R, Razungles A, Bayonove C (1997) Influence of sun exposure on the aromatic composition of Chilean Muscat grape cultivars Moscatel de Alejandria and Moscatel rosada. Am J Enol Viticul 48:181–186

    CAS  Google Scholar 

  • Belda I, Ruiz J, Esteban-Fernández A, Navascués E, Marquina D, Santos A, Moreno-Arribas MV (2017) Microbial contribution to wine aroma and its intended use for wine quality improvement. Molecules 22:189

    PubMed Central  Google Scholar 

  • Black CA, Parker M, Siebert TE, Capone DL, Francis IL (2015) Terpenoids and their role in wine flavour: recent advances. Aust J Grape Wine Res 21:582–600

    CAS  Google Scholar 

  • Boussaa F, Zaouay F, Hernandez F, Noguera-Artiaga L, Carbonell-Barrachina Ά, Melgarejo P, Mars M (2018) Cropping system contributes largely to fruit composition and sensory properties of pomegranate (Punica granatum L. var. Gabsi). S Afr J Bot 115:170–178

    CAS  Google Scholar 

  • Bureau SM, Razungles AJ, Baumes RL (2000) The aroma of Muscat of Frontignan grapes: effect of the light environment of vine or bunch on volatiles and glycoconjugates. J Sci Food Agric 80:2012–2020

    CAS  Google Scholar 

  • Cai H, An X, Han S, Jiang L, Yu M, Ma R, Yu Z (2018) Effect of 1-MCP on the production of volatiles and biosynthesis-related gene expression in peach fruit during cold storage. Postharvest Biol Technol 141:50–57

    CAS  Google Scholar 

  • Chonhenchob V, Kamhangwong D, Kruenate J, Khongrat K, Tangchantra N, Wichai U, Singh SP (2011) Preharvest bagging with wavelength-selective materials enhances development and quality of mango (Mangifera indica L.) cv. Nam Dok Mai# 4. J Sci Food Agric 91:664–671

    CAS  PubMed  Google Scholar 

  • D’Onofrio C, Matarese F, Cuzzola A (2018) Effect of methyl jasmonate on the aroma of Sangiovese grapes and wines. Food Chem 242:352–361

    PubMed  Google Scholar 

  • Dudareva N, Klempien A, Muhlemann JK, Kaplan I (2013) Biosynthesis, function and metabolic engineering of plant volatile organic compounds. New Phytol 198:16–32

    CAS  PubMed  Google Scholar 

  • Dunlevy JD, Kalua CM, Keyzers RA, Boss PK (2009) The production of flavour & aroma compounds in grape berries. In: Grapevine molecular physiology & biotechnology. Springer, Dordrecht, pp 293–340

  • El Hadi MA, Zhang FJ, Wu FF, Zhou CH, Tao J (2013) Advances in fruit aroma volatile research. Molecules 18:8200–8229

    PubMed  PubMed Central  Google Scholar 

  • Feng H, Yuan F, Skinkis P, Qian MC (2012) Effect of cluster zone leaf removal on grape sugar, acids, carotenoids, and volatile composition. Am J Enol Vitic 63:458A–458A

    Google Scholar 

  • Feng H, Yuan F, Skinkis PA, Qian MC (2015) Influence of cluster zone leaf removal on Pinot noir grape chemical and volatile composition. Food Chem 173:414–423

    CAS  PubMed  Google Scholar 

  • Ferreira V, López R, Cacho JF (2000) Quantitative determination of the odorants of young red wines from different grape varieties. J Sci Food Agric 80:1659–1667

    CAS  Google Scholar 

  • Friedel M, Frotscher J, Nitsch M, Hofmann M, Bogs J, Stoll M, Dietrich H (2016) Light promotes expression of monoterpene and flavonol metabolic genes and enhances flavour of wine grape berries (Vitis vinifera L. cv. Riesling). Aust J Grape Wine R 22:409–421

    CAS  Google Scholar 

  • Garde-Cerdán T, Gutiérrez-Gamboa G, Baroja E, Rubio-Bretón P, Pérez-Álvarez EP (2018) Influence of methyl jasmonate foliar application to vineyard on grape volatile composition over three consecutive vintages. Food Res Int 112:274–283

    PubMed  Google Scholar 

  • Granell A, Rambla JL (2013) Biosynthesis of volatile compounds. In: The molecular biology and biochemistry of fruit ripening. Wiley, New York

  • Herbert RB (1989) The biosynthesis of secondary metabolites. Springer, Dordrecht

    Google Scholar 

  • Ju YL, Liu M, Zhao H, Meng JF, Fang YL (2016) Effect of exogenous abscisic acid and methyl jasmonate on anthocyanin composition, fatty acids, and volatile compounds of Cabernet sauvignon (Vitis vinifera L.) grape berries. Molecules 21:1354

    PubMed Central  Google Scholar 

  • Kanehisa M, Goto S, Kawashima S, Okuno Y, Hattori M (2004) The KEGG resource for deciphering the genome. Nucleic Acids Res 2(Suppl_1):D277–D280

    Google Scholar 

  • Kwasniewski MT, Vanden-heuvel JE, Pan BS, Sacks GL (2010) Timing of cluster light environment manipulation during grape development affects C13 norisoprenoid and carotenoid concentrations in Riesling. J Agric Food Chem 58:6841–6849

    CAS  PubMed  Google Scholar 

  • Lashbrooke JG, Young PR, Dockrall SJ, Vasanth K, Vivier MA (2013) Functional characterisation of three members of the Vitis vinifera L. carotenoid cleavage dioxygenase gene family. BMC Plant Biol 13:156

    PubMed  PubMed Central  Google Scholar 

  • Liu C, Liu Y (2012) Impacts of shading in field on micro-environmental factors around plants and quality of pineapple fruits. J Food Agric Environ 10:741–745

    CAS  Google Scholar 

  • Livak KJ, Schmittgen TD (2011) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408

    Google Scholar 

  • Luan F, Wüst M (2002) Differential incorporation of 1-deoxy-Dxylulose into (3S)-linalool and geraniol in grape berry exocarp and mesocarp. Phytochemistry 60:451–459

    CAS  PubMed  Google Scholar 

  • Ma Z, Li W, Mao J, Li W, Zuo C, Zhao X, Dawuda MM, Shi X, Chen B (2019) Synthesis of light-inducible and light-independent anthocyanins regulated by specific genes in grape ‘Marselan’ (V. vinifera L.). Peer J 7:e6521

    PubMed  Google Scholar 

  • Mageroy MH, Tieman DM, Floystad A, Taylor MG, Klee HJ (2012) A Solanum lycopersicum catechol-O-methyltransferase involved in synthesis of the flavor molecule guaiacol. Plant J 69:1043–1051

    CAS  PubMed  Google Scholar 

  • Marais J, Hunter JJ, Haasbroek PD (1999) Effect of canopy microclimate, season and region on Sauvignon blanc grape composition and wine quality. S Afr J Enol Vitic 20:19–30

    CAS  Google Scholar 

  • Mathieu S (2005) Étude d'une carotène-dioxygenase de raisin (VvCCD1): aspects biochimiques et moléculaires. Doctoral Dissertation, Montpellier 2

  • Meyers JM, Sacks GL, Vanden Heuvel JE (2013) Glycosylated aroma compound responses in ‘Riesling’ wine grapes to cluster exposure and vine yield. Horttechnology 23:581–588

    Google Scholar 

  • Miszczak A, Forney CF, Prange RK (2005) Development of aroma volatiles and color during postharvest ripening of ‘Kent’ strawberries. J Am Soc Hortic Sci 120:650–655

    Google Scholar 

  • Morena Luna LH, Reynolds AG, di Profio FA, Zhang L, Kotsaki E (2018) Crop level and harvest date impact on four Ontario wine grape cultivars. II. Wine aroma compounds and sensory analysis. S Afr J Enol Vitic 39:246–270

    Google Scholar 

  • Rambla JL, Trapero-Mozos A, Diretto G, Rubio-Moraga A, Granell A, Gómez-Gómez L, Ahrazem O (2016) Gene-metabolite networks of volatile metabolism in Airen and tempranillo grape cultivars revealed a distinct mechanism of aroma bouquet production. Front Plant Sci 7:1619

    PubMed  PubMed Central  Google Scholar 

  • Scafidi P, Pisciotta A, Patti D, Tamborra P, DiLorenzo R, Barbagallo MG (2013) Effect of artificial shading on the tannin accumulation and aromatic composition of the Grillo cultivar (Vitis vinifera L.). BMC Plant Biol 13:175

    PubMed  PubMed Central  Google Scholar 

  • Schaffer RJ, Friel EN, Souleyre EJ, Bolitho K, Thodey K, Ledger S, Bowen JH, Ma JH, Nain B, Cohen D, Gleave AP, Crowhurst RN, Janssen BJ, Yao JL, Newcomb RD (2007) A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway. Plant Physiol 144:1899–1912

    CAS  PubMed  PubMed Central  Google Scholar 

  • Schultz H (2000) Climate change and viticulture: a European perspective on climatology, carbon dioxide and UV-B effects. Aust J Grape Wine Res 6:2–12

    CAS  Google Scholar 

  • Stevens MA (1970) Relationship between polyene-carotene content and volatile compound composition of tomatoes. Am Soc Hortic Sci Proc 95:461

    CAS  Google Scholar 

  • Sun P, Schuurink RC, Caissard JC, Hugueney P, Baudino S (2016) My way: noncanonical biosynthesis pathways for plant volatiles. Trends Plant Sci 21:884–894

    CAS  PubMed  Google Scholar 

  • Tzin V, Rogachev I, Meir S, Moyal Ben Zvi M, Masci T, Vainstein A, Aharoni A, Galili G (2013) Tomato fruits expressing a bacterial feedback-insensitive 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase of the shikimate pathway possess enhanced levels of multiple specialized metabolites and upgraded aroma. J Exp Bot 64:4441–4452

    CAS  PubMed  PubMed Central  Google Scholar 

  • Watson R, Wright CJ, McBurney T, Taylor AJ, Linforth RST (2002) Influence of harvest date and light integral on the development of strawberry flavour compounds. J Exp Bot 53:2121–2129

    CAS  PubMed  Google Scholar 

  • Williams PJ, Sefton MA, Francis IL (1992) Glycosidic precursors of varietal grape and wine flavor. In: ACS symposium series. American Chemical Society, Washington, DC, USA

  • Xie C, Mao X, Huang J, Ding Y, Wu J, Dong S, Kong L, Gao G, Li CY, Wei L (2011) KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases. Nucleic Acids Res 39(suppl 2):316–322

    Google Scholar 

  • Yang C, Wang Y, Wu B, Fang J, Li S (2011) Volatile compounds evolution of three table grapes with different flavour during and after maturation. Food Chem 128:823–830

    CAS  Google Scholar 

  • Yang M, Zhu L, Pan C, Xu L, Liu Y, Ke W, Yang P (2015) Transcriptomic analysis of the regulation of rhizome formation in temperate and tropical lotus (Nelumbo nucifera). Sci Rep 5:13059

    CAS  PubMed  PubMed Central  Google Scholar 

  • Yang Y, Jin GJ, Wang XJ, Kong CL, Liu J, Tao YS (2019) Chemical profiles and aroma contribution of terpene compounds in Meili (Vitis vinifera L.) grape and wine. Food Chem 284:155–161

    CAS  PubMed  Google Scholar 

  • Zhang B, Yin XR, Li X, Yang S, Ferguson IB, Chen K (2009) Lipoxygenase gene expression in ripening kiwifruit in relation to ethylene and aroma production. J Agric Food Chem 57:2875–2881

    CAS  PubMed  Google Scholar 

  • Zhang H, Fan P, Liu C, Wu B, Li S, Liang Z (2014) Sunlight exclusion from Muscat grape alters volatile profiles during berry development. Food Chem 164:242–250

    CAS  PubMed  Google Scholar 

  • Zhang L, Wang JW, Li GD, Zhou X, Fu WW, Jiang YG, Liu T, Ji SJ (2018) Exogenous ATP alleviated aroma fading by regulating lox, pathway and fatty acids synthesis in ‘nanguo’ pears after refrigeration. Sci Hortic 240:522–529

    CAS  Google Scholar 

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Acknowledgements

This research was funded by Discipline Construction Funds for Horticulture, Gansu Agricultural University, China (GSAU-XKJS-2018-226), Science and Technology Major Project of Gansu Province, China (18ZD2NA006).

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  1. College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, China

    Zonghuan Ma, Shijin Yang, Juan Mao, Wenfang Li, Wei Li, Cunwu Zuo, Mingyu Chu, Xin Zhao, Qi Zhou & Baihong Chen

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  1. Zonghuan Ma
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Ma, Z., Yang, S., Mao, J. et al. Effects of Shading on the Synthesis of Volatile Organic Compounds in ‘Marselan’ Grape Berries (Vitis vinifera L.). J Plant Growth Regul 40, 679–693 (2021). https://doi.org/10.1007/s00344-020-10123-2

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