Abstract
Citrus tatter leaf virus (CTLV), a strain of apple stem grooving virus, is a virus of citrus that is of commercial importance for trifoliate orange (Poncirus trifoliata) and trifoliate hybrid rootstocks. Bud-union crease is a CTLV-induced incompatibly symptom observed with these rootstocks and citrus scions which results in stunted tree growth. CTLV is a regulated pathogen for most citrus budwood certification programs worldwide. Due to the requisite virus testing, commercially supplied scion budwood would therefore seldom be a point of contamination. However, citrus rootstocks are primarily propagated by seed and the potential for seed transmission of CTLV in citrus is unclear and remains a potential source of infection. A single, unconfirmed report of seed transmission of CTLV in ‘Eureka’ lemon (Citrus × limon) exists, and although this report was not of a rootstock cultivar, it does point to the risk of seed transmission in citrus and close relatives in the family Rutaceae. CTLV-positive ‘Meyer’ lemon (C. × limon) trees in an experimental plot in the Eastern Cape Province of South Africa presented an opportunity to again test seed transmission in citrus. Seed was obtained from the infected ‘Meyer’ lemon trees, and 1164 seedlings were grown in an insect-secure greenhouse and screened for CTLV in pooled batches by conventional and real-time RT-PCR. No positive samples were detected and results indicated a lack of virus seed transmission in ‘Meyer’ lemon. Findings of this study are therefore not supportive of CTLV seed transmission in citrus.
References
Calavan EC, Christiansen DW, Roistacher CN (1963) Symptoms associated with tatter leaf virus infection of Troyer citrange rootstocks. Plant Dis Rep 47:971–975
Cook G, van Vuuren SP, Breytenbach JHJ, Burger JT, Maree HJ (2016) Expanded strain-specific RT-PCR assay for differential detection of currently known Citrus tristeza virus strains: a useful screening tool. J Phytopathol 164:847–851. https://doi.org/10.1111/jph.12454
Coordinators NR (2012) Database resources of the National Center for Biotechnology Information. Nucleic Acids Res 41:D8–D20
Da Graca JV (1977) Citrus tatter leaf virus in South African Meyer lemon. Citrus Subtrop Fruit J 529:18
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Inouye N, Maeda T, Mitsuhata K (1979) Citrus tatter leaf virus isolated from lily. Jpn J Phytopathol 45:712–720. https://doi.org/10.3186/jjphytopath.45.712
Ke C, Wu RJ (1991) Occurrence and distribution of citrus tatter leaf in Fujian, China. In: International organization of citrus virologists conference proceedings (1957–2010), 11(11). https://escholarship.org/uc/item/5p56w0tj. Accessed 1 June 2019
Liu KH, Song Z, Zhou Y, Li ZA, Zhou CY (2011) Detection of citrus tatter leaf virus by real-time RT-PCR. In: International organization of citrus virologists conference proceedings (1957–2010), 18(18). https://escholarship.org/uc/item/7gq3t4dm. Accessed 1 June 2019
Marais LJ, Lee RF (1986) Citrange stunt virus associated with decline of shamouti on swingle citrumelo rootstock in South Africa. Plant Dis 70:892
Miyakawa T, Tsuji M (1988) The association of tatterleaf virus with budunion crease of trees on trifoliate orange rootstock. In: International organization of citrus virologists conference proceedings (1957–2010), 10(10). https://escholarship.org/uc/item/7gv7436m. Accessed 1 June 2019
Nishio T, Kawai A, Kato M, Kobayashi T (1982) A sap-transmissible closterovirus in citrus imported from China and Formosa. Res Bull Plant Prot Serv Jpn 18:11–18
Roistacher CN, Nauer EM, Wagner RL (1980). Transmissibility off Cachexia, Dweet Mottle, Psorosis, tatterleaf and infectious variegation viruses on knife blades and its prevention. In: International organization of citrus virologists conference proceedings (1957–2010), 8(8). https://escholarship.org/uc/item/6s84z3zj. Accessed 1 June 2019
Saunt J (2000) Citrus rootstocks. In: Citrus varieties of the world. Sinclair Int. Ltd, Norwich, England, pp 145–156
Semancik JS, Weathers LG (1965) Partial purification of a mechanically transmissible virus associated with tatter leaf of citrus. Phytopathology 55:1354–1358
Tan S-h, Osman F, Bodaghi S, Dang T, Greer G, Huang A, Hammado S, Abu-Hajar S, Campos R, Vidalakis G (2019) Full genome characterization of 12 citrus tatter leaf virus isolates for the development of a detection assay. PLoS ONE 14:e0223958. https://doi.org/10.1371/journal.pone.0223958
Tanner JD, Kunta JV, Da Graca JV, Skaria M, Nelson SD (2011) Evidence of low rate of seed transmission of Citrus tatter leaf virus in citrus. International organization of citrus virologists conference proceedings (1957–2010), 18(18). https://escholarship.org/uc/item/0b67q81h. Accessed 1 June 2019
Tatineni S, Afunian MR, Hilf ME, Gowda S, Dawson WO, Garnsey SM (2009) Molecular characterization of Citrus tatter leaf virus historically associated with Meyer lemon trees: complete genome sequence and development of biologically active in vitro transcripts. Phytopathology 99:423–431
Wallace JM, Drake RJ (1962) Tatter leaf, a previously undescribed virus effect in citrus. Plant Dis Rep 46:211–212
Wallace JM, Drake RJ (1963) New information on symptom effects and host range of the citrus tatter-leaf virus. Plant Dis Rep 47(5):352–353
Yoshikawa N (2000) Apple stem grooving virus. CMI/AAB Descriptions of Plant Viruses No. 376
Yoshikawa N, Sasaki E, Kato M, Takahashi T (1992) The nucleotide sequence of apple stem grooving capillovirus genome. Virology 191:98–105
Yoshikawa N, Sasamoto K, Sakurada M, Takahashi T, Yanase H (1996) Apple stem grooving and citrus tatter leaf capilloviruses obtained from a single shoot of Japanese pear (Pyrus serotina). Ann Phytopathol Soc Jpn 62:119–124
Acknowledgements
We thank Nikki Combrink of the Agricultural Research Council—Tropical and Subtropical Crops for harvesting and supplying the seed for this study.
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This study was funded by Citrus Research International (Project 1144).
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Cook, G., Steyn, C., Breytenbach, J.H.J. et al. No detection of seed transmission of citrus tatter leaf virus in ‘Meyer’ lemon. J Plant Dis Prot 127, 895–898 (2020). https://doi.org/10.1007/s41348-020-00329-7
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DOI: https://doi.org/10.1007/s41348-020-00329-7