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The Arabidopsis AtAAE13.1 Gene Enhances Salt Stress Tolerance in Angiosperms and Gymnosperm Plant Cells

  • Cell Biology
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Abstract

The Arabidopsis malonyl-CoA synthetase gene AAE13.1 (AtAAE13.1) plays important roles in cell metabolism, plant growth and development, and environmental stress responses. However, function of AtAAE13.1 in NaCl stress tolerance is not determined yet. Cell suspension cultures of different plant species including rice (Oryza sativa L.), cotton (Gossypium hirsutum L.), and white pine (Pinus strobus L.) were transformed using Agrobacterium tumefaciens strain GV3101 harboring pBI-AtAAE13.1. After confirmation of integration of the AtAAE13.1 gene into the genome by polymerase chain reaction (PCR), Southern and northern blot analyses, NaCl stress tolerance was examined in AtAAE13.1 transgenic cells of O. sativa, G. hirsutum, and P. strobus. AtAAE13.1 expression enhanced NaCl stress tolerance by increasing cell viability and growth rate, decreasing lipid peroxidation, increasing the oxidation rate, and elevating the content of amino acids, glycolate, and phosphoglycolate, as well as elevating the amount of sucrose, glucose, and fructose. In rice cells, AtAAE13.1 elevated expression of the Ca2+-dependent protein kinase (CPK) genes under NaCl stress. Overexpression of the AtAAE13.1 gene may be useful for engineering NaCl stress tolerance in different plant species.

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Acknowledgments

We thank Dr. Prasad, Dr. Lischewski, and Dr. Page for their critical reading and suggestions during the preparation of this manuscript.

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  1. College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, Hubei, China

    Mingqin Zhou & Wei Tang

  2. CIMAS, 101 Science Drive, Duke University, Durham, NC, 27708, USA

    Wells A. Thompson & Wei Tang

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  1. Mingqin Zhou
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Correspondence to Wei Tang.

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Zhou, M., Thompson, W.A. & Tang, W. The Arabidopsis AtAAE13.1 Gene Enhances Salt Stress Tolerance in Angiosperms and Gymnosperm Plant Cells. In Vitro Cell.Dev.Biol.-Plant 56, 750–764 (2020). https://doi.org/10.1007/s11627-020-10083-y

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