Abstract
Elicitation is one of the effective methods for enhancement of metabolite production in plants. In this study we evaluated for the first time how elicitors affect biomass and phenolic production and antioxidant potential in relation to type (salicylic acid and yeast extract) and concentration of the elicitor and harvesting time in Knautia sarajevensis. Biomass production was estimated according to the control plants cultivated in liquid MS medium without eliciting agents. Total phenol, flavonoid and flavanol contents were estimated along with antioxidant activities at different harvesting times (0, 3, 7 and 15 d post-elicitation). The differences of phenolic metabolites were analysed by HPLC-UV for all treatments at all harvesting times. The best eliciting effect was recorded after 7 d by using 100 mM salicylic acid. Secondary metabolite profile analysis showed a significant increase in the content of rosmarinic acid, as well as chlorogenic and salicylic acids. The DPPH radical scavenging activity was decreased after elicitation. Increase in peroxidase activity was recorded especially after elicitation with 100 mM salicylic acid 3 d post-elicitation.
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Abraham F, Bhatt A, Keng CL, Indrayanto G, Sulaiman SF (2011) Effect of yeast extract and chitosan on shoot proliferation, morphology and antioxidant activity of Curcuma mangga in vitro plantlets. Afr J Biotechnol 10:7787–7795
Allen DE, Hatfield G (2004) Medicinal plants in folk tradition. Timber Press, Portland, p 276
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Chan Z, Tian S (2006) Induction of H2O2-metabolizing enzymes and total protein synthesis by antagonistic yeast and salicylic acid in harvested sweet cherry fruit. Postharvest Biol Technol 39:314–320
Cheng Q, He Y, Li G, Liu Y, Gao W, Huang L (2013) Effects of combined elicitors on tanshinone metabolic profiling and SmCPS expression in Salvia miltiorrhiza hairy root cultures. Molecules 18:7473–7485
Coste A, Vlase L, Halmagyi A, Deliu C, Coldea G (2011) Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum. Plant Cell Tissue Organ Cult 106:279–288
Dixon RA, Achnine L, Kota P, Liu CJ, Reddy MS, Wang L (2002) The phenylpropanoid pathway and plant defence—a genomics perspective. Mol Plant Pathol 3:371–390
Gadzovska S, Maury S, Delaunay A, Spasenoski M, Hagège D, Courtois D, Joseph C (2013) The influence of salicylic acid elicitation of shoots, callus, and cell suspension cultures on production of naphtodianthrones and phenylpropanoids in Hypericum perforatum L. Plant Cell Tissue Organ Cult 113:25–39
Gadzovska S, Maury S, Delaunay A, Spasenoski M, Joseph C, Hagege D (2007) Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant Cell Tissue Organ Cult 89:1–13
González LF, Rojas MC, Perez FJ (1999) Diferulate and lignin formation is related tobiochemical differences of wall-bound peroxidases. Phytochemistry 50:711–717
Gorni PH, Pacheco AC, Silva JFA, Moreli RR, Spera KD, Silva RMG (2019) Plant elicitation with salicylic acid increases bioactive compounds content and antioxidant activity in the infusion of Achillea millefolium L. Biosci J 35:289–295
Karalija E, Parić A (2018) Effects of salicylic acid foliar application on growth and antioxidant potential of basil. Biologica Nyssana 8:145–150
Karalija E, Zeljković SĆ, Tarkowski P, Muratović E, Parić A (2017) The effect of cytokinins on growth, phenolics, antioxidant and antimicrobial potential in liquid agitated shoot cultures of Knautia sarajevensis. Plant Cell Tissue Organ Cult 131:347–357
Loc NH, Anh NHT, Khuyen LTM, An TNT (2014) Effects of yeast extract and methyl jasmonate on the enhancement of solasodine biosynthesis in cell cultures of Solanum hainanense Hance. J Biol Sci Biotechnol 3:1–6
Mahalakshmi R, Eganathan P, Parida AK (2013) Salicylic acid elicitation on production of secondary metabolite by cell cultures of Jatropha curcas L. Int J Pharm Pharm Sci 5:655–659
Maqsood M, Abdul M (2017) Yeast extract elicitation increases vinblastine and vincristine yield in protoplast derived tissues and plantlets in Catharanthus roseus. Rev Bras 27:549–556
Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG (2005) Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem 91:571–577
Mendoza D, Cuaspud O, Arias JP, Ruiz O, Arias M (2018) Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana. Biotechnol Rep 19:e00273. https://doi.org/10.1016/j.btre.2018.e00273
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
Ordonez AAL, Gomez JD, Vattuone MA (2006) Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chem 97:452–458
Owis AI, Abdelwahab NS, Abul-Soad AA (2016) Elicitation of phenolics from the micropropagated endangered medicinal plant Calligonum polygonoides L.(Polygonoaceae). Pharmacogn Mag 12:465–470
Parić A, Karalija E (2018) The effect of salicylic acid on response to water deficit in basil. Genet Appl 2(1):42–48
Pauw BB, Duijn JW, Memelink KJ (2004) Activation of the oxidative burst by yeast elicitor in Catharanthus roseus cells occurs independently of the activation of genes involved in alkaloid biosynthesis. Plant Mol Biol 55:797–805
Pérez MGF, Guzmán NER, Silva EMS, Piña GL, Camacho RR (2014) Effect of chemical elicitors on peppermint (Mentha piperita) plants and their impact on the metabolite profile and antioxidant capacity of resulting infusions. Food Chem 156:273–278
Pirian K, Piri K (2013) Influence of yeast extract as a biotic elicitor on noradrenaline production in hairy root culture of Portulaca oleracea L. Int J Agron Plant Prod 4:2960–2964
Pitta-Alvarez SI, Spollansky TC, Giulietti AM (2000) The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzym Microb Technol 26:252–258
Sharma U, Agrawal V (2018) In vitro shoot regeneration and enhanced synthesis of plumbagin in root callus of Plumbago zeylanica L.-an important medicinal herb. In Vitr Cell Dev Biol-Plant 54:423–435
Shinde AM, Malpathak N, Fulzele DP (2009) Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. using elicitation and precursor feeding. Biotechnol Bioprocess Eng 14:288–294
Šabanović M, Parić A, Briga M, Karalija E (2018) Effect of salicylic acid seed priming on resistance to high levels of cadmium in lettuce (Lactuca sativa L.). G&A 2:67–72
Udomsuk L, Jarukamjorn K, Tanaka H, Putalun W (2011) Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation. Biotechnol Lett 33:369–374
Wang M, Lamers RJA, Korthout HA, van Nesselrooij JH, Witkamp RF, van der Heijden R, Voshol PJ, Havekes LM, Verpoorte R, van der Greef J (2005) Metabolomics in the context of systems biology: bridging traditional Chinese medicine and molecular pharmacology. Phytother Res 19:173–182
Wolfe K, Wu X, Liu RH (2003) Antioxidant activity of apple peels. J Agric Food Chem 51:609–614
Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333
Złotek U, Świeca M (2016) Elicitation effect of Saccharomyces cerevisiae yeast extract on main health-promoting compounds and antioxidant and anti-inflammatory potential of butter lettuce (Lactuca sativa L.). J Sci Food Agric 96:2565–2572
Acknowledgements
This work was supported by project No. RO0418 (sustainable systems and technologies, improving crop production for higher quality of production of food, feed, and raw materials, under conditions of changing climate) funded by the Ministry of Agriculture, Czech Republic.
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Editor: Yong Eui Choi
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Karalija, E., Zeljković, S.Ć. & Parić, A. Harvest time–related changes in biomass, phenolics and antioxidant potential in Knautia sarajevensis shoot cultures after elicitation with salicylic acid and yeast. In Vitro Cell.Dev.Biol.-Plant 56, 177–183 (2020). https://doi.org/10.1007/s11627-019-10028-0
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DOI: https://doi.org/10.1007/s11627-019-10028-0