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Antimicrobial and Antioxidant Effects of a Forest Actinobacterium V002 as New Producer of Spectinabilin, Undecylprodigiosin and Metacycloprodigiosin

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Abstract

The aim of the study is the research and identification of a Streptomyces strain as a new producer of spectinabilin, undecylprodigiosin and metacycloprodigiosin. Among 54 actinomycete isolates isolated from El-Ogbane forest soils in Algeria, only one isolate, designated V002, was selected for its ability to produce prodigiosins. The selected strain was analysed for its ability to produce three different secondary metabolites as well as their biological activities. V002 belongs to the Streptomyces genus and has significant antimicrobial and antioxidant activities. The taxonomic position of V002 by 16S rRNA sequence analysis showed a similarity of 99.93% with Streptomyces lasiicapitis DSM 103124T and 98.96% with Streptomyces spectabilis DSM 40512T. Fractionation of crude secondary metabolites produced by the strain using HPLC–MS revealed the presence of spectinabilin, undecylprodigiosin and metacycloprodigiosin, which demonstrated significant activity. Strain V002 is considered a new producer of spectinabilin, undecylprodigiosin and metacycloprodigiosin with significant antimicrobial and antioxidant activity.

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Acknowledgements

We are thankful to the researchers and technician from microbial strain collection department, Helmholtz Centre for Infection Research for their support during this work.

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Authors and Affiliations

  1. Department of Microbial Strain Collection, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany

    Mohamed Amine Gacem & Joachim Wink

  2. Laboratory of Ecosystems Protection in Arid and Semi-Arid Area, University of Kasdi Merbah, 30000, Ouargla, Algeria

    Mohamed Amine Gacem & Aminata Ould-El-Hadj-Khelil

  3. Department of Biology, Faculty of Science, University of Amar Tlidji, 03000, Laghouat, Algeria

    Mohamed Amine Gacem & Badreddine Boudjemaa

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  1. Mohamed Amine Gacem
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  2. Aminata Ould-El-Hadj-Khelil
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MAG carried out experimental work. MAG and BB co-wrote the manuscript. AOHK and JW conceived and designed the study. All authors contributed to interpretation of results, read and approved the final draft.

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Correspondence to Mohamed Amine Gacem.

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Gacem, M.A., Ould-El-Hadj-Khelil, A., Boudjemaa, B. et al. Antimicrobial and Antioxidant Effects of a Forest Actinobacterium V002 as New Producer of Spectinabilin, Undecylprodigiosin and Metacycloprodigiosin. Curr Microbiol 77, 2575–2583 (2020). https://doi.org/10.1007/s00284-020-02007-1

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