NoteA new recombinant F1 antigen as a cost and time-effective tool for plague diagnosis
Section snippets
Authors contributions
Diego H.C. Tavares and Matheus F. Bezerra produced and purified the recombinant F1 antigen and performed hemagglutination tests. Thaíse Y.V.L. Cavalcanti, Camila C. Xavier and Diego H.C. Tavares cloned the caf1 gene and performed western blotting. Matheus F. Bezerra, Alzira M.P. Almeida and Christian R.S. Reis wrote the manuscript and made the figures. Nilma C. Leal, Franklin B. Magalhães, Alzira M.P. Almeida and Christian R.S. Reis conceived and designed the study. Christian R.S. Reis and
Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgments
The authors would like to thank the Program for Technical Development of Health Inputs-PDTIS-Fiocruz from the Institute Aggeu Magalhaes – Fiocruz PE for the use of its facilities, the automatic sequencing RPT01C and the BSL-3 laboratory. We acknowledge Cláudio Cavalcanti, Silvana Vasconcelos and Fabiana Laura from the Department of Microbiology for technical assistance. We also would like to thank Danilo Xavier for information about peptide signal of bacterial proteins, Gustavo Lima for support
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Performance assessment of a new indirect rapid diagnostic test for plague detection in humans and other mammalian hosts
2022, Acta TropicaCitation Excerpt :To evaluate cross-reactions with other pathogens, we included canine sera positive for Ehrlichia canis and Anaplasma platys (both diagnosed with the SNAP 4Dx Idexx test); Leishmania infantum (diagnosed with the DPP® - Dual Path Platform Leishmaniose Canina and EIE - Leishmaniose Visceral Canina Bio-Manguinhos test) as well as rabbits immunized with formol-killed Yersinia pseudotuberculosis and Yersinia enterocolitica. The 25 positive control sera were derived from rabbits immunized with formol-killed Y. pestis cultures or with the purified F1 antigen, as previously described (Tavares et al., 2020). Three of those were from rabbits immunized with the reference EV76 or A1122 Y. pestis strains in independent experiments, 18 were immunized with diverse Brazilian Y. pestis strains from the Fiocruz/CYP bacterial culture collection maintained at the IAM (http://cyp.fiocruz.br), and four were immunized with the F1 antigen.
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2023, Zoonoses (Ireland)Development of a dual antigen lateral flow immunoassay for detecting Yersinia pestis
2022, PLoS Neglected Tropical DiseasesEvaluation of a multi-species Protein A-ELISA assay for plague serologic diagnosis in humans and other mammal hosts
2022, PLoS Neglected Tropical Diseases
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These authors contributed equally to this work