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Automated Pressurized Liquid Extraction of Microbial Lipids from Oleaginous Yeasts

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Abstract

The lipids produced by oleaginous microbes are considered sustainable resources for biofuels. To facilitate controlled lipid production and lipid analysis, more efficient lipid extraction methods are required. This study describes the automated pressurized liquid extraction (APLE) method for lipid extraction from dried cells of the oleaginous yeast species Rhodosporidium toruloides and Cryptococcus curvatus. Cells were mixed with diatomite in a mortar, added to the sample chamber, and treated with a mixture of chloroform and methanol at 105 °C. More than 95% lipids were extracted. Analysis by using high-performance thin-layer chromatography showed that the neutral lipid contents in the obtained samples by APLE method were similar to those by the ball milling–assisted extraction method. The lipids had an essentially identical fatty acid composition compared with lipids extracted with the acid-heating extraction (AHE) method. This demonstrated that lipids can be efficiently extracted from oleaginous yeasts in less time and without harsh pretreatment procedures.

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Acknowledgments

We are indebted to Prof. Zongbao K. Zhao for expert opinion and consultation of Dr. Hongwei Shen for help on experimental design, Mr. Xiao Liu for assistance on APLE, and Mr. Xiaozan Dai for providing the raw material. We would like to express thanks to Energy Biotechnology Platform of Dalian Institute of Chemical Physics, CAS.

Funding

This work was supported by the National Natural Science Foundation of China (No. 51761145014).

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Authors and Affiliations

  1. Laboratory of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China

    Qiang Li, Rasool Kamal, Yadong Chu, Qian Wang, Xue Yu & Qitian Huang

  2. University of Chinese Academy of Sciences, 19 Yuquan Lu, Beijing, 100049, China

    Qiang Li & Rasool Kamal

  3. Dalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, 116023, China

    Yadong Chu, Qian Wang, Xue Yu & Qitian Huang

Authors
  1. Qiang Li
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  2. Rasool Kamal
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  3. Yadong Chu
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  4. Qian Wang
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  5. Xue Yu
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  6. Qitian Huang
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Correspondence to Qitian Huang.

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Highlights

1. Automated pressurized liquid extraction was used to extract lipids directly.

2. Almost 100% lipids were extracted from oleaginous yeasts within 40 min.

3. Fatty acid profiles of lipids are identical to those reported in the literature.

4. This method is valuable for microbial lipid analysis.

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Li, Q., Kamal, R., Chu, Y. et al. Automated Pressurized Liquid Extraction of Microbial Lipids from Oleaginous Yeasts. Appl Biochem Biotechnol 192, 283–295 (2020). https://doi.org/10.1007/s12010-020-03331-9

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  • DOI: https://doi.org/10.1007/s12010-020-03331-9

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