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Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1

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Abstract

To valorize starchy waste from rice noodle factory, bioconversion of gelatinized starchy waste (GSW) to value-added product as L(+)-lactic acid, the monomer for polylactate synthesis, was investigated using amylolytic lactic acid bacterium, Enterococcus faecium K-1. Screening for appropriate nitrogen source to replace expensive organic nitrogen sources revealed that corn steep liquor (CSL) was the most suitable regarding high efficacy for L(+)-LA achievement and low-cost property. The successful applying statistic experimental design, Plackett-Burman design incorporated with central composite design (CCD), predicted the maximum L(+)-LA of 93.07 g/L from the optimized medium (OM) containing 125.7 g/L GSW and 207.3 g/L CSL supplemented with CH3COONa, MgSO4, MnSO4, K2HPO4, CaCl2, (NH4)2HC6H5O7, and Tween80. Minimizing the medium cost by removal of all inorganic salts and Tween80 from OM was not an effect on L(+)-LA yield. Fermentation using the optimized medium without minerals (OM-Mi) containing only GSW (125.7 g/L) and CSL (207.3 g/L) in a 10-L fermenter was also successful. Thinning GSW with α-amylase from Lactobacillus plantarum S21 increased L(+)-LA productivity in the early stage of 24-h fermentation. Not only showing the feasible bioconversion process for GSW utilizing as a substrate for L(+)-LA production, this research also demonstrated the efficient model for industrial starchy waste valorization.

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Acknowledgments

We acknowledge the Material Science Research Center, Chiang Mai University for supports and encouragement on this research.

Funding

Chiang Mai University provided financial support via the postdoctoral fellowship and adjunct professorship.

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Authors and Affiliations

  1. Division of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, Thailand

    Kridsada Unban & Chartchai Khanongnuch

  2. Faculty of Engineering and Natural Sciences, Tampere University, P.O. Box 541, FI-33014, Tampere, Finland

    Ramita Khanongnuch

  3. Division of Biochemistry and Biochemical Technology, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand

    Apinun Kanpiengjai

  4. Global Institute of Food Security and International Agriculture (GIFSIA), Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    Kalidas Shetty

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  1. Kridsada Unban
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Correspondence to Chartchai Khanongnuch.

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Unban, K., Khanongnuch, R., Kanpiengjai, A. et al. Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1. Appl Biochem Biotechnol 192, 353–366 (2020). https://doi.org/10.1007/s12010-020-03314-w

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