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Morphological and molecular assessment of large sea anemones (Actiniaria: Actiniidae) in Newfoundland (eastern Canada)

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Abstract

Literature records of large actiniid sea anemones along the Atlantic coast of Canada currently include three Urticina species: U. felina, U. fecunda and U. crassicornis. The findings of the present morphological and molecular study conducted in eastern Newfoundland suggest that U. felina is often misidentified, and that this region may only harbor two similar-looking species: U. crassicornis and Cribrinopsis similis. The latter were identified using genetic analysis and comparison of key characters with the same species collected from other regions of the North Atlantic (Barents Sea), whereas no specimen corresponding to U. felina was found. Mitochondrial gene sequences of U. crassicornis, U. felina and C. similis were identical except for a different haplotype found in several specimens of U. crassicornis (with one nucleotide substitution), in contrast to five nucleotide insertions in 16S rRNA fragments of U. fecunda. Phylogenetic analysis based on three mitochondrial and two nuclear gene fragments revealed that the most closely related species among the above-mentioned were U. crassicornis and U. felina, nevertheless U. fecunda groups in the same clade as the Urticina species.

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References

  • Agassiz L (1847) Lettre de M. L. Agassiz à M. de Humboldt, datée de Boston, le 30 septembre 1847. Revue Zoologique par la Société Cuvierienne, Paris, pp 394–401

  • Bocharova ES (2015) Reproductive biology and genetic diversity of the sea anemone Aulactinia stella (Verrill, 1864). Hydrobiologia 759:27–38

    Article  CAS  Google Scholar 

  • Bolton JJ (1981) Community analysis of vertical zonation patterns on a Newfoundland rocky shore. Aquat Bot 10:299–316

    Article  Google Scholar 

  • Brunel P, Bossé L, Lamarche G (1998) Catalogue of the marine invertebrates of the estuary and Gulf of Saint Lawrence. Canadian Special Publication of Fisheries and Aquatic Sciences 126. NRC Research Press, Ottawa, pp 1–405

    Google Scholar 

  • Carlgren O (1921) Actiniaria. Part 1. Dan Ingolf Exped 5:1–241

    Google Scholar 

  • Carlgren O (1928) Ceriantharier, Zoantharieroch Actiniarier. Conspec Faunae Greenl Medd Grönland 23:251–308

    Google Scholar 

  • Carlgren O (1945) Further contributions to the knowledge of the cnidom in the anthozoa especially in the Actiniaria. Lunds Univ Arsskr N.F. Adv 241:1–24

    Google Scholar 

  • Carlgren O (1949) A survey of the Ptychodactiaria, Corallimoropharia and Actiniaria. Kungliga Svenska Vetenskapsakademiens Handlingar 1:1–121

    Google Scholar 

  • Chia FS, Spaulding J (1972) Development and juvenile growth of the sea anemone, Tealia crassicornis. Biol Bull 142:206–218

    Article  CAS  PubMed  Google Scholar 

  • Collie JS, Hermsen JM, Valentine PC (2009) Recolonization of gravel habitats on Georges Bank (northwest Atlantic). Deep Sea Res Part II Top Stud Oceanogr 56(19–20):1847–1855

    Article  Google Scholar 

  • Daly M, Fautin D (2018) World list of Actiniaria. Urticina felina (Linnaeus, 1761). World Register of marine species. https://www.marinespecies.org/aphia.php?p=taxdetails&id=100834. Accessed 24 Dec 2019

  • Fautin DG (2013) Hexacorallians of the World. https://hercules.kgs.ku.edu/hexacoral/anemone2/index.cfm. Accessed 24 Dec 2019

  • Geller JB, Walton ED (2001) Breaking up and getting together: evolution of symbiosis and cloning by fission in sea anemones (Genus Anthopleura). Evolution 55:1781–1794

    Article  CAS  PubMed  Google Scholar 

  • Hand C (1955) The sea anemones of Central California. Part 2. The Endomyarian and Mesomyarian anemones. Wasmann J Biol 13:37–99

    Google Scholar 

  • den Hartog JC, Ates RML (2011) Actiniaria from Ria de Arosa, Galicia, northwestern Spain, in the Netherlands Centre for Biodiversity Naturalis, Leiden. Zool Meded 85:11–53

    Google Scholar 

  • den Hartog JC (1995) The genus Telmatactis Gravier, 1916 (Actiniaria: Isophellidae) in Greece and the eastern Mediterranean. Zool Meded 69:153–176

    Google Scholar 

  • Haydar D (2011) What is natural? The scale of cryptogenesis in the North Atlantic Ocean. Divers Distrib 18:101–110

    Article  Google Scholar 

  • Himmelman JH, Cardinal A, Bourget E (1983) Community development following removal of urchins, Strongylocentrotus droebachiensis, from the rocky subtidal zone of the St. Lawrence Estuary, Eastern Canada. Oecologia 59:27–39

    Article  CAS  PubMed  Google Scholar 

  • Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    Article  CAS  PubMed  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Larson PG, Hamel J-F, Mercier A (2012) Redescription and notes on the reproductive biology of the sea anemone Urticina fecunda (Verrill, 1899), comb. nov. (Cnidaria: Actiniaria: Actiniidae). Zootaxa 3523:69–79

    Article  Google Scholar 

  • Lavergne Y, Himmelman JH (1984) Localisation des stocks d'oursins de l'estuaire du Saint-Laurent et leur situation dans la communauté benthique. Gouvernement du Québec, Direction des pêches maritimes, Direction de la recherche scientifique et technique, No. 108

  • Logan A (1988) A sublittoral hard substrate epibenthic community below 30 m in Head Harbour Passage, New Brunswick, Canada. Estuarine Coastal Shelf Sci 27:445–459

    Article  Google Scholar 

  • Manuel RL (1988) British Anthozoa. Synopses of the British Fauna, New Series 18. Academic Press, London, pp 1–241

    Google Scholar 

  • McMurrich JP (1911) The Actiniaria of Passamaquoddy Bay, with a discussion of their synonymy. Trans R Soc Can 34:59–83

    Google Scholar 

  • Mercier A, Sun Z, Hamel J-F (2011) Internal brooding favours pre-metamorphic chimerism in a non-colonial cnidarian, the sea anemone Urticina felina. Proc R Soc Lond B Biol Sci 278:3517–3522

    Article  Google Scholar 

  • Morrison KJ, Redden AM (2012) Bottom substrate and associated epibenthic biota of the FORCE Tidal Energy Test Site in the Minas Passage, Bay of Fundy. Dissertation, Acadia University

  • Müller OF (1776) Zoologicae Danicae Prodromus, seuanimalium Daniaeet Norvegia Indigenarumcharactees, Nomina et Synonyma imprimus popularium. Hallageriis, Havinae

  • Murillo FJ, Serrano A, Kenchington E, Mora J (2016) Epibenthic assemblages of the Tail of the Grand Bank and Flemish Cap (northwest Atlantic) in relation to environmental parameters and trawling intensity. Deep Sea Res I 109:99–122

    Article  Google Scholar 

  • Nadon MO, Himmelman JH (2010) The structure of subtidal food webs in the northern Gulf of St. Lawrence, Canada, as revealed by the analysis of stable isotopes. Aquat Living Resour 23(2):167–176

    Article  Google Scholar 

  • Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New York

    Google Scholar 

  • Novaczek E, Devillers R, Edinger E, Mello L (2017) High-resolution seafloor mapping to describe coastal denning habitat of a Canadian species at risk: Atlantic wolffish (Anarhichas lupus). Can J Fish Aquat Sci 74:2073–2084

    Article  Google Scholar 

  • Sanamyan N, Sanamyan K (2006) The genera Urticina and Cribrinopsis (Anthozoa: Actiniaria) from the north-western Pacific. J Nat Hist 40:359–393

    Article  Google Scholar 

  • Sanamyan K, Sanamyan N (2012) Isopropanol—mineral oil method in histology. In: Conservation of biodiversity of Kamchatka and coastal waters: materials of XIII international scientific conference, dedicated to the 75th anniversary of S.A. Dyrenkov’s birthday. Kamchat Press, Petropavlovsk-Kamchatsky, pp 155–159

  • Sanamyan N, Sanamyan K, McDaniel N (2013) Two new shallow water sea anemones of the family Actiniidae (Cnidaria: Anthozoa: Actiniaria) from British Columbia (NE Pacific). Invertebr Zool 9:199–216

    Article  Google Scholar 

  • Sanamyan N, Sanamyan K, McDaniel N, Bocharova ES (2018) First record of two genera of sea anemones (Cnidaria: Actiniaria), Octineon and Edwardsiella, from the North Pacific Ocean. Invertebr Zool 15:1–19

    Article  Google Scholar 

  • Sanamyan NP, Sanamyan KE, McDaniel N, Martynov AV, Korshunova TA, Bocharova ES (2019) Sea anemones of the genus Cribrinopsis (Actiniaria: Actiniidae) from British Columbia with revision of the type specimens and description of a new species. Mar Biodivers 49:1951–1969

    Article  Google Scholar 

  • Sanamyan N, Sanamyan K, Tabachnick K (2012) The first species of Actiniaria, Spongiactis japonica gen. n., sp. n. (Cnidaria: Anthozoa) an obligate symbiont of a glass sponge. Invertebr Zool 9:127–141

    Article  Google Scholar 

  • Saucier EH, Sajjadi A, France S (2017) A taxonomic review of the genus Acanella (Cnidaria: Octocorallia: Isididae) in the North Atlantic Ocean, with descriptions of two new species. Zootaxa 4323:359–390

    Article  Google Scholar 

  • Schmidt H (1969) Die Nesselkapseln der Aktinien und ihre differentialdiagnostische Bedeutung. Helgol Wiss Meeresunters 9:284–317

    Article  Google Scholar 

  • Schmidt H (1972) Die Nesselkapseln der Anthozoen und ihre Bedeutung fur die phylogenetische Systematik. Helgol Wiss Meeresunters 23:422–458

    Article  Google Scholar 

  • Schmidt H (1974) On evolution in the Anthozoa. In: Proceedings of the second international coral reef symposium 1 (October, 1974). Great Barrier Reef Committee, Brisbane, pp 533–560

  • Sebens KP, Laakso G (1977) The genus Tealia (Anthozoa: Actiniaria) in the waters of the San Juan Archipelago and the Olympic Peninsula. Wasmann J Biol 35:152–168

    Google Scholar 

  • Stephenson TA (1935) The British sea anemones, vol 2. Ray Society, London

    Google Scholar 

  • Stimpson W (1853) Synopsis of the marine invertebrata of Grand Manan: or the region about the mouth of the Bay of Fundy, New Brunswick. Smithsonian Institution, Washington

    Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Verrill AE (1922) The Actiniaria of the Canadian Arctic Expeditions, with notes on interesting species from Hudson Bay and other Canadian localities. Rep Can Arctic Exped 1913–1918(8):89–164

    Google Scholar 

  • Weill R (1934) Contribution à l'Étude des Cnidaires et de leurs Nématocystes. Les Presses Universitaires de France, Paris

    Google Scholar 

  • Widersten B (1976) Ceriantharia, Zoanthidea, Corallimorpharia, and Actiniaria from the continental shelf and slope off the eastern coast of the United States. Fish Bull 74:857–878

    Google Scholar 

  • Zhubicas II (1977) Some species of actinians of the western region of the Barents Sea and Franz Josef Land. Issledovania Fauni Morey 14:106–125

    Google Scholar 

Download references

Acknowledgements

The work was funded by the Russian Foundation for Basic Research (Grant № 16-04-01685 to E.S. Bocharova) and by the Natural Sciences and Engineering Research Council of Canada (NSERC; Grant No. 311406 to A. Mercier). The authors wish to thank E. Montgomery and the Ocean Sciences Field Services of Memorial University for their assistance with the animal collections, as well as the reviewers for their constructive comments.

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Authors and Affiliations

  1. Kamchatka Branch of Pacific Geographical Institute, Far-Eastern Branch of the Russian Academy of Sciences, 6 Partizanskaya Street, Petropavlovsk-Kamchatsky, Russia, 683000

    N. P. Sanamyan & K. E. Sanamyan

  2. Department of Ocean Sciences, Memorial University, St. John’s, NL, A1C 5S7, Canada

    A. Mercier

  3. Society for Exploration and Valuing of the Environment (SEVE), Portugal Cove-St. Phillips, NL, A1M 2B7, Canada

    J.-F. Hamel

  4. Koltzov Institute of Developmental Biology of Russian Academy of Sciences, 26 Vavilov Street, Moscow, Russia, 119334

    E. S. Bocharova

  5. Russian Federal Research Institute of Fisheries and Oceanography (VNIRO), 17 V. Krasnosel’skaya, Moscow, Russia, 107140

    E. S. Bocharova

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  1. N. P. Sanamyan
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Correspondence to E. S. Bocharova.

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Sanamyan, N.P., Sanamyan, K.E., Mercier, A. et al. Morphological and molecular assessment of large sea anemones (Actiniaria: Actiniidae) in Newfoundland (eastern Canada). Polar Biol 43, 495–509 (2020). https://doi.org/10.1007/s00300-020-02652-z

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