Abstract
A novel bacterial strain, designated YIM 132548 T, was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China. The organism was Gram-stain negative, aerobic and methylotrophic. The cell was catalase positive and oxidase negative, asporogenous, rod-shaped and motile with three polar flagella. The strain could grow at 15–30 °C (optimum, 20 °C), at pH 6.0–9.0 (optimum, pH 7.0) and does not grow in the presence of NaCl. According to the 16S rRNA gene sequence analysis, strain YIM 132548 T showed high levels of 16S rRNA gene sequence similarity with Methylobacterium soli YIM 48816 T (97.6%) and Methylobacterium durans NBRC 112876 T (97.3%), less than 97.0% with other validly named type strains of the genus Methylobacterium. Ubiquinone Q-10 was the predominant respiratory ubiquinone. The predominant cellular fatty acid was identified as summed feature 8 (C18:1ω7c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G + C content of the draft genome sequence is 70.2 mol%. The average nucleotide identity and digital DNA–DNA hybridizations values of strain YIM 132548 T with M. soli YIM 48816 T and M. durans NBRC 112876 T were 87.0% and 82.0%, 40.6% and 27.2% based on draft genome sequences, respectively. On the basis of phylogenetic, chemotaxonomic, phenotypic and genomic data, strain YIM 132548 T is concluded to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium planium sp. nov. is proposed. The type strain is YIM 132548 T (= CGMCC 1.17323 T = NBRC 114056 T).
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Abbreviations
- DPG:
-
Diphosphatidylglycerol
- PG:
-
Phosphatidylglycerol
- PC:
-
Phosphatidylcholine
- PME:
-
Phosphatidylmethylethanolamine
- PE:
-
Phosphatidylethanolamine
- AL:
-
Unidentified aminolipid
- L:
-
Lipid
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization
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Acknowledgements
This research was funded by National Natural Science Foundation of China (31460005) and Major research project of Guangxi for science and technology (AA18242026).
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LJ performed the experiments and wrote the manuscript; XW collected the lichen samples; KZ and DFA analysed the data; GL and LL analysed the data; LW identified the lichen samples; YJ guided the experiments and revised the manuscript; CJ designed the study.
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Communicated by Erko Stackebrandt.
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The GenBank/EMBL/DDBJ Accession Number for the 16S rRNA gene sequence of strain YIM 132548T is MN317338 and the genome sequence is VZZJ00000000.
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Jiang, L., An, D., Wang, X. et al. Methylobacterium planium sp. nov., isolated from a lichen sample. Arch Microbiol 202, 1709–1715 (2020). https://doi.org/10.1007/s00203-020-01881-4
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DOI: https://doi.org/10.1007/s00203-020-01881-4