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Enterocin M-Producing Enterococcus faecium CCM 8558 Demonstrating Probiotic Properties in Horses

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Abstract

The effects of non-authochtonous Enterococcus faecium AL41 = CCM 8558, enterocin M-producing and probiotic strain were tested on the microbiota, phagocytic activity, hydrolytic enzymes, biochemical parameters and dry matter in horses based on its previous benefits demonstrated in other animals. E. faecium CCM 8558 sufficiently colonized the digestive tract of horses. At day 14, its counts reached 2.35 ± 0.70 CFU/g (log 10) on average. The identity of CCM 8558 was confirmed by means of PCR after its re-isolation from horse faeces. The inhibition activity of CCM 8558 was demonstrated against Gram-negative aeromonads, counts of which were significantly reduced (P < 0.001). After 14 days application of CCM 8558, a tendency towards increased phagocytic activity (PA) was measured; PA value was 73.13% ± 8.55 on average at day 0/1; at day 14, it was 75.11 ± 8.66%. Cellulolytic, xylanolytic and pectinolytic activity in horse faeces was significantly increased (P < 0.001) at day 14 (after CCM 8558 application) and amylolytic activity as well (P < 0.01) compared to day 0/1. Inulolytic activity increased with mathematical difference 1.378. Dry matter value reached 20.81 ± 2.29% on average at day 0/1; at day 14, it was 20.77 ± 2.59% (P = 0.9725). Biochemical parameters were influenced mostly in the physiological range. These results achieved after application of CCM 8558 in horses are original, giving us further opportunity to continue these studies, to measure additional parameters and to show the benefits of CCM 8558 application in horses.

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Availability of Data and Material

The datasets used and/or analysed during the current study are available from the corresponding author upon reasonable request.

Abbreviations

CFU:

colony-forming unit

log 10:

logarithmic 10

g:

gram

%:

percentage

van gene:

vancomycin gene

CCM:

Culture Collection of Microorganisms

log cycle:

logarithmic growth phase

E:

Enterococcus

μmoL/g/DM/min:

micromol per gram of dry matter per minute

TP:

total protein

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Acknowledgements

We would like to thank Mrs. Margita Bodnárová for her excellent laboratory work. Our preliminary results were presented in poster form at the International Scientific Conference on Probiotics and Prebiotics, 16.-23.6. 2016 in Budapest, Kubašová et al., E. faecium AL41 and its Application in Horses; Proceedings of the International Scientific Conference on Probiotics and Prebiotics, 103, ISBN:978-80-89589-14-2. We would like also to thank Mr. Andrew Billingham, native-speaking person for his English language checking.

Funding

The results achieved were funded by the Scientific Grant Agency of the Ministry for Education, Research and Sport of the Slovak Republic and the Slovak Academy of Sciences (Vega) within the projects 2/0006/17 and 2/0012/16. The role of the agency is important for us to receive money for experimental work and financial support with material. Part of results was also achieved within a joint Polish-Slovak project for the years 2019–2021 between the Polish Academy of Sciences and The Slovak Academy of Sciences (Benefits of bacteriocins application in horse diet).

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Authors and Affiliations

  1. Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia

    Andrea Lauková, Ivana Kubašová, Viola Strompfová & Monika Pogány Simonová

  2. University of Veterinary Medicine and Pharmacy, Komenského 73, 048 03, Košice, Slovakia

    Eva Styková, Soňa Gancarčíková, Iveta Plachá & Igor Valocký

  3. Polish Academy of Sciences, The Kielanowski Institute of Animal Physiology and Nutrition, Instytucka 3, 05 110, Jablonna, Poland

    Renata Miltko & Grzegorz Belzecki

Authors
  1. Andrea Lauková
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  2. Eva Styková
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  3. Ivana Kubašová
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  4. Viola Strompfová
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  5. Soňa Gancarčíková
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  6. Iveta Plachá
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  7. Renata Miltko
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  8. Grzegorz Belzecki
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  9. Igor Valocký
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  10. Monika Pogány Simonová
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Contributions

AL—study concept and design, results evaluation, manuscript writing; ES—horse targeting, E. faecium strain administration in horses, blood sampling; IK—sampling treatment for microbiota and dry matter analysis; VS—sampling treatment for microbiota; SG—biochemical analyses; IP—phagocytic activity testing; RM—enzymatic activities; GB—enzymatic activities; IV—blood sampling; MPS—microbiota testing (PCR).

Corresponding author

Correspondence to Andrea Lauková.

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The authors declare that they have no competing interests.

Ethical Approval

All procedures used in these experiments were approved by the horses’owners and by the Ethics Committee of the Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences in Košice (Slovakia).

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Lauková, A., Styková, E., Kubašová, I. et al. Enterocin M-Producing Enterococcus faecium CCM 8558 Demonstrating Probiotic Properties in Horses. Probiotics & Antimicro. Prot. 12, 1555–1561 (2020). https://doi.org/10.1007/s12602-020-09655-6

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