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The isotopic nitrogen turnover rate as a proxy to evaluate in the long-term the protein turnover in growing ruminants

Published online by Cambridge University Press:  20 March 2020

Gonzalo Cantalapiedra-Hijar Open the ORCID record for Gonzalo Cantalapiedra-Hijar [Opens in a new window] ,
Hélène Fouillet Open the ORCID record for Hélène Fouillet [Opens in a new window] ,
Céline Chantelauze ,
Nadezda Khodorova ,
Lahlou Bahloul  and
Isabelle Ortigues-Marty Open the ORCID record for Isabelle Ortigues-Marty [Opens in a new window]
Gonzalo Cantalapiedra-Hijar*
Affiliation:
INRAE, Université Clermont Auvergne, Vetagro Sup, UMRH, F-63122, Saint-Genès-Champanelle, France
Hélène Fouillet
Affiliation:
Université Paris-Saclay, AgroParisTech, INRAE, UMR PNCA, F-75005, Paris, France
Céline Chantelauze
Affiliation:
INRAE, Université Clermont Auvergne, Vetagro Sup, UMRH, F-63122, Saint-Genès-Champanelle, France
Nadezda Khodorova
Affiliation:
Université Paris-Saclay, AgroParisTech, INRAE, UMR PNCA, F-75005, Paris, France
Lahlou Bahloul
Affiliation:
Adisseo France S.A.S., Antony, France
Isabelle Ortigues-Marty
Affiliation:
INRAE, Université Clermont Auvergne, Vetagro Sup, UMRH, F-63122, Saint-Genès-Champanelle, France
*
Author for correspondence: Gonzalo Cantalapiedra-Hijar, E-mail: gonzalo.cantalapiedra@inrae.fr
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Abstract

Protein turnover is an energy-consuming process that is essential for ensuring the maintenance of living organisms. Gold standard methods for whole-body protein turnover (WBPT) measurement have inherent drawbacks precluding their generalization for large farm animals and use during long periods. Here, we proposed a non-invasive proxy for the WBPT over a long period of time and in a large number of beef cattle. The proxy is based on the rate at which urine-N and plasma proteins are progressively depleted in terms of 15N after a slight decrease in the isotopic N composition of the diet (i.e. diet switch). We aimed to test the ability of this proxy to adequately discriminate the WBPT of 36 growing-fattening young bulls assigned to different dietary treatments known to impact the WBPT rate, with different protein contents (normal v. high) and amino acid profiles (balanced v. unbalanced in methionine). The 15N depletion rate found in plasma proteins represented their fractional synthesis rate, whereas the slow depletion rate found in urine was interpreted as a proxy of the WBPT. The proxy tested in urine suggested different WBPT values between the normal- and high-protein diets but not between the balanced and unbalanced methionine diets. In contrast, the proxy tested in plasma indicated that both dietary conditions affected the fractional synthesis rate of plasma proteins. We considered that the rate at which urine is progressively 15N-depleted following an isotopic diet switch could be proposed as a non-invasive proxy of the WBPT rate in large farm animals.

Keywords

15Nbeef cattleisotopeprotein turnoverruminant
Type
Modelling Animal Systems Research Paper
Information
The Journal of Agricultural Science , Volume 157 , Issue 9-10 , December 2019 , pp. 701 - 710
Copyright
Copyright © Cambridge University Press 2020

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