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Piperacillin Encapsulation in Nanoliposomes Using Modified Freeze-Drying of a Monophase Solution Method: Preparation, Characterization and In Vitro Antibacterial Activity

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Abstract

Piperacillin (Pip) is a broad spectrum β-lactam against most Gram-positive and Gram-negative aerobic and anaerobic bacteria. However, bacterial resistance restricts its benefits for the treatment of infectious diseases. Recently, nanoliposomal systems have been investigated as encouraging strategies to address this issue owing to their immense potential. We aimed to encapsulate Pip in liposomal nanoparticles and study their antibacterial activities in vitro against Pseudomonas aeruginosa (P. aeruginosa). Different liposomes were prepared based on the freeze-drying of a monophase solution method. Then, they were characterized in terms of size, zeta potential, polydispersity-index, and morphology. For further analysis, spectra of ATR-FTIR and XRD were taken for liposomal Pip. Encapsulation efficiency (EE) was determined via agar diffusion assay. Also, minimum inhibitory concentrations (MICs) were investigated by the standard broth macro-dilution method. The liposomes were from 100.9 to 444.13 nm with z-potential of − 30.70 to − 10.57 mV. EE of the selected formulation was 53.1%. TEM results showed that the liposomes were nanosized and almost spherical. ATR-FTIR results confirmed the full encapsulation of Pip in nanoliposomes. The X-ray pattern indicated that the liposomal Pip was amorphous. The MIC (10.6 µg/ml) of the nanoliposomal Pip against P. aeruginosa was one-half of the MIC (21.25 µg/ml) of free Pip for the same organisms. Considering four aspects (nanosized liposomes, no need for sterilization, suitable EE and enhanced antibacterial effects), this preparation method seems promising and may be used to overcome the bacterial resistance relative to Pip.

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Authors and Affiliations

  1. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Pouria Savadi, Telli Taghavi-Fard & Nastaran Hashemzadeh

  2. Infectious and Tropical Diseases Research Center & Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

    Morteza Milani

  3. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Vahid Panahi

  4. School of Medical Sciences and Menzies Health Institute Queensland, Griffith University, Southport, Australia

    Nigel A. J. McMillan

  5. Faculty of Pharmacy & Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Somayeh Hallaj-Nezhadi

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  1. Pouria Savadi
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  2. Telli Taghavi-Fard
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  3. Morteza Milani
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  4. Nastaran Hashemzadeh
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Contributions

PS: Performed some of the experiments and co-wrote the paper. TT-F: Performed experiments. MM: Designed some of the experiments. NH: performed some of the experiments and co-wrote the paper. VP: performed some of the experiments. NM: Helped in the method for the preparation of nanoliposomes and paper revision. SH-N: Designed, analyzed data, and supervised the research.

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Correspondence to Somayeh Hallaj-Nezhadi.

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Savadi, P., Taghavi-Fard, T., Milani, M. et al. Piperacillin Encapsulation in Nanoliposomes Using Modified Freeze-Drying of a Monophase Solution Method: Preparation, Characterization and In Vitro Antibacterial Activity. Curr Microbiol 77, 2356–2364 (2020). https://doi.org/10.1007/s00284-020-02008-0

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