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Complete genome sequence of a new bipartite begomovirus associated with leaf curl disease of Capsicum annum

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Abstract

The complete bipartite genome, consisting of DNA-A and DNA-B, of a novel begomovirus isolate associated with apical leaf curling and crinkled leaf disease of Chili (Capsicum annum) from New Delhi, India was cloned and sequenced. The sequence of DNA-A (2737 nt) and DNA-B (2692 nt) of the virus was submitted to NCBI, USA under the accessions MK069591 & MG597211, respectively. Sequence identity of the common region (CR) and presence of identical iterons (GAGTG) between the DNA-A and DNA-B clones indicate that they constitute a related pair. The virus corresponds to a novel species of tomato leaf curl virus and sequence analysis has ruled out the involvement of recombination events in its evolution. Therefore, we report the complete nucleotide sequence of a new bipartite begomovirus infecting Capsicum annum, a vegetable crop communally cultivated throughout India.

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Acknowledgements

AKS is thankful for financial support from the DBT-RA Program in Biotechnology and Life Sciences.

Funding

This study was funded by DBT-RA 2016, Department of Biotechnology, Ministry of Science and Technology, Govt. India.

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Authors and Affiliations

  1. Plant RNAi Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India

    Anurag Kumar Sahu & Neeti Sanan-Mishra

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  1. Anurag Kumar Sahu
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  2. Neeti Sanan-Mishra
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Correspondence to Neeti Sanan-Mishra.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Supplementary file1 (DOC 60 kb)

Supplementary Fig. S1.

Rolling circle amplification (RCA) of virus infected chili samples and cloning of the amplified fragment in pTZ57R/T vector. A. Rolling circle amplification (RCA) of six individual virus infected chili samples. The amplification product of ~2.7 Kb is shown. B. The RCA products were cloned and checked by restriction digestion. The enzymes used for digestion are mentioned over each lane. lane 1: uncut (UC) plasmid; lane 2: pTZ57R/T-DNA-A digested with EcoRI. Two bands correspond to the plasmid backbone and the 2.7 Kb insert; lane 3: pTZ57R/T-DNA-A digested with HindIII. Single band correspond to the linearized plasmid backbone containing the insert; lane 4: 1 Kb marker (MW) (TIF 299 kb)

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Sahu, A.K., Sanan-Mishra, N. Complete genome sequence of a new bipartite begomovirus associated with leaf curl disease of Capsicum annum. 3 Biotech 10, 235 (2020). https://doi.org/10.1007/s13205-020-02220-6

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