Abstract
The turbot Scophthalmus maximus has evolved extensive physiological ability to adapt to multiple environmental salinities. The morphological changes of the kidney indicated the adaptability difference and similarity of turbot to salinity stress. Identify transcriptome-wide differences between low-salinity seawater (LSW, salinity 5)- and high-salinity seawater (HSW, salinity 50)-acclimated kidneys of turbot to decipher the osmotic regulation mechanism. We identified 688 differentially expressed genes (DEGs) in the LSW-acclimated kidneys and 2441 DEGs in the HSW-acclimated kidneys of turbot compared with seawater-acclimated kidneys, respectively. We investigated three patterns of gene regulation to salinity stress that involved in ion channels and transporters, functions of calcium regulation, organic osmolytes, energy demand, cell cycle regulation, and cell protection. Additionally, protein-protein interaction (PPI) analysis of DEGs suggested the presence of a frequent functional interaction pattern and that crucial genes in the PPI network are involved in hyper-osmotic regulation. Based on the analysis of comparative transcriptome data and related literature reports, we conclude that the mechanisms responsible for osmotic regulation and its divergence in turbot are related to various genes that are involved in canonical physiological functions. These findings provide insight into the divergence in osmoregulation of turbot and valuable information about osmoregulation mechanisms that will benefit other studies in this field.
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Data availability
The raw reads for the next generation sequencing data have been submitted to NCBI Sequence Read Archive (SRA) with the accession number SRP153594.
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Funding
The current work was granted by the Earmarked Fund for Modern Agro-Industry Technology Research System under contract No. CARS-47-G01, and the AoShan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology under contract No. 2017ASTCP-OS04, the National Natural Science Foundation of China 41706168, the Agricultural Fine Breed Project of Shandong under contract No. 2019LZGC013, the Key Research and Development Plan of Nation under contract No. 2018YFD0900301-12, Central Public-interest Scientific Institution Basal Research Fund, YSFRI,CAFS No.20603022019019, and Yantai Science and Technology project No. 2018ZDCX021.
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Cui, W., Ma, A., Huang, Z. et al. Comparative transcriptomic analysis reveals mechanisms of divergence in osmotic regulation of the turbot Scophthalmus maximus. Fish Physiol Biochem 46, 1519–1536 (2020). https://doi.org/10.1007/s10695-020-00808-6
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DOI: https://doi.org/10.1007/s10695-020-00808-6