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Lipase-catalyzed synthesis of propyl-phenyl acetate: a kinetic and thermodynamic study

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Abstract

This study focuses on the synthesis of propyl-phenyl acetate via esterification reaction in the presence of immobilized Candida antartica lipase-B (CAL-B). In this work, the effect of relevant factors (kinetics and thermodynamic) on total percent conversion and process optimization was studied. The reaction was performed in heptane medium with 1:2 molar ratio of benzoic acid: n-propanol with 0.6% (w/v) biocatalyst loading at 40 °C to attain a maximum conversion of 96.1% within 40 min of reaction time. Effect of increase in temperature on ∆G values indicates that lipase is more promising at moderate temperature (40 °C). A second-order kinetic model was proposed to evaluate apparent kinetic constants that indicate a good agreement between the experimental and theoretical data (0.94 ≤ R2 ≤ 0.99) with high initial reaction rate (113.5 mM/min). Finally, the catalyst CAL-B was successfully reused eight times without any significant decrease in relative activity.

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Acknowledgements

The authors would like to acknowledge the University Grants Commission (UGC) of India for financial support in the research work.

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  1. Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai, 400019, India

    Prerana D. Tomke & Virendra K. Rathod

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  1. Prerana D. Tomke
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  2. Virendra K. Rathod
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Correspondence to Virendra K. Rathod.

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Tomke, P.D., Rathod, V.K. Lipase-catalyzed synthesis of propyl-phenyl acetate: a kinetic and thermodynamic study. Bioprocess Biosyst Eng 43, 1659–1670 (2020). https://doi.org/10.1007/s00449-020-02358-w

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  • DOI: https://doi.org/10.1007/s00449-020-02358-w

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