We studied the correlations of the expression of Ki-67 proliferation marker and localization of heat shock protein Hsp70 in tumor cells of papillary thyroid cancer with some clinical and morphological parameters and a 10-year survival prognosis. High variability of the proliferative activity (by Ki-67 index) of tumor cells was revealed. It was found that Hsp70 protein has different localization in tumor cells: cytoplasmic, nuclear, nucleolar, and mixed. Nuclear translocation of the Hsp70 protein correlated with the stage of the process and the prognosis of the disease, but did not correlate with sex, tumor size, capsule invasion, and lymph node metastasis. Ki-67 index of tumor cells correlated with sex, stage of the process, size of tumor formation and prognosis, but did not correlated with age, invasion into the capsule and metastasis to the lymph nodes. Depending on the variants of Hsp70 and Ki-67 co-expression, three molecular types of papillary thyroid cancer can be distinguished: favorable, intermediate, and poor prognosis.
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References
TNM. Classification of Malignant Tumors. Sobin LH, Gospodarowicz MK, Wittekind Ch, eds. Moscow, 2011. Russian.
Chen X, Ehrhardt WM, Halberg RB, Aronow BJ, Dove WF. Cellular expression patterns of genes upregulated in murine and human colonic neoplasms. J. Histochem. Cytochem. 2008;56(5):433-441.
Garrido C, Brunet M, Didelot C, Zermati Y, Schmitt E, Kroemer G. Heat shock proteins 27 and 70: Anti-apoptotic proteins with tumorigenic properties. Cell Cycle. 2006;5(22):2592-2601.
Ito Y, Miyauchi A, Kakudo K, Hirokawa M, Kobayashi K, Miya A. Prognostic significance of Ki-67 labeling index in papillary thyroid carcinoma. World J. Surg. 2010;34(12):3015-3021.
Ito Y, Uruno T, Takamura Y, Miya A, Kobayashi K, Matsuzuka F, Kuma K, Miyauchi A. Papillary microcarcinomas of the thyroid with preoperatively detectable lymph node metastasis show significantly higher aggressive characteristics on immunohistochemical examination. Oncology. 2005;68(2-3):87-96.
Jolly C, Morimoto RI. Role of the heat shock response and molecular chaperones in oncogenesis and cell death. J. Natl Cancer Res. 2000;92(19):1564-1572.
Juhasz K, Lipp AM, Nimmervoll B, Sonnleitner A, Hesse J, Haselgruebler T, Balogi Z. The Complex function of Hsp70 in metastatic cancer. Cancers (Basel). 2013;6(1):42-66.
Longshaw VM, Chapple JP, Balda MS, Cheetham ME, Blatch GL. Nuclear translocation of the Hsp70/Hsp90 organizing protein mSTI1 is regulated by cell cycle kinases. J. Cell Sci. 2004;117(Pt 5):701-710.
Lorenzato M, Abboud P, Lechki C, Browarnyj F, O’Donohue MF, Ploton D, Adnet JJ. Proliferation assessment in breast cancer: a double-staining technique for AgNOR quantification in MIB-1 positive cells especially adapted for image cytometry. Micron. 2000;31(2):151-159.
Malusecka E, Zborek A, Krzyzowska-Gruca S, Krawczyk Z. Immunohistochemical detection of the inducible heat shock protein Hsp70: A methodological study. J. Histochem. Cytochem. 2006;54(2):183-190.
Mosser DD, Morimoto RI. Molecular chaperones and the stress of oncogenesis. Oncogene. 2004;23(16):2907-2918.
Nakayama H, Yoshida A, Nakamura Y, Hayashi H, Miyagi Y, Wada N, Rino Y, Masuda M, Imada T. Clinical significance of BRAF (V600E) mutation and Ki-67 labeling index in papillary thyroid carcinomas. Anticancer Res. 2007;27(5B):3645-3649.
Papotti M, Torchio B, Grassi L, Favero A, Bussolati G. Poorly differentiated oxyphilic (Hurtle cell) carcinomas of the thyroid. Am. J. Surg. Pathol. 1996;20(6):686-694.
Sobrinho-Simões M. Hail to the histologic grading of papillary thyroid carcinoma? Cancer. 2000;88(8):1766-1768.
van Loo G, Saelens X, van Gurp M, MacFarlane M, Martin SJ, Vandenabeele P. The role of mitochondrial factors in apoptosis: a Russian roulette with more than one bullet. Cell Death Differ. 2002;9(10):1031-1042.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 11, pp. 638-644, November, 2019
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Avdalyan, A.M., Ivanov, A.A., Lushnikova, E.L. et al. The Relationship of Immunoexpression of Ki-67 and Hsp70 with Clinical and Morphological Parameters and Prognosis of Papillary Thyroid Cancer. Bull Exp Biol Med 168, 688–693 (2020). https://doi.org/10.1007/s10517-020-04781-1
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DOI: https://doi.org/10.1007/s10517-020-04781-1