In experimental rabbits, cysteine injected intravenously in a dose of 1000 mg/kg temporarily bound zinc in β cells and prevented the formation of chelate zinc complexes in response to subsequent injection of diabetogenic zinc-binding substances that induce cell destruction. Injection of cysteine to animals was associated with a sharply negative reaction to zinc in β cells, which attests to blockade of zinc ions. Injection of cysteine few minutes after dithizone and formation of zinc—dithizone complex was followed by displacement of dithizone from the complex and prevented the development of diabetes in most animals. The most plausible mechanism of preventive effect of cysteine is the formation of 2:1 zinc—cysteine complex in β cells with possible fixation of Zn atom between sulfur atoms from SH groups of two cysteine molecules.
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References
Meyramova AG. Diabetogenic zinc-binding β-cytotoxic compounds. Probl. Endokrinol. 2003;49(2):8-16. Russian.
Meyramov GG, Konert KD, Meyramova AG. Diabetogenic effect of xanthurenic acid. Probl. Endokrinol. 2001;47(1):39-44. Russian.
Dunn MF. Zinc-ligand interactions modulate assembly and stability of the insulin hexamer — a review. Biometals. 2005;18(4):295-303.
Li YV. Zinc and insulin in pancreatic beta-cells. Endocrine. 2014;45(2):178-189.
Maret W. Zinc in pancreatic islet biology insulin sensitivity, and diabetes. Prev. Nutr. Food. 2017;22(1):1-8.
Meyramov GG, Kikimbaeva AA, Meyramova AG. Fluorescent histochemical method for staining of insulin in B-cells of isolated pancreatic islets by diethylpseudoisocyanine chloride. Acta Diabetol. 2005;42(1):66.
Meyramov GG, Kikimbaeva AA, Meyramova AG. Victoria 4R Method Staining of Insulin in B-cells of Isolated Pancreatic Islets. Acta Diabetol. 2003;40(4):208.
Meyramova AG, Kohnert KD, Aitkulov AM, Alina AR, Zhuzbaeva GO, Kystaubaeva ZT, Andreewa AP, Kovalenko OL, Zhuzshasarova AA, Tyrzhanova SS. High specific fluorescent method for staining of zinc-insulin complex in pancreatic B-cells. Bull. Karaganda State University. 2013;(4):78-84.
Meyramov GG, Kohnert KD, Kikimbaeva AA, Dupont ON, Aitkulov AM, Kystaubaeva ZT, Tykezhanova GM, Turgunva LG, Laryushina EM, Abdraimova-Meyramova AG, Shaybek AS, Zhumasheva KA, Tyrshanva SS. Gluthation’ reduced form protect B-cells from destruction caused diabetogenic ligands. Diabetes. 2015;64(7, Suppl. 1):A735.
Meyramov GG, Meyramova AG. 8PTSQ as fluorescent reagent for revealing of Zn-ions in B-cells and as diabetogenic chelator. Acta Diabetol. 2003;40(1):57.
Meyramov GG, Meyramova AG. Prevention of diabetes induced by chelators by L-hystidine. Diabetes. 2004;52(6, Suppl. 1):583.
Rubino FM. Toxicity of glutathione-binding metals: a review of targets and mechanisms. Toxics. 2015;3(1):20-62.
Wohlrab F, von Dorsche HH, Krautschick I, Schmidt S. On the specificity of insulin staining by Victoria blue 4R. Histochem. J. 1985;17(5):515-518.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 11, pp. 559-564, November, 2019
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Meyramov, G.G., Kohnert, KD., Shaybek, A.Z. et al. Cysteine Prevents the Development of Experimental Diabetes Induced by Zinc-Binding Substances. Bull Exp Biol Med 168, 621–626 (2020). https://doi.org/10.1007/s10517-020-04765-1
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DOI: https://doi.org/10.1007/s10517-020-04765-1