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Ctenophthalmus baeticus boisseauorum (Beaucournu, 1968) and Ctenophthalmus apertus allani (Smit, 1955) (Siphonaptera: Ctenophthalmidae) as synonymous taxa: morphometric, phylogenetic, and molecular characterization

Published online by Cambridge University Press:  27 April 2020

Antonio Zurita ,
Ángela María García-Sánchez  and
Cristina Cutillas Open the ORCID record for Cristina Cutillas [Opens in a new window]
Antonio Zurita
Affiliation:
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012Seville, Spain
Ángela María García-Sánchez
Affiliation:
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012Seville, Spain
Cristina Cutillas*
Affiliation:
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012Seville, Spain
*
Author for correspondence: Cristina Cutillas, Email: cutillas@us.es
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Abstract

The family Ctenophthalmidae (Order Siphonaptera) has been considered as a ‘catchall’ for a wide range of divergent taxa showing a paraphyletic origin. In turn, Ctenophthalmus sp. (Ctenophthalmidae) includes 300 valid described taxa. Within this genus, males are easily distinguishable basing on the size, shape, and chaetotaxy of their genitalia; however, females show slight morphological differences with each other. The main objective of this work was to carry out a comparative morphometric, phylogenetic, and molecular study of two different subspecies: Ctenophthalmus baeticus boisseauorum and Ctenophthalmus apertus allani in order to clarify and discuss its taxonomic status. From a morphological and biometrical point of view, we found clear differences between modified abdominal segments of males of both subspecies and slight differences in the margin of sternum VII of all female specimens which did not correspond with molecular and phylogenetic results based on four different molecular markers (Internal Transcribed Spacer 1 and 2 of ribosomal DNA, and the partial cytochrome c oxidase subunit 1 and cytochrome b of mitochondrial DNA). Thus, we observed a phenotypic plasticity between both subspecies, which did not correspond with a real genotypic variability nor different environmental or ecological conditions. Basing on these results, we could consider that there are no solid arguments to consider these two ‘morphosubspecies’ as two different taxa. We propose that C. b. boisseauorum should be considered as a junior synonym of C. a. allani.

Keywords

Ctenophthalmusmolecular biologyribosomal and mitochondrial DNASiphonapterataxonomy
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 110 , Issue 6 , December 2020 , pp. 663 - 676
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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