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CO2 biofixation and fatty acid composition of two indigenous Dunaliella sp. isolates (ABRIINW-CH2 and ABRIINW-SH33) in response to extremely high CO2 levels

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Abstract

Global warming, as a result of atmospheric CO2 increase, is regarded as an important universal concern. Microalgae are considered as appropriate microorganisms for CO2 assimilation. Here we aimed to investigate carbon biofixation ability of two indigenous isolates of Dunaliella spp. (ABRIINW-CH2 and ABRIINW-SH33) under elevated CO2 concentrations of 10, 20, and 30% (v/v) as well as their lipid content, productivity, and fatty acid profile under adjusted pH conditions. The maximum biomass production and CO2 biofixation rates were obtained under 10% CO2. High CO2 concentrations were favorable for the accumulation of lipids, lipid productivity, and polyunsaturated fatty acids formation. The highest lipid content and lipid productivity was obtained at 10% CO2. The highest fraction of the fatty acids (FA) profile was allocated to omega-3 FAs at 20% CO2. Accordingly, these isolates were able to tolerate extremely high CO2 concentrations and present even enhanced growth as well as formation of valuable products.

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Abbreviations

SFAs :

Saturated fatty acids

MUFAs :

Monounsaturated fatty acids

UFAs :

Unsaturated fatty acids

LC PUFAs :

Long-chain polyunsaturated fatty acids

DW :

Dry weight

DU :

Degree of unsaturation

n-3 :

Omega-3

n-6 :

Omega-6

ABRIINW :

Agricultural Biotechnology Research Institute of Iran West and Northwest Region

ROS :

Reactive oxygen species

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Acknowledgements

The financial support of this research was provided equally by Agricultural Biotechnology Institutes, Biotechnology Development Council of Iran and University of Tehran. Further, we would like to thank Nahid Hoseinzadeh for her helpful suggestions in preparing this paper.

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Authors and Affiliations

  1. Department of Plant Biology, and Center of Excellence in Phylogeny of Living Organisms in Iran, School of Biology, College of Science, University of Tehran, 14155, Tehran, Iran

    Roya Moghimifam, Vahid Niknam & Hassan Ebrahimzadeh

  2. Forests and Rangelands Research Department, East Azarbaijan Agricultural and Natural Resources Research and Education Center (AREEO), Tabriz, Iran

    Roya Moghimifam

  3. Department of Food Biotechnology, Branch for Northwest and West Region, Agricultural Education and Extension Organization (AREEO), Agricultural Biotechnology Research Institute of Iran, Tabriz, Iran

    Mohammad Amin Hejazi

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  1. Roya Moghimifam
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RM: performed experiments, analyzed data, and wrote the manuscript. VN: supervised the project. HE: helped supervise the project. MAH: supervised the project and co-wrote the paper. All authors provided critical feedback and helped shape the research, analysis, and manuscript.

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Correspondence to Vahid Niknam or Mohammad Amin Hejazi.

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Moghimifam, R., Niknam, V., Ebrahimzadeh, H. et al. CO2 biofixation and fatty acid composition of two indigenous Dunaliella sp. isolates (ABRIINW-CH2 and ABRIINW-SH33) in response to extremely high CO2 levels. Bioprocess Biosyst Eng 43, 1587–1597 (2020). https://doi.org/10.1007/s00449-020-02350-4

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