We studied the role of opioid receptor subtypes in improvement of the functional state of the heart during reperfusion after adaptation to continuous normobaric hypoxia. To this end, male Wistar rats were subjected to continuous normobaric hypoxia (12% O2). Then, the hearts were isolated and exposed to total 45-min ischemia followed by 30-min reperfusion. Opioid receptor antagonists were added to the perfusion solution prior to ischemia. It was found that continuous normobaric hypoxia reduced the release of creatine phosphokinase into the effluent, increased myocardial contractile force, and decreased the end-diastolic pressure during reperfusion; these positive effects were related to activation of cardiac δ2- and μ-opioid receptors.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alánová P, Chytilová A, Neckář J, Hrdlička J, Míčová P, Holzerová K, Hlaváčková M, Macháčková K, Papoušek F, Vašinová J, Benák D, Nováková O, Kolář F. Myocardial ischemic tolerance in rats subjected to endurance exercise training during adaptation to chronic hypoxia. J. Appl. Physiol. (1985). 2017;122(6):1452-1461.
Braunwald E. The war against heart failure: the Lancet lecture. Lancet. 2015;385:812-824.
Devidze N, Zhang Q, Zhou J, Lee AW, Pataky S, Kow LM, Pfaff DW. Presynaptic actions of opioid receptor agonists in ventromedial hypothalamic neurons in estrogen- and oil-treated female mice. Neuroscience. 2008;152(4):942-949.
Martin NA, Terruso MT, Prather PL. Agonist Activity of the delta-antagonists TIPP and TIPP-psi in cellular models expressing endogenous or transfected delta-opioid receptors. J. Pharmacol. Exp. Ther. 2001;298(1):240-248.
Maslov LN, Naryzhnaya NV, Prokudina ES, Kolar F, Gorbunov AS, Zhang Y, Wang H, Tsibulnikov SY, Portnichenko AG, Lasukova TV, Lishmanov YB. Preserved cardiac mitochondrial function and reduced ischaemia/reperfusion injury afforded by chronic continuous hypoxia: role of opioid receptors. Clin. Exp. Pharmacol. Physiol. 2015;42(5):496-501.
Maslov LN, Naryzhnaia NV, Tsibulnikov SY, Kolar F, Zhang Y, Wang H, Gusakova AM, Lishmanov YB. Role of endogenous opioid peptides in the infarct size-limiting effect of adaptation to chronic continuous hypoxia. Life Sci. 2013;93(9- 11):373-379.
Sánchez-Blázquez P, Rodríguez-Díaz M, Frejo MT, Garzón J. Stimulation of mu- and delta-opioid receptors enhances phosphoinositide metabolism in mouse spinal cord: evidence for subtypes of delta-receptors. Eur. J. Neurosci. 1999;11(6):2059- 2064.
Stevens WC Jr, Jones RM, Subramanian G, Metzger TG, Ferguson DM, Portoghese PS. Potent and selective indolomorphinan antagonists of the kappa-opioid receptor. J. Med. Chem. 2000;43(14):2759-2769.
Tsibulnikov SY, Maslov LN, Naryzhnaya NV, Ma H, Lishmanov YB, Oeltgen PR, Garlid K. Role of protein kinase C, PI3 kinase, tyrosine kinases, NO-synthase, KATP channels and MPT pore in the signaling pathway of the cardioprotective effect of chronic continuous hypoxia. Gen. Physiol. Biophys. 2018;37(5):537-547.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 12, pp. 683-686, December, 2019
Rights and permissions
About this article
Cite this article
Naryzhnaya, N.V., Prokudina, E.S., Nesterov, E.A. et al. The Role of Cardiac Opioid Receptors in the Cardioprotective Effect of Continuous Normobaric Hypoxia. Bull Exp Biol Med 168, 727–729 (2020). https://doi.org/10.1007/s10517-020-04789-7
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-020-04789-7